Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Sensitive and Specific Monoclonal Antibody based Enzyme‐Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment

Linwei Zhang
,
Xiaoyang Yin
,
Hongfei Yang
,
Hao Wen
,
Shiyun Han
,
Xiaoming Pan
,
Huaming Li
,
Liangni Qin
,
Jiaxu Xiao
ORCID logo ,
Long Li
,
Yu Si
,
Jixiang Liang
,
Dapeng Peng
*
Version 1 : Received: 22 November 2023 / Approved: 22 November 2023 / Online: 22 November 2023 (16:49:06 CET)

A peer-reviewed article of this Preprint also exists.

Zhang, L.; Yin, X.; Yang, H.; Wen, H.; Han, S.; Pan, X.; Li, H.; Peng, D. A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment. Foods 2024, 13, 12. Zhang, L.; Yin, X.; Yang, H.; Wen, H.; Han, S.; Pan, X.; Li, H.; Peng, D. A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment. Foods 2024, 13, 12.

Abstract

Pretilachlor is a chloroacetamide herbicide, mainly used for the control of weeds and broadleaf weeds in rice, and widely used in China. For the detection of residues of pretilachlor in the en-vironment and food, a highly sensitive and specific monoclonal antibody against pretilachlor was prepared, and the half maximum inhibitory concentration (IC50) of the monoclonal antibody was validated to be 31.47±2.35 μg/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25~100 μg/L was developed for the detection of pretilachlor residues in the environment and food crops. The specificity of the antibody was explained by computer simula-tions and experimental validation. No cross-reactivity of this monoclonal antibody to alachlor, acetochlor and metalaxyl, and it cross-reacted less than 3.0% to both butachlor and propisochlor. The limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.83~5.23 μg/L. The recoveries of all samples were 78.3%~91.3%. The ic-ELISA method was validated by high-performance liquid chromatography, thus, it can be used for residue detection of pretilachlor in the environment and grains.

Keywords

residue detection; pretilachlor; monoclonal antibody; ic-ELISA

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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