Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Unexpected Differences in the Speed of Non-malignant versus Malignant Cell Migration Reveal Differential Basal ATP Levels

Bareun Kim
ORCID logo ,
Anthony T. Lopez
,
Indhujah Thevarajan
ORCID logo ,
Maria F. Osuna
,
Monica Mallavarapu
,
Boning Gao
,
Jihan K. Osborne
* ORCID logo
Version 1 : Received: 1 November 2023 / Approved: 1 November 2023 / Online: 1 November 2023 (11:18:02 CET)

A peer-reviewed article of this Preprint also exists.

Kim, B.; Lopez, A.T.; Thevarajan, I.; Osuna, M.F.; Mallavarapu, M.; Gao, B.; Osborne, J.K. Unexpected Differences in the Speed of Non-Malignant versus Malignant Cell Migration Reveal Differential Basal Intracellular ATP Levels. Cancers 2023, 15, 5519. Kim, B.; Lopez, A.T.; Thevarajan, I.; Osuna, M.F.; Mallavarapu, M.; Gao, B.; Osborne, J.K. Unexpected Differences in the Speed of Non-Malignant versus Malignant Cell Migration Reveal Differential Basal Intracellular ATP Levels. Cancers 2023, 15, 5519.

Abstract

Cellular locomotion is required for survival, fertility, proper embryonic development, regeneration, and wound healing. Cell migration is a major component of metastasis, which accounts for two-thirds of all solid tumor deaths. While many studies have demonstrated increased energy requirements, metabolic rates, and migration of cancer cells compared to normal cells, few have systematically compared normal and cancer cell migration as well as energy requirements side by side. Thus, we investigated how non-malignant and malignant cells migrate utilizing several cell lines from the breast and lung. Initial screening was done in an unbiased high-throughput manner for the ability to migrate/invade on collagen and/or Matrigel. We unexpectedly observed that all the non-malignant lung cells moved significantly faster than cells derived from lung tumors regardless of growth media used. Given the paradigm-shifting nature of our discovery, we pursued possible mechanisms responsible. Neither mass, cell doubling, nor volume, accounted for the individual speed and track length of the normal cells. Non-malignant cells had higher levels of ATP at premigratory-wound induction stages. Meanwhile, cancer cells also increased ATP at premigratory-wound induction – but not to the levels of the normal cells, indicating the possibility for further therapeutic investigation.

Keywords

Cell Migration; Cancer cell migration; normal epithelial cell motility

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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