Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Longitudinal Analysis of Neuraminidase Antibodies to Influenza a Viruses after Immunization with Seasonal Inactivated Influenza Vaccines

Mariia V. Sergeeva
ORCID logo ,
E Romanovskaya-Romanko
,
V Krivitskaya
,
Polina Kudar
ORCID logo ,
N Petkova
,
K Kudrya
,
D Lioznov
ORCID logo ,
M Stukova
,
Y Desheva
* ORCID logo
Version 1 : Received: 26 September 2023 / Approved: 27 September 2023 / Online: 28 September 2023 (17:59:45 CEST)

A peer-reviewed article of this Preprint also exists.

Sergeeva, M.V.; Romanovskaya-Romanko, E.A.; Krivitskaya, V.Z.; Kudar, P.A.; Petkova, N.N.; Kudria, K.S.; Lioznov, D.A.; Stukova, M.A.; Desheva, Y.A. Longitudinal Analysis of Neuraminidase and Hemagglutinin Antibodies to Influenza A Viruses after Immunization with Seasonal Inactivated Influenza Vaccines. Vaccines 2023, 11, 1731. Sergeeva, M.V.; Romanovskaya-Romanko, E.A.; Krivitskaya, V.Z.; Kudar, P.A.; Petkova, N.N.; Kudria, K.S.; Lioznov, D.A.; Stukova, M.A.; Desheva, Y.A. Longitudinal Analysis of Neuraminidase and Hemagglutinin Antibodies to Influenza A Viruses after Immunization with Seasonal Inactivated Influenza Vaccines. Vaccines 2023, 11, 1731.

Abstract

Neuraminidase (NA)-based immunity could reduce the harmful impact of novel antigenic variants of influenza viruses. Detection of neuraminidase-inhibiting (NI) antibodies in parallel with anti-hemagglutinin (HA) antibodies may enhance research of immunogenicity and duration of antibody response to influenza vaccines. To assess anti-NA antibodies after vaccination with seasonal inactivated influenza vaccines, we used the enzyme-linked lectin assay, and anti-HA antibodies were detected in hemagglutination inhibition assay. The dynamics of anti-NA antibody response differed depending on the virus subtype: antibodies to A/H3N2 virus neuraminidase increased later than antibodies to A/H1N1pdm09 subtype neuraminidase, and persisted longer. In contrast to HA antibodies, the fold increase in antibody titers to NA after vaccination poorly depended on the preexisting level. At the same time, NA antibody level after vaccination directly correlated with titers before vaccination. Difference was found in response to NA antigen between split and subunit adjuvanted vaccines and in NA functional activity in the vaccine formulations.

Keywords

influenza; vaccines; antibodies; neuraminidase-inhibition; persistence.

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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