Lim, D.W.; Yoon, T.-S.; Han, K.H.; Sajjad, S.; Shin, H.-S.; Kang, S. Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module. Methods Protoc.2023, 6, 106.
Lim, D.W.; Yoon, T.-S.; Han, K.H.; Sajjad, S.; Shin, H.-S.; Kang, S. Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module. Methods Protoc. 2023, 6, 106.
Lim, D.W.; Yoon, T.-S.; Han, K.H.; Sajjad, S.; Shin, H.-S.; Kang, S. Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module. Methods Protoc.2023, 6, 106.
Lim, D.W.; Yoon, T.-S.; Han, K.H.; Sajjad, S.; Shin, H.-S.; Kang, S. Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module. Methods Protoc. 2023, 6, 106.
Abstract
The horizontal flatbed electrophoresis system has been used for the electrophoresis of protein samples and is often preferred for a two-dimensional polyacrylamide gel electrophoresis. In currently available equipment, cathode and anode electrodes were placed on top of a gel at each end. An electric field entered from the top of a gel, and its strength was relatively weaker in the bottom part of the gel. Such configuration caused migrating protein bands to lie down forward in the migrating direction. New electrodes, i.e., the clamp-shaped and the double-deck electrodes, were developed to simultaneously apply an electric field from the top and bottom of a gel. Electrophoretic separation with these electrodes made protein bands more focused and improved protein band resolutions. When the FIGE technique was tested together with new electrodes, protein bands were aligned nearly vertically and showed the best electrophoretic resolutions.
Keywords
Horizontal PAGE; Electrode; FIGE
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
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