CRISPR as Genome Editing Technique in Fruit Trees Breeding

Marina Martín-Valmaseda; Sama Rahimi Devin; Germán Ortuño-Hernández; Cristian Pérez-Caselles; Sayyed Mohammad Ehsan Mahdavi; Geza Bujdoso; Juan Alfonso Salazar; Pedro Martínez-Gómez; Nuria Alburquerque

doi:10.20944/preprints202311.0143.v1

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preprints.org > biology and life sciences > plant sciences > doi: 10.20944/preprints202311.0143.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

CRISPR as Genome Editing Technique in Fruit Trees Breeding

Marina Martín-Valmaseda

Sama Rahimi Devin

Germán Ortuño-Hernández

Cristian Pérez-Caselles

Sayyed Mohammad Ehsan Mahdavi

^* ,

Version 1 : Received: 2 November 2023 / Approved: 2 November 2023 / Online: 2 November 2023 (10:11:14 CET)

A peer-reviewed article of this Preprint also exists.

Martín-Valmaseda, M.; Devin, S.R.; Ortuño-Hernández, G.; Pérez-Caselles, C.; Mahdavi, S.M.E.; Bujdoso, G.; Salazar, J.A.; Martínez-Gómez, P.; Alburquerque, N. CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding. Int. J. Mol. Sci. 2023, 24, 16656. Martín-Valmaseda, M.; Devin, S.R.; Ortuño-Hernández, G.; Pérez-Caselles, C.; Mahdavi, S.M.E.; Bujdoso, G.; Salazar, J.A.; Martínez-Gómez, P.; Alburquerque, N. CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding. Int. J. Mol. Sci. 2023, 24, 16656.

Abstract

The methods used to introduce CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out of target genes in different fruit trees species including tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses have been firstly described in this review. The application of this gene editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits in elite cultivars without changing other traits. However, at this moment, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are the genotype-dependent or the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, latest news on the legislation and regulations about the use of plants modified through CRISPR/Cas systems has been also discussed.

Keywords

Plants; Molecular Biology; Genomic; Transcriptomic; Epigenetic

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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