Romeo, F.; Delgado, S.; Yavorsky, M.; Martinez Cuesta, L.; Pereyra, S.; González Altamiranda, E.; Louge Uriarte, E.; Pérez, S.; Verna, A. Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process. Biology2024, 13, 249.
Romeo, F.; Delgado, S.; Yavorsky, M.; Martinez Cuesta, L.; Pereyra, S.; González Altamiranda, E.; Louge Uriarte, E.; Pérez, S.; Verna, A. Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process. Biology 2024, 13, 249.
Romeo, F.; Delgado, S.; Yavorsky, M.; Martinez Cuesta, L.; Pereyra, S.; González Altamiranda, E.; Louge Uriarte, E.; Pérez, S.; Verna, A. Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process. Biology2024, 13, 249.
Romeo, F.; Delgado, S.; Yavorsky, M.; Martinez Cuesta, L.; Pereyra, S.; González Altamiranda, E.; Louge Uriarte, E.; Pérez, S.; Verna, A. Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process. Biology 2024, 13, 249.
Abstract
The prevalent pathogens associated with bovine uterine infections are bacteria that appear to increase the host's susceptibility to secondary infections with other bacteria or viruses, among which BoHV-4 is the most frequently found. In this work, the study of the pathways of apoptosis induction was carried out on an experimental model of primary culture of endometrial cells, in order to know the implication of BoHV-4 and the presence of bacterial LPS in the pathogenesis of the bovine reproductive tract. For this, different staining techniques and molecular analysis by RT-PCR were used. The results obtained allowed us to conclude that the level of cell death observed in the proposed primary culture is directly related to the time of viral infection and the presence of LPS in BoHV-4 infection. The apoptosis indices in cells infected with BoHV-4, BoHV-4 + LPS presented a maximum that correlates with the appearance of the cytopathic effect, and the maximum viral titers in the model studied. However, morphological, biochemical and molecular changes were evident during both early and late stages of apoptosis. These findings provide information on the factors that may influence the pathogenesis of BoHV-4 and help to better understand the mechanisms involved in virus infection.
Keywords
Bovine herpesvirus type 4; bovine endometrial primary culture cells; apoptosis
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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