Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Surface Morphology and Human Osteoblast Response of 316L Stainless Steel after Various Surface Treatments

Version 1 : Received: 25 September 2023 / Approved: 26 September 2023 / Online: 27 September 2023 (04:15:55 CEST)

A peer-reviewed article of this Preprint also exists.

Hsiao, V.K.S.; Lin, Y.-C.; Wu, H.-C.; Wu, T.-I. Surface Morphology and Human MG-63 Osteoblasic Cell Line Response of 316L Stainless Steel after Various Surface Treatments. Metals 2023, 13, 1739. Hsiao, V.K.S.; Lin, Y.-C.; Wu, H.-C.; Wu, T.-I. Surface Morphology and Human MG-63 Osteoblasic Cell Line Response of 316L Stainless Steel after Various Surface Treatments. Metals 2023, 13, 1739.

Abstract

In this study, we investigated the effects of three surface treatment techniques on 316L stainless steel, low-temperature immersion corrosion, shot peening followed by immersion corrosion, and electrochemical corrosion. Surface alterations were analyzed using scanning electron microscopy, Raman spectroscopy, and α-step thin-film thickness profiling. Human osteoblast adhesion was evaluated using Liu’s stain and metallographic optical microscopy. The results revealed weak cell adhesion after low-temperature immersion and shot peening followed by immersion corrosion. By contrast, electrochemical corrosion, particularly with a high current density and low corrosive solution concentration, resulted in a uniformly corroded surface, thus promoting dense cell adhesion. All three techniques resulted in porous oxide layers. Overall, our experimental findings highlighted shot peening (1 kg/cm2 for 20 s) and electrochemical corrosion (0.5 M HCl) as effective processes. Electrochemical corrosion produced uniformly dense corrosion pits, whereas shot peening produced semicircular grooves with small corrosion pits, both favorable for cell adhesion. Electrochemical corrosion also yielded excellent cell adhesion, emphasizing its suitability for biomedical activity. We developed a process for creating a porous surface layer and thereby enhancing the cell adhesion to 316L stainless steel for potential biomedical implant applications.

Keywords

316L stainless steel; immersion corrosion; shot peening; electrochemical corrosion; cell adhesion

Subject

Chemistry and Materials Science, Materials Science and Technology

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