Bychkov, I.A.; Pojidaeva, E.S.; Doroshenko, A.S.; Khripach, V.A.; Kudryakova, N.V.; Kusnetsov, V.V. Phytohormones as Regulators of Mitochondrial Gene Expression in Arabidopsis thaliana. Int. J. Mol. Sci.2023, 24, 16924.
Bychkov, I.A.; Pojidaeva, E.S.; Doroshenko, A.S.; Khripach, V.A.; Kudryakova, N.V.; Kusnetsov, V.V. Phytohormones as Regulators of Mitochondrial Gene Expression in Arabidopsis thaliana. Int. J. Mol. Sci. 2023, 24, 16924.
Bychkov, I.A.; Pojidaeva, E.S.; Doroshenko, A.S.; Khripach, V.A.; Kudryakova, N.V.; Kusnetsov, V.V. Phytohormones as Regulators of Mitochondrial Gene Expression in Arabidopsis thaliana. Int. J. Mol. Sci.2023, 24, 16924.
Bychkov, I.A.; Pojidaeva, E.S.; Doroshenko, A.S.; Khripach, V.A.; Kudryakova, N.V.; Kusnetsov, V.V. Phytohormones as Regulators of Mitochondrial Gene Expression in Arabidopsis thaliana. Int. J. Mol. Sci. 2023, 24, 16924.
Abstract
Coordination of activities between nuclei and organelles in plant cells involves information exchange, in which phytohormones may play an essential role. Therefore, dissection of the mechanisms of hormone-related integration between phytohormones and mitochondria is an important and challenging task. Here, we found that inputs from multiple hormones may cause changes in transcript accumulation of mitochondrial-encoded genes and nuclear genes encoding mitochondrial (mt) proteins. In particular, treatments with exogenous hormones induced changes in GUS expression in the reporter line possessing a 5'-deletion fragment of the RPOTmp promoter. These changes corresponded in part to up- or downregulation of RPOTmp in wild-type plants, which affected the transcription of mt-encoded genes, implying that promoter fragments of the RPOTmp gene are functionally involved in responses to IAA (indole-3-acetic acid), ACC (1-aminocyclopropane-1-carboxylic acid), and ABA (abscisic acid). Hormone-dependent modulations in the expression of mt-encoded genes can also be mediated through mitochondrial transcription termination factors 15, 17, and 18 of the mTERF family and genes for tetratricopeptide repeat proteins that are coexpressed with mTERF genes, in addition to SWIB5 encoding a mitochondrial SWI/SNF (nucleosome remodelling) complex B protein. These genes specifically responded to hormone treatment, displaying both negative and positive regulation in a context-dependent manner. According to bioinformatic resources, their promoter regions possess putative cis-acting elements involved in responses to phytohormones. Alternatively, hormone-related transcriptional activity of these genes may be modulated indirectly, which is especially relevant for brassinosteroids (BS). In general, the results of the study indicate that hormones are essential mediators that are able to cause alterations in the transcript accumulation of mt-related nuclear genes, which in turn trigger the expression of mt genes.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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