PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota
Version 1
: Received: 21 November 2023 / Approved: 22 November 2023 / Online: 22 November 2023 (05:58:27 CET)
How to cite:
Mujahidy, S.M.J.; Ikeo, K.; Saito, K.; Ibn Sina, D.A.A.; Uddin, M.E. A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota. Preprints2023, 2023111374. https://doi.org/10.20944/preprints202311.1374.v1
Mujahidy, S.M.J.; Ikeo, K.; Saito, K.; Ibn Sina, D.A.A.; Uddin, M.E. A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota. Preprints 2023, 2023111374. https://doi.org/10.20944/preprints202311.1374.v1
Mujahidy, S.M.J.; Ikeo, K.; Saito, K.; Ibn Sina, D.A.A.; Uddin, M.E. A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota. Preprints2023, 2023111374. https://doi.org/10.20944/preprints202311.1374.v1
APA Style
Mujahidy, S.M.J., Ikeo, K., Saito, K., Ibn Sina, D.A.A., & Uddin, M.E. (2023). A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota. Preprints. https://doi.org/10.20944/preprints202311.1374.v1
Chicago/Turabian Style
Mujahidy, S.M.J., Dr. Abu Ali Ibn Sina and Md. Ekhlas Uddin. 2023 "A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota" Preprints. https://doi.org/10.20944/preprints202311.1374.v1
Abstract
The goal of the current study was to discover a novel potential probiotic strain of Lactobacillus spp. with anti-Escherichia coli activity from locally produced yogurt in Tongi, Gazipur, Bangladesh, compare its antagonistic activity with a commercial probiotic mixture of several strains, and approve a novel method for confirming the viability and relative abundance of the microbial community in a probiotic mixer. We carried out 16S sequencing, 16S metagenomics, Transmission Electron Microscopy (TEM) analysis, and other in vitro laboratory experiments to reach this objective. The strain TY-11 was identified as Lactobacillus delbrueckii subsp. indicus (16S sequence accession number OQ652026). It was gram-positive, anaerobic, lactose fermenting, and round-ended rod that typically measured 0.7 to 1.3 µm by 2.2 to 9 µm. In addition to having seven probiotic characteristics, it also showed an antagonistic impact on six different pathogens, but what's more noteworthy is that E. coli was the pathogen it inhibited most strongly (inhibition zone diameter was 18.88±0.18 mm). The most important and ground-breaking finding of this work was determining the probiotic features of a new probiotic strain, TY-11, whose antibacterial activity was virtually as effective as that of the probiotic combination with three different strains. Furthermore, the results of 16S high throughput sequencing and the conventional plate count method demonstrated a strong correlation (0.999) at the genus level, indicating that the use of both of these approaches in combination may be a practical way to assess the relative abundance of the microbial community and their viability in commercially available probiotic blends.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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