Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Early Castration in Horses Does Not Impact Osteoarticular Metabolism

Marion Rouge
,
Florence Legendre
ORCID logo ,
Razan Elkhatib
,
Christelle Delalande
,
Juliette Cognié
,
Fabrice Reigner
,
Philippe Barrière
,
Stefan Deleuze
ORCID logo ,
Vincent Hanoux
,
Philippe Galéra
ORCID logo ,
Hélène Bouraïma-Lelong
*
Version 1 : Received: 28 September 2023 / Approved: 29 September 2023 / Online: 29 September 2023 (05:25:34 CEST)

A peer-reviewed article of this Preprint also exists.

Rouge, M.; Legendre, F.; Elkhatib, R.; Delalande, C.; Cognié, J.; Reigner, F.; Barrière, P.; Deleuze, S.; Hanoux, V.; Galéra, P.; Bouraïma-Lelong, H. Early Castration in Horses Does Not Impact Osteoarticular Metabolism. Int. J. Mol. Sci. 2023, 24, 16778. Rouge, M.; Legendre, F.; Elkhatib, R.; Delalande, C.; Cognié, J.; Reigner, F.; Barrière, P.; Deleuze, S.; Hanoux, V.; Galéra, P.; Bouraïma-Lelong, H. Early Castration in Horses Does Not Impact Osteoarticular Metabolism. Int. J. Mol. Sci. 2023, 24, 16778.

Abstract

Castration of stallions is traditionally performed after puberty around the age of 2 years old. No studies have focused on the effects of early castration on osteoarticular metabolism. Thus, we sought to compare early castration (3 days after birth) with traditional castration (18 months of age) in horses. Testosterone and estradiol levels were monitored from birth to 33 months in these two groups. We quantified the levels of biomarkers of cartilage and bone anabolism (CPII and N-MID) and catabolism (CTX-I and CTX-II), of osteoarthritis (HA and COMP) and inflammation (IL-6 and PGE2). We revealed a lack of parallelism between testosterone and estradiol syntheses after birth and during puberty in both groups. An extra-gonadal synthesis of steroids was observed around the 28 month-mark, regardless of the castration age. We found the expression of estrogen receptor (ESR1) in cartilage and bone, whereas androgen receptor (AR) expression appeared to be restricted to bone. Nevertheless, with regards to osteoarticular metabolism, steroid hormone deprivation resulting from early castration showed no discernable impact on the levels of biomarkers related to bone and cartilage metabolism, nor on those associated with OA and inflammation. Consequently, our research demonstrated that early castration does not disrupt bone and cartilage homeostasis.

Keywords

horse; castration; steroid hormones; cartilage; bone; in vivo metabolism

Subject

Biology and Life Sciences, Endocrinology and Metabolism

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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