Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Physicochemical Characteristics and Antimicrobial Efficacy of Plasma-Activated Water Produced by an Air-Operated Coaxial DBD Plasma

Version 1 : Received: 2 November 2023 / Approved: 3 November 2023 / Online: 3 November 2023 (10:14:16 CET)

A peer-reviewed article of this Preprint also exists.

Miranda, F.S.; Tavares, V.K.F.; Gomes, M.P.; Neto, N.F.A.; Chiappim, W.; Petraconi, G.; Pessoa, R.S.; Koga-Ito, C.Y. Physicochemical Characteristics and Antimicrobial Efficacy of Plasma-Activated Water Produced by an Air-Operated Coaxial Dielectric Barrier Discharge Plasma. Water 2023, 15, 4045. Miranda, F.S.; Tavares, V.K.F.; Gomes, M.P.; Neto, N.F.A.; Chiappim, W.; Petraconi, G.; Pessoa, R.S.; Koga-Ito, C.Y. Physicochemical Characteristics and Antimicrobial Efficacy of Plasma-Activated Water Produced by an Air-Operated Coaxial Dielectric Barrier Discharge Plasma. Water 2023, 15, 4045.

Abstract

In this study, Plasma-Activated Water (PAW) was synthesized using a coaxial Dielectric Barrier Discharge (DBD) reactor, benefiting from the elevated capacity of air-flow-assisted DBD discharges to enhance nitrogen-based species concentration. By manipulating operational parameters, including gas flow rate, activation time, and DI water volume, we achieved significant concentrations of Reactive Oxygen and Nitrogen Species (RONS). As a result, the PAW obtained displayed pronounced physicochemical attributes: a pH of 2.06, an ORP of 275mV, conductivity of 3mS/cm, and TDS of 1200 mg/L. A pivotal aspect of this research was the evaluation of the reactor's efficiency, as indicated by metrics like the specific input energy and ozone efficiency yield. The antimicrobial potential of the PAW was also assessed against pathogenic microbes, with remarkable reductions in viability for both Staphylococcus aureus and Escherichia coli (99.99%) and a more moderate decrease for Candida albicans (37%). These findings underscore the capability of the coaxial DBD reactor in crafting high-quality PAW with significant antimicrobial properties, necessitating further studies to validate its broad-spectrum and safe application.

Keywords

Cold atmospheric plasma; Dielectric Barrier Discharge; Plasma-activated liquids; Plasma medi-cine; Staphylococcus aureus; Escherichia coli; Candida albicans

Subject

Environmental and Earth Sciences, Water Science and Technology

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