Wang, H.; Xiao, B.; Chen, S.; He, J.; Li, C. Identification of an Ortholog of MALT1 from Shrimp That Induces NF-κB-Mediated Antiviral Immunity. Viruses2023, 15, 2361.
Wang, H.; Xiao, B.; Chen, S.; He, J.; Li, C. Identification of an Ortholog of MALT1 from Shrimp That Induces NF-κB-Mediated Antiviral Immunity. Viruses 2023, 15, 2361.
Wang, H.; Xiao, B.; Chen, S.; He, J.; Li, C. Identification of an Ortholog of MALT1 from Shrimp That Induces NF-κB-Mediated Antiviral Immunity. Viruses2023, 15, 2361.
Wang, H.; Xiao, B.; Chen, S.; He, J.; Li, C. Identification of an Ortholog of MALT1 from Shrimp That Induces NF-κB-Mediated Antiviral Immunity. Viruses 2023, 15, 2361.
Abstract
MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1) serves as a pivotal mediator for NF-κB activation in response to a wide spectrum of transmembrane receptor stimuli. In the present study, a homolog of MALT1 is cloned from the Pacific white shrimp (Litopenaeus vannamei) named LvMALT1, and its potential function in shrimp innate immunity are explored. The open reading frame of LvMALT1 is 2364 bp that encodes 787 amino acids. The predicted LvMALT1 protein structure comprises a death domain, three immunoglobulin domains, and a caspase-like domain, exhibiting remarkable similarity to other homologs. LvMALT1 is a cytoplasmic-localized protein and could interact with LvTRAF6. Overexpression of LvMALT1 induces the activation of promoter elements governing the expression of several key antimicrobial peptides (AMPs), including Penaeidins (PENs) and Crustins (CRUs). Conversely, silencing of LvMALT1 leads to a reduction in the phosphorylation levels of Dorsal and Relish, along with a concomitant decline in the in vivo expression levels of multiple AMPs. Furthermore, LvMALT1 is prominently upregulated in response to a challenge by the white spot syndrome virus (WSSV), facilitating the NF-κB-mediated expression of AMPs as a defense against viral infection. Taken together, we identified a MALT1 homolog from shrimp L. vannamei, which plays a positive role in the TRAF6/NF-κB/AMPs axis mediated innate immunity.
Keywords
MALT1; Litopenaeus vannamei; WSSV; AMPs; NF-κB
Subject
Biology and Life Sciences, Aquatic Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
