Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept

Inês Leal Reis; Bruna Lopes; Patrícia Sousa; Ana Catarina Sousa; Mariana Branquinho; Ana Rita Caseiro; Alexandra Rêma; Inês Briote; Carla Mendonça; Jorge Miguel Santos; Luís Atayde; Rui Alvites; Ana Colette Maurício

doi:10.20944/preprints202311.1453.v1

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preprints.org > medicine and pharmacology > veterinary medicine > doi: 10.20944/preprints202311.1453.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept

Version 1 : Received: 21 November 2023 / Approved: 23 November 2023 / Online: 23 November 2023 (04:58:38 CET)

A peer-reviewed article of this Preprint also exists.

Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.V.; Caseiro, A.R.; Rêma, A.; Briote, I.; Mendonça, C.M.; Santos, J.M.; Atayde, L.M.; Alvites, R.D.; Maurício, A.C. Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept. Animals 2024, 14, 370. Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.V.; Caseiro, A.R.; Rêma, A.; Briote, I.; Mendonça, C.M.; Santos, J.M.; Atayde, L.M.; Alvites, R.D.; Maurício, A.C. Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept. Animals 2024, 14, 370.

Abstract

Horses are high performance athletes prone to sportive injuries such as tendonitis and desmitis. The formation of fibrous tissue in tendon repair becomes a therapeutic challenge to overcome. This impels regenerative medicine to develop innovative therapies that enhance tissue regenera-tion retrieving original tissue properties. Multipotent Mesenchymal Stem/ Stromal Cells (MSCs) have been successfully used to develop therapeutic products, as they secrete a variety of bioac-tive molecules that play a pivotal role in tissue regeneration. These factors are released in cul-ture media producing conditioned media (CM). The aforementioned assumptions led to the formulation of equine synovial membrane MSCs (eSM-MSCs) – the cellular pool that naturally regenerates joint tissue, combined with medium enriched in immunomodulatory factors (among other bioactive factors) produced by umbilical cord stroma-derived MSCs (eUC-MSCs), that nat-urally contribute to suppress the immune rejection in the maternal-fetal barrier. A clinical case of an equine acute desmitis, treated with this formulation is presented. Ultrasonographic ligament regeneration occurred in a reduced time frame, reducing stoppage time and allowing return to unrestricted competition after completion of a physical rehabilitation program. This study fo-cused on the determination of the formulation therapeutic potential and the evaluation of its synergistic effect in an equine desmitis treatment, utilizing the cells themselves and its secretome.

Keywords

Allogenic; Case study; Ligament; Mesenchymal stem cells; MSC-based therapies; Secretome; Sport horses; Synovial membrane mesenchymal stem cell; Umbilical cord conditioned medium

Subject

Medicine and Pharmacology, Veterinary Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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