Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

PCR Array Profiles the Expression of miRNAs Involved in Differentiation of Amniotic Fluid Stem Cells towards Endothelial and Smooth Muscle Progenitor Cells

Florin Iordache
* ,
Adriana Ionescu Petcu
ORCID logo ,
Aurelia Magdalena Pisoschi
ORCID logo ,
Loredana Stanca
,
Ovidiu Ionut Geicu
,
Liviu Bilteanu
,
Carmen Curutiu
ORCID logo ,
Bogdan Amuzescu
,
Andreea Iren Serban
ORCID logo
Version 1 : Received: 10 November 2023 / Approved: 20 November 2023 / Online: 22 November 2023 (15:08:37 CET)

A peer-reviewed article of this Preprint also exists.

Iordache, F.; Petcu, A.I.; Pisoschi, A.M.; Stanca, L.; Geicu, O.I.; Bilteanu, L.; Curuțiu, C.; Amuzescu, B.; Serban, A.I. PCR Array Profiling of miRNA Expression Involved in the Differentiation of Amniotic Fluid Stem Cells toward Endothelial and Smooth Muscle Progenitor Cells. Int. J. Mol. Sci. 2024, 25, 302. Iordache, F.; Petcu, A.I.; Pisoschi, A.M.; Stanca, L.; Geicu, O.I.; Bilteanu, L.; Curuțiu, C.; Amuzescu, B.; Serban, A.I. PCR Array Profiling of miRNA Expression Involved in the Differentiation of Amniotic Fluid Stem Cells toward Endothelial and Smooth Muscle Progenitor Cells. Int. J. Mol. Sci. 2024, 25, 302.

Abstract

Epigenetic modifications play a significant role in determining the fate of AFSC in terms of differentiation into multiple lineages. There is a tight correlation among DNA methylation, histone modifications, and small noncoding RNAs during the epigenetic control of stem cells differentiation. The present study investigates the role of miRNAs in differentiation of AFSC and addresses how their unique signatures contribute to lineage-specific differentiation. miRNAs profile was assessed in AFSC after 4 weeks of endothelial and muscular differentiation. Our results showed a decreased expression of for 5 miRNAs (miR-125b-5p, hsa-miR-18a-5p, hsa-miR-137, hsa-miR-21-5p and hsa-let-7a-5p) and an increased expression for 12 miRNAs (miR-134-5p, miR-103a-3p, let-7i-5p, miR-214-3p, let-7c-5p, miR-129-5p, miR-210-3p, let-7d-5p, miR-375, miR-181-5p, miR-125a-5p, hsa-let-7e-5p) in EPC compared with undifferentiated AFSC. Smooth muscle differentiation of AFSC revealed significant changes in 9 miRNAs from total of 84 tested; 3 were downregulated (miR-18a-5p, miR-137, sa-miR-21-5p) and 6 were upregulated (miR-155-5p, miR-20a-5p, let-7i-5p, hsa-miR-134-5p, hsa-miR-214-3p, hsa-miR-375). miRNAs are molecular regulators of cell fate and differentiation, recent advances in molecular biology techniques revealed miRNA regulatory networks that can provide us information of endothelial and muscle cell biology in terms of homeostasis, growth, differentiation, and vascular function.

Keywords

microRNA; stem cells; differentiation; endothelial progenitor cells; smooth muscle progenitor cells

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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