preprints.org > biology and life sciences > neuroscience and neurology > doi: 10.20944/preprints202310.1188.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 16 October 2023 / Approved: 18 October 2023 / Online: 19 October 2023 (03:28:44 CEST)

A wide variety of substances have been used to anaesthetise invertebrates, but many are not anaesthetics and merely incapacitate animals, rather than preventing pain. Much is now known about the mode of action of modern clinical and veterinary anaesthetics because of their use on human beings and other vertebrates. In essence, the role of an ideal general anaesthetic is to act as a muscle relaxant, an analgesic, an anaesthetic and an amnesic. To achieve all these properties with a single substance is difficult and various adjuvants usually need to be administered, resulting in a cocktail of drugs. In a clinical setting, the vast majority of patients are unaware of surgery being carried out and have no memory of it, so they can claim to have felt no pain, but this is much more difficult to demonstrate in invertebrates. Here we show that 1% MgCl2, a muscle relaxant, is a useful adjuvant for the clinical anaesthetic isoflurane on Octopus vulgaris when applied for 10 minutes before the clinical anaesthetic. After this, full anaesthesia can be achieved in 5 minutes using only 1% isoflurane and from which full recovery takes place rapidly in about 10 to 15 minutes. This reduces stress on the animal and minimises the quantity of anaesthetic used.

invertebrate welfare; Octopus vulgaris; Anaesthetic protocol; Isoflurane; Magnesium chloride; stress reduction

Biology and Life Sciences, Neuroscience and Neurology

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