Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Members Expression

Estefanía Lozano-Velasco
ORCID logo ,
Carlos Garcia-Padilla
,
Miguel Carmona-Garcia
,
Alba Gonzalez-Diaz
,
Angela Arequipa-Rendon
,
Amelia Eva Aránega
ORCID logo ,
Diego Franco
* ORCID logo
Version 1 : Received: 4 October 2023 / Approved: 5 October 2023 / Online: 6 October 2023 (05:46:11 CEST)

How to cite: Lozano-Velasco, E.; Garcia-Padilla, C.; Carmona-Garcia, M.; Gonzalez-Diaz, A.; Arequipa-Rendon, A.; Aránega, A.E.; Franco, D. MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Members Expression. Preprints 2023, 2023100287. https://doi.org/10.20944/preprints202310.0287.v1 Lozano-Velasco, E.; Garcia-Padilla, C.; Carmona-Garcia, M.; Gonzalez-Diaz, A.; Arequipa-Rendon, A.; Aránega, A.E.; Franco, D. MEF2C Directly Interacts with Pre-miRNAs and Distinct RNPs to Post-transcriptionally Regulate miR-23a-miR-27a-miR-24-2 microRNA Cluster Members Expression. Preprints 2023, 2023100287. https://doi.org/10.20944/preprints202310.0287.v1

Abstract

Transcriptional regulation constitutes a key step in gene expression regulation. Myocyte enhancer factor 2C (MEF2C) is a transcription factor of MADS box family involved in the early development of several cell types including muscle cells. Over the last decade a novel layer of complexity modulating gene regulation is emerging as non-coding RNAs have been identified, impacting in both transcriptional and post-transcriptional regulation. microRNAs represent the most studied and abundantly expressed subtype of small non-coding RNAs and their functional role have been widely documented. On the other hand, our knowledge on the transcriptional and post-transcriptional regulatory mechanisms that drive microRNA expression is still incipient. We recently demonstrated that MEF2C is able to transactive the long, but not short, regulatory element upstream of miR-23a-miR-27a-miR-24-2 transcriptional start site. However, MEF2C over-expression and silencing, respectively, displayed distinct effects on each of the miR-23a-miR-27a-miR-24-2 mature cluster members, without affecting pri-miRNA expression levels, supporting thus additional MEF2C-driven regulatory mechanisms. Within this study we demonstrated a complex post-transcriptional regulatory mechanisms directed by MEF2C in the regulation of miR-23a-miR-27a-miR-24-2 cluster members, distinctly involving different domains of the MEF2C transcription factor and the physical interaction with pre-miRNAs and RNA interacting proteins such as Ksrp, HnRNPa3 and Ddx17.

Keywords

Mef2c; microRNAs; RNPs

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.