Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

OsMYB58 Negatively Regulates Phosphate Acquisition via OsmiR399-Dependent Phosphate Starvation Signaling in Rice

Dongwon Baek
ORCID logo ,
Won Tae Yang
ORCID logo ,
Soyeon Hong
,
Hye Jeong Kim
,
Sunok Moon
,
Ki Hong Jung
ORCID logo ,
Doh Hoon Kim
* ORCID logo
Version 1 : Received: 22 November 2023 / Approved: 22 November 2023 / Online: 22 November 2023 (06:41:48 CET)

A peer-reviewed article of this Preprint also exists.

Baek, D.; Hong, S.; Kim, H.J.; Moon, S.; Jung, K.H.; Yang, W.T.; Kim, D.H. OsMYB58 Negatively Regulates Plant Growth and Development by Regulating Phosphate Homeostasis. Int. J. Mol. Sci. 2024, 25, 2209. Baek, D.; Hong, S.; Kim, H.J.; Moon, S.; Jung, K.H.; Yang, W.T.; Kim, D.H. OsMYB58 Negatively Regulates Plant Growth and Development by Regulating Phosphate Homeostasis. Int. J. Mol. Sci. 2024, 25, 2209.

Abstract

Phosphate (Pi) starvation is a critical factor limiting crop growth, development, and productivity. Rice (Oryza sativa) R2R3-MYB transcription factors function in the transcriptional regulation of plant responses to various abiotic stresses and micronutrient deprivation, but little is known about their roles in Pi starvation signaling and Pi homeostasis. Here we identified the R2R3-MYB transcription factor gene OsMYB58, which shares high sequence similarity with AtMYB58. OsMYB58 expression was induced more strongly by Pi starvation than by other micronutrient deficiencies. Overexpressing OsMYB58 in Arabidopsis thaliana and rice inhibited plant growth and development under Pi-deficiency conditions. In addition, overexpression of OsMYB58 in plants exposed to Pi deficiency strongly affected root development, including seminal root, lateral root, and root hair formation. Overexpressing OsMYB58 strongly decreased the expression of the rice microRNAs, OsmiR399a and OsmiR399j. By contrast, overexpressing OsMYB58 strongly increased the expression of rice PHOSPHATE 2 (OsPHO2), whose expression is repressed by miR399 during Pi starvation signaling. OsMYB58 functions as a transcriptional repressor of the expression of its target genes, as determined by a transcriptional activity assay. These results demonstrate that OsMYB58 negatively regulates OsmiR399-dependent Pi starvation signaling by enhancing OsmiR399s expression.

Keywords

MYB transcription factor; phosphate starvation signaling; OsMYB58; OsmiR399; PHR-miR399-PHO2

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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