Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity

Evgeny A. Zorin
ORCID logo ,
Anton S. Sulima
ORCID logo ,
Aleksandr I. Zhernakov
,
Daria O. Kuzmina
,
Valeria A. Rakova
ORCID logo ,
Marina S. Kliukova
,
Daria A. Romanyuk
,
Olga A. Kulaeva
,
Gulnara A. Akhtemova
,
Oksana Y. Shtark
,
Igor A. Tikhonovich
,
Vladimir A. Zhukov
*
Version 1 : Received: 15 November 2023 / Approved: 15 November 2023 / Online: 15 November 2023 (15:49:19 CET)

A peer-reviewed article of this Preprint also exists.

Zorin, E.A.; Sulima, A.S.; Zhernakov, A.I.; Kuzmina, D.O.; Rakova, V.A.; Kliukova, M.S.; Romanyuk, D.A.; Kulaeva, O.A.; Akhtemova, G.A.; Shtark, O.Y.; Tikhonovich, I.A.; Zhukov, V.A. Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity. Plants 2024, 13, 78. Zorin, E.A.; Sulima, A.S.; Zhernakov, A.I.; Kuzmina, D.O.; Rakova, V.A.; Kliukova, M.S.; Romanyuk, D.A.; Kulaeva, O.A.; Akhtemova, G.A.; Shtark, O.Y.; Tikhonovich, I.A.; Zhukov, V.A. Genomic and Transcriptomic Analysis of Pea (Pisum sativum L.) Breeding Line ‘Triumph’ with High Symbiotic Responsivity. Plants 2024, 13, 78.

Abstract

Pea (Pisum sativum L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype, thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown. Here, we compare the symbiotically responsive breeding line ‘Triumph’ of grain pea with its parental cultivars ‘Vendevil’ (a donor of high symbiotic responsivity) and ‘Classic’ (a donor of agriculturally valuable traits) using genome and transcriptome sequencing. We show that ‘Triumph’ inherited one-fourth of its genome from ‘Vendevil’, including the genes related to AM and nodule formation, and reveal that under combined inoculation with nodule bacteria and AM fungi, ‘Triumph’ and ‘Vendevil’, in contrast to ‘Classic’, demonstrate similar up-regulation of the genes related to solute transport, hormonal regulation and flavonoid biosynthesis in their roots. We also identify the gene PsGLP2, whose expression pattern distinguishing ‘Triumph’ and ‘Vendevil’ from ‘Classic’ correlates with difference within the promoter region sequence, making it a promising marker for the symbiotic responsivity trait. The results of the study may be helpful for future molecular breeding programs aimed at creation of symbiotically responsive cultivars of pea.

Keywords

plant-microbe symbiosis; legumes; Pisum sativum; symbiotic responsivity; genomics; transcriptomics; differential gene expression; molecular breeding

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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