Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Characterisation of Proliferation and Apoptosis of Colonic Epithelium after Electron Irradiation with 2 GY and 25 GY

Grigory Demyashkin
* ORCID logo ,
Elza Karakaeva
,
Susanna Saakian
,
Natalia Tarusova
ORCID logo ,
Amina Guseinova
,
Anita Vays
,
Konstantin Gotovtsev
,
Dmitri Atiakshin
ORCID logo ,
Peter Shegay
,
Andrey D. Kaprin
Version 1 : Received: 12 November 2023 / Approved: 13 November 2023 / Online: 13 November 2023 (10:19:44 CET)

A peer-reviewed article of this Preprint also exists.

Demyashkin, G.; Karakaeva, E.; Saakian, S.; Tarusova, N.; Guseinova, A.; Vays, A.; Gotovtsev, K.; Atiakshin, D.; Shegai, P.; Kaprin, A. Comparative Characterisation of Proliferation and Apoptosis of Colonic Epithelium after Electron Irradiation with 2 GY and 25 GY. International Journal of Molecular Sciences 2024, 25, 1196, doi:10.3390/ijms25021196. Demyashkin, G.; Karakaeva, E.; Saakian, S.; Tarusova, N.; Guseinova, A.; Vays, A.; Gotovtsev, K.; Atiakshin, D.; Shegai, P.; Kaprin, A. Comparative Characterisation of Proliferation and Apoptosis of Colonic Epithelium after Electron Irradiation with 2 GY and 25 GY. International Journal of Molecular Sciences 2024, 25, 1196, doi:10.3390/ijms25021196.

Abstract

Development of new techniques of multimodal treatment, diagnostics of various neoplasms and improvement of these techniques can significantly increase the life expectancy of patients with carcinomas of the colon and abdominal cavity organs, since prevention of various side effects of radiation therapy is one of the main problems of oncological care. Electron irradiation is one of the most promising types of radiation therapy. There are no data on proliferation and apoptosis of the colon epithelium after irradiation with electrons, especially in different modes (single and summary). Aim of the study: Morphological evaluation of apoptosis and proliferation of colonic epithelium after local irradiation with electrons at doses 2 Gy and 25 Gy. Materials and methods. Colon fragments from sexually mature Wistar rats (n=50, body weight 200 ± 10 g) were divided into three groups: I - control (n = 10); II - experimental group (n = 20; local single electron irradiation at a dose of 2 Gy), III - experimental group (n=30), local fractional irradiation with electrons at a total dose of 25 Gy. They were studied by light microscopy using hematoxylin and eosin staining and immunohistochemical reactions with antibodies to Ki-67 and Cas3. Morphological disorders were accompanied by increased expression of pro-apoptotic molecules (caspase-3), and the period of regeneration by proliferative marker (Ki-67). Conclusions: Local fractional irradiation of the colon with electrons at a dose of 2 Gy and 25 Gy resulted in minor changes in the epithelium, a decrease in the number of goblet cells, but with preservation of regenerative potential, mild degenerative-dystrophic lesions. Intestinal crypts were damaged, leading to a decrease in Ki-67 and an increase in the number of caspase-3-positive epitheliocytes.

Keywords

radiotherapy; electrons; irradiation; colon; caspase-3; Ki-67

Subject

Medicine and Pharmacology, Pathology and Pathobiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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