Dantas, L.R.; Kraft, L.; Gonçalves, G.A.; Ribeiro, V.S.T.; Witt, M.A.; Carneiro, E.; Tuon, F.F. Antimicrobial Activity of Bone Scaffolds Impregnated with Silver Nanoparticles on Bacterial and Fungal Biofilm. Preprints2023, 2023091948. https://doi.org/10.20944/preprints202309.1948.v1
APA Style
Dantas, L.R., Kraft, L., Gonçalves, G.A., Ribeiro, V.S.T., Witt, M.A., Carneiro, E., & Tuon, F.F. (2023). Antimicrobial Activity of Bone Scaffolds Impregnated with Silver Nanoparticles on Bacterial and Fungal Biofilm. Preprints. https://doi.org/10.20944/preprints202309.1948.v1
Chicago/Turabian Style
Dantas, L.R., Everdan Carneiro and Felipe Francisco Tuon. 2023 "Antimicrobial Activity of Bone Scaffolds Impregnated with Silver Nanoparticles on Bacterial and Fungal Biofilm" Preprints. https://doi.org/10.20944/preprints202309.1948.v1
Abstract
Introduction: Silver nanoparticles have been extensively investigated in dental and orthopedic materials. However, the impregnation of bone graft with silver nanoparticles has been poorly investigated. Objectives: The aim of this study was to evaluate the in vitro antibiofilm activity of a bovine bone impregnated with silver nanoparticles. Methods: Bone scaffolds from cancellous bovine femur were used for the tests and impregnated with silver nanoparticles (50nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentration were performed Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis, Acinetobacter baumannii, and Escherichia coli. Disc diffusion tests for silver nanoparticles susceptibility and quantification of biofilm production on plate and bone with sessile cell count were also performed. Results: All pathogens were susceptible to silver with low minimal inhibitory concentration (0.25 – 4 mg/L). The scaffold impregnated with silver nanoparticles presented a significant reduction in the biofilm cells for all microorganisms with a reduction of more than 3 logs in colony forming units count. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce biofilm, and it can be a strategical material to be used as an implant for different approaches.
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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