Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

MethylRAD-Seq Technology Reveals DNA Methylation Charac-Teristics of Apostichopus japonicus of Different Ages

Version 1 : Received: 16 October 2023 / Approved: 17 October 2023 / Online: 18 October 2023 (08:21:09 CEST)

A peer-reviewed article of this Preprint also exists.

Yang, X.; Han, L.; Ye, Q.; Wang, H.; Zhang, J.; Wang, W.; Xiao, H.; Wang, Y.; Wang, L.; Ding, J. MethylRAD Sequencing Technology Reveals DNA Methylation Characteristics of Apostichopus japonicus of Different Ages. Animals 2023, 13, 3530. Yang, X.; Han, L.; Ye, Q.; Wang, H.; Zhang, J.; Wang, W.; Xiao, H.; Wang, Y.; Wang, L.; Ding, J. MethylRAD Sequencing Technology Reveals DNA Methylation Characteristics of Apostichopus japonicus of Different Ages. Animals 2023, 13, 3530.

Abstract

The A. japonicus industry has expanded significantly, but no research has focused on how to de-termine the age of A. japonicus during farming. Correctly estimating the age of A. japonicus can provide a decision-making basis for the breeding process, and data for the protection of A. japonicus aquatic germplasm resources. DNA methylation levels in the body wall of Apostichopus japonicus at 4 months, 1 year, 2 years, and 3 years old were determined by MethylRAD-Seq, and differentially methylated genes related to age were screened. The results of the study found that 441 and 966 differentially methylated genes were detected at CCGG and CCWGG sites, respectively. As-partate aminotransferase, succinate semialdehyde dehydrogenase, isocitrate dehydrogenase, the histone H2AX, heat shock protein Hsp90, aminopeptidase N, cell division cycle CDC6, Ras GTPase activating protein (RasGAP), slit guidance ligand slit 1, integrin linked kinase ILK, mechanistic target of rapamycin kinase Mtor, protein kinase A Pka, and autophagy-related 3 atg3 these genes may play key roles in the growth and aging process of A. japonicus. This study provided data for identifying the age of A. japonicus.

Keywords

MethylRAD-Seq; A. japonicus; body wall; age identification

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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