Ouchida, T.; Tanaka, T.; Suzuki, H.; Uchida, K.; Nakagawa, T.; Li, G.; Nakamura, T.; Yanaka, M.; Handa, S.; Kaneko, M.K.; et al. PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2023, doi:10.1089/mab.2023.0020.
Ouchida, T.; Tanaka, T.; Suzuki, H.; Uchida, K.; Nakagawa, T.; Li, G.; Nakamura, T.; Yanaka, M.; Handa, S.; Kaneko, M.K.; et al. PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2023, doi:10.1089/mab.2023.0020.
Ouchida, T.; Tanaka, T.; Suzuki, H.; Uchida, K.; Nakagawa, T.; Li, G.; Nakamura, T.; Yanaka, M.; Handa, S.; Kaneko, M.K.; et al. PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2023, doi:10.1089/mab.2023.0020.
Ouchida, T.; Tanaka, T.; Suzuki, H.; Uchida, K.; Nakagawa, T.; Li, G.; Nakamura, T.; Yanaka, M.; Handa, S.; Kaneko, M.K.; et al. PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2023, doi:10.1089/mab.2023.0020.
Abstract
Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.
Keywords
podoplanin; PDPN; giraffe; Immunohistochemistry
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
