Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Snapshot of the Phylogenetic Relationships among Avian Pox-Viruses Circulating in Portugal between 2017 and 2023

Daniela Santos
,
Teresa Fagulha
ORCID logo ,
Margarida Dias Duarte
ORCID logo ,
Ana Duarte
,
Fernanda Ramos
,
Sílvia C Barros
ORCID logo ,
Tiago Luís
,
Ana Margarida Henriques
* ORCID logo
Version 1 : Received: 27 September 2023 / Approved: 28 September 2023 / Online: 29 September 2023 (08:32:26 CEST)

A peer-reviewed article of this Preprint also exists.

Santos, D.; Fagulha, T.; Duarte, M.D.; Duarte, A.; Ramos, F.; Barros, S.C.; Luís, T.; Henriques, A.M. Snapshot of the Phylogenetic Relationships among Avian Poxviruses Circulating in Portugal between 2017 and 2023. Vet. Sci. 2023, 10, 693. Santos, D.; Fagulha, T.; Duarte, M.D.; Duarte, A.; Ramos, F.; Barros, S.C.; Luís, T.; Henriques, A.M. Snapshot of the Phylogenetic Relationships among Avian Poxviruses Circulating in Portugal between 2017 and 2023. Vet. Sci. 2023, 10, 693.

Abstract

Avipoxvirus (APV), a linear dsDNA virus belonging to the subfamily Chordopoxvirinae of the family Poxviridae, infects more than 278 species of domestic and wild birds. It is responsible for causing the avian pox disease, which is characterized by its cutaneous and diphtheric forms. With a high transmission capacity, it can cause high economic losses and damage to the ecosys-tem. Several diagnostic methods are available and vaccination of birds can be an effective pre-ventive measure. To update the molecular characterization and phylogenetic analysis of viruses isolated in Portugal between 2017 and 2023, ten APV-positive samples were analyzed. A P4b gene fragment was amplified by PCR and the nucleotide sequence of the amplicons was deter-mined by Sanger sequencing. The sequences obtained were aligned using ClustalW, and a max-imum likelihood phylogenetic tree was constructed. With this study, it was possible to verify that the analyzed sequences are distributed in subclades A1, A2, B1, and B3. Since some of them are quite similar to others from different countries and obtained in different years, it is possible to conclude that there have been several viral introductions in Portugal. Finally, it was possible to successfully update the data on avipoxviruses in Portugal.

Keywords

Avipoxvirus; molecular characterization; phylogenetic analysis; viral introduction; Portugal

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.