Review
Version 1
Preserved in Portico This version is not peer-reviewed
Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine
Version 1
: Received: 3 November 2023 / Approved: 3 November 2023 / Online: 6 November 2023 (10:23:25 CET)
A peer-reviewed article of this Preprint also exists.
Inagaki, M. Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine. J. Dev. Biol. 2024, 12, 1. Inagaki, M. Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine. J. Dev. Biol. 2024, 12, 1.
Abstract
The COVID-19 pandemic stimulated attention to the medicinal applications of messenger RNA (mRNA). mRNA is expected to be applied not only to vaccines but also to regenerative medicines. The purity of mRNA is important for its medicinal application. However, the current mRNA synthesis techniques have problems, e.g., contamination of undesired 5’-uncapped mRNA and double-stranded RNAs. Recently, our group developed a completely capped mRNA synthesis technology that contributed to the progress of mRNA research. The introduction of chemically modified nucleosides, e.g., N1-methylpseudouridine and 5-methylcytidine, have been demonstrated by Dr. Karikó and Dr. Weissman which opened the practical application of mRNA for vaccines and regenerative medicines. Dr. Yamanaka reported the production of Induced Pluripotent Stem Cells (iPSCs) by the introduction of 4 types of genes using a retrovirus vector. iPSCs are widely used for research about regenerative medicines and the preparation of disease models to screen new drug candidates. Of the Yamanaka factors, Klf4 and c-Myc are oncogenes, and there is a tumor development risk if these are integrated into genomic DNA. Therefore, regenerative medicines using mRNA, which has no risk of genome insertion, have attracted attention. In this review, we summarized the synthesis of mRNA and its application for regenerative medicine.
Keywords
Messenger RNA; Cell Regeneration; Pluripotent Cells; Cellular Differentiation, Direct Reprogramming, Regenerative Medicine
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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