Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Daptomycin-Impregnated PMMA Cement against Vancomycin-Resistant Germs: Dosage, Handling, Elution, Mechanical Stability, and Effectiveness

Martina Humez
* ,
Eugen Domann
ORCID logo ,
Kai Thormann
,
Christian Foelsch
,
Rainer Strathausen
,
Sebastian Vogt
,
Volker Alt
,
Klaus-Dieter Kühn
*
Version 1 : Received: 9 October 2023 / Approved: 10 October 2023 / Online: 10 October 2023 (07:17:42 CEST)

A peer-reviewed article of this Preprint also exists.

Humez, M.; Domann, E.; Thormann, K.M.; Fölsch, C.; Strathausen, R.; Vogt, S.; Alt, V.; Kühn, K.-D. Daptomycin-Impregnated PMMA Cement against Vancomycin-Resistant Germs: Dosage, Handling, Elution, Mechanical Stability, and Effectiveness. Antibiotics 2023, 12, 1567. Humez, M.; Domann, E.; Thormann, K.M.; Fölsch, C.; Strathausen, R.; Vogt, S.; Alt, V.; Kühn, K.-D. Daptomycin-Impregnated PMMA Cement against Vancomycin-Resistant Germs: Dosage, Handling, Elution, Mechanical Stability, and Effectiveness. Antibiotics 2023, 12, 1567.

Abstract

Background: Number of joint infections caused by Vancomycin-resistant pathogens is emerging. Currently no PMMA cement is commercially available to cover VRE. Daptomycin shows promising results in infection treatment, offers a good safety profile and reduced risk for resistance development. Purpose of this in vitro study is to investigate the mechanical stability, handling properties, elution behavior and antimicrobial effectiveness of PMMA cement loaded with three different Daptomycin concentrations in comparison to commercially available ALBC; Methods: Mechanical properties and handling characteristics (ISO 5833, DIN 53435), HPLC elution, antimicrobial effectiveness with proliferation assay (DIN 17025) and inhibition zone testing were investigated; Results: All tested Daptomycin concentrations fulfilled ISO and DIN mechanical strength. Dosage of 0.5. g added Daptomycin did not show any antimicrobial effectiveness in contrast to 1.0 g and1.5 g. Cement with 1.5 g Daptomycin was best in elution and effectiveness, showed good mechanical ISO strength, ISO doughing was a little longer sticky and setting was faster as Vancomycin containing reference cement; Conclusion: PMMA cement containing 0.5 g Gentamicin and 1.5 g Daptomycin could be a good alternative to the already established COPAL® G+V for PJI treatment of VRE.

Keywords

Daptomycin; PMMA cement; mechanical properties; elution; antimicrobial effectiveness; inhibition zone test; Vancomycin-resistant germs; PMMA spacer

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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