Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Characterization of Native Strains of Bacillus spp. in rhizospheric Soil in Three Regions of Peru

Version 1 : Received: 5 October 2023 / Approved: 5 October 2023 / Online: 5 October 2023 (07:54:35 CEST)

How to cite: Quispe, A.; Contreras, M.; Meza, D.; Vicente, J. Molecular Characterization of Native Strains of Bacillus spp. in rhizospheric Soil in Three Regions of Peru. Preprints 2023, 2023100253. https://doi.org/10.20944/preprints202310.0253.v1 Quispe, A.; Contreras, M.; Meza, D.; Vicente, J. Molecular Characterization of Native Strains of Bacillus spp. in rhizospheric Soil in Three Regions of Peru. Preprints 2023, 2023100253. https://doi.org/10.20944/preprints202310.0253.v1

Abstract

Abrstract: Bacillus spp. it is a genus that is used to biocontrol post-harvest diseases in various vegetables and fruits during transportation and storage. It suppresses the development of pathogens such as gray mold (Botrytis cinerea) in strawberry cultivation. In this work, the molecular characterization of Bacillus spp. species was carried out. in three cities in Peru: Huaura, Chanchamayo and Cañete. The samples were collected from rhizospheric soil from crop areas, subsequently processed for microbiological isolation, molecular analysis using the 16S rRNA gene and bioinformatic analysis. In total, 14 strains were obtained where they were identified with a minimum of 92.05% and a maximum of 100% similarity: 8 strains of Bacillus subtilis, 4 strains of Bacillus licheniformis, 1 strain of Bacillus paralicheniformis and 1 strain of Bacillus cereus, all the strains were related by constructing a phylogenetic tree.

Keywords

Bacillus; 16S rRNA; molecular characterization; rhizosphere; biocontroller

Subject

Biology and Life Sciences, Biology and Biotechnology

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