Version 1
: Received: 9 October 2023 / Approved: 10 October 2023 / Online: 10 October 2023 (10:06:54 CEST)
How to cite:
Auteleyeva, L.; Maikanov, B.; Akylbekov, N. A New Method for Extraction of Aflatoxin B1 from Nuts for Competitive Enzyme-linked Immuno Assay. Preprints2023, 2023100622. https://doi.org/10.20944/preprints202310.0622.v1
Auteleyeva, L.; Maikanov, B.; Akylbekov, N. A New Method for Extraction of Aflatoxin B1 from Nuts for Competitive Enzyme-linked Immuno Assay. Preprints 2023, 2023100622. https://doi.org/10.20944/preprints202310.0622.v1
Auteleyeva, L.; Maikanov, B.; Akylbekov, N. A New Method for Extraction of Aflatoxin B1 from Nuts for Competitive Enzyme-linked Immuno Assay. Preprints2023, 2023100622. https://doi.org/10.20944/preprints202310.0622.v1
APA Style
Auteleyeva, L., Maikanov, B., & Akylbekov, N. (2023). A New Method for Extraction of Aflatoxin B1 from Nuts for Competitive Enzyme-linked Immuno Assay. Preprints. https://doi.org/10.20944/preprints202310.0622.v1
Chicago/Turabian Style
Auteleyeva, L., Balgabay Maikanov and Nurgali Akylbekov. 2023 "A New Method for Extraction of Aflatoxin B1 from Nuts for Competitive Enzyme-linked Immuno Assay" Preprints. https://doi.org/10.20944/preprints202310.0622.v1
Abstract
Nuts are a staple food consumed throughout the world, and their purity is essential to maintaining human health. However, fungal mycotoxins pose one of the real challenges in keeping nuts clean for human consumption. The high cost of equipment, reagents used, standard samples, as well as increased requirements for personnel qualifications limit the use of chromatographic methods in testing laboratories, both manufacturing enterprises and organizations monitoring food safety. The present study aimed to test a new method for extracting aflatoxin B1 using sodium chloride and citric acid instead of methanol present in imported nuts obtained from local markets in Astana (Kazakhstan). The proposed method for extracting aflatoxin B1 from nuts is chemically safe, since the substances used belong to hazard class 3 of the international classification and do not require separate premises in food safety laboratories of the Astana markets and special training of specialists. The technical objective of the method is to replace the reagent (methanol) during the sample preparation process when conducting cocuret enzyme-linked immunosorbent assay. Aflatoxin B1 levels varied depending on the type of nut. Aflatoxin contamination was observed in 35.8% of samples analyzed. These levels of mycotoxin contamination are alarming and raise serious questions about the purity of imported nuts to ensure food safety.
Keywords
Aflatoxin B1; mycotoxins; various nuts; contamination; food safety; extraction
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
