Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Assessment of Microbiological Quality of Water Using Culture Methods, Flow Cytometry and Luminometry

Version 1 : Received: 25 October 2023 / Approved: 25 October 2023 / Online: 25 October 2023 (11:51:15 CEST)

A peer-reviewed article of this Preprint also exists.

Zamorska, J.; Karwowska, E.; Przystaś, W. Assessment of Microbiological Quality of Water Using Culture Methods, Flow Cytometry and Luminometry. Water 2023, 15, 4077. Zamorska, J.; Karwowska, E.; Przystaś, W. Assessment of Microbiological Quality of Water Using Culture Methods, Flow Cytometry and Luminometry. Water 2023, 15, 4077.

Abstract

A very important role in determining the quality of water is the assessment of its microbiological quality. Water quality control, which could pose a direct threat to human health and life, is performed in the case of water produced at the water treatment plants, tap water or water in swimming pools. However, these traditional methods used to assess its quality are laborious and time consuming. In emergency and incidental situations, in the era of terrorist threats, the need for quick, reliable and reproducible microbiological determinations seems to be essential. In this study, an attempt was made to compare various methods of assessing the microbiological quality of water. The assessment was carried out for waters with different microbiological characteristics: surface waters, rainwater, groundwater, and water supply. The evaluation was carried out using traditional culture methods and high-speed methods: flow cytometry and luminometry. The analysis of microbiological parameters were the basis for the statistical analysis. The conducted microbiological analysis of various types of water, along with their statistical evaluation, showed different dependencies for each of the analysed waters.

Keywords

microbiology of water; water quality assessment; culture methods; flow cytometry; luminometry

Subject

Environmental and Earth Sciences, Environmental Science

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