Version 1
: Received: 22 November 2023 / Approved: 22 November 2023 / Online: 22 November 2023 (05:51:03 CET)
How to cite:
Firoozbahr, M.; Palombo, E.A.; Kingshott, P.; Zaferanloo, B. Evaluation of Antibacterial and Antibiofilm Properties in Australian Native Plant Endophyte Metabolites for Wound care Applications. Preprints2023, 2023111391. https://doi.org/10.20944/preprints202311.1391.v1
Firoozbahr, M.; Palombo, E.A.; Kingshott, P.; Zaferanloo, B. Evaluation of Antibacterial and Antibiofilm Properties in Australian Native Plant Endophyte Metabolites for Wound care Applications. Preprints 2023, 2023111391. https://doi.org/10.20944/preprints202311.1391.v1
Firoozbahr, M.; Palombo, E.A.; Kingshott, P.; Zaferanloo, B. Evaluation of Antibacterial and Antibiofilm Properties in Australian Native Plant Endophyte Metabolites for Wound care Applications. Preprints2023, 2023111391. https://doi.org/10.20944/preprints202311.1391.v1
APA Style
Firoozbahr, M., Palombo, E.A., Kingshott, P., & Zaferanloo, B. (2023). Evaluation of Antibacterial and Antibiofilm Properties in Australian Native Plant Endophyte Metabolites for Wound care Applications. Preprints. https://doi.org/10.20944/preprints202311.1391.v1
Chicago/Turabian Style
Firoozbahr, M., Peter Kingshott and Bita Zaferanloo. 2023 "Evaluation of Antibacterial and Antibiofilm Properties in Australian Native Plant Endophyte Metabolites for Wound care Applications" Preprints. https://doi.org/10.20944/preprints202311.1391.v1
Abstract
The wound management sector has attracted considerable attention due to the increasing challenge posed by antimicrobial resistance (AMR) and the specialized care required for chronic wounds. Effective wound care involves selecting appropriate dressings tailored with antimicrobial agents to prevent bacterial infection. Among various types of wound dressings, fibers and electrospun fibers, with their unique characteristics, have emerged as innovative new materials. However, the rise of AMR necessitates the exploration of new antimicrobial agents for wound dressings, particularly for addressing bacterial pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Endophytic fungi, known for producing diverse bioactive compounds, including novel antibacterial agents, represent a promising source of such new agents. This study tested thirty-two endophytic fungi from thirteen distinct Australian native plants for their antibacterial activity against S. aureus, the most common wound-associated bacteria. Ethyl acetate (EtOAc) extracts from fungal culture filtrates exhibited inhibitory effects against both methicillin-sensitive S. aureus ATCC 25923 and MRSA strains M173525 and M180920. DNA sequence analysis was employed for fungal identification, with EtOAc extracts from the most active sample, EL 19 (Chaetomium globosum), selected for further bactericidal and antibiofilm testing against the chosen bacteria. Biofilm of S. aureus ATCC 25923 was reduced by 55% by EtOAc extracts of EL 19 at 1/2 × MIC. To demonstrate the potential application in wound dressing materials, three different concentrations of the extract were incorporated into Polycaprolactone fiber mats through electrospinning, with resultant inhibition of S. aureus ATCC 25923 being observed. This research underscores the potential of endophytic fungi from Australian plants as sources of substances effective against common wound pathogens. Further exploration of the responsible compounds and their mechanisms could facilitate the development of wound dressings effective against MRSA and innovative biofilm-resistant electrospun fibers, contributing to the global efforts to combat AMR.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
