Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Treatment of Naturally Occurring Tendon Disease with Allogeneic Multipotent Mesenchymal Stromal Cells: A Randomized, Controlled, Triple-Blinded Pilot Study in Horses

Janina Burk
* ORCID logo ,
Liza Wittenberg-Voges
,
Susanna Schubert
,
Carolin Horstmeier
,
Walter Brehm
,
Florian Geburek
* ORCID logo
Version 1 : Received: 24 September 2023 / Approved: 27 September 2023 / Online: 27 September 2023 (10:43:19 CEST)

A peer-reviewed article of this Preprint also exists.

Burk, J.; Wittenberg-Voges, L.; Schubert, S.; Horstmeier, C.; Brehm, W.; Geburek, F. Treatment of Naturally Occurring Tendon Disease with Allogeneic Multipotent Mesenchymal Stromal Cells: A Randomized, Controlled, Triple-Blinded Pilot Study in Horses. Cells 2023, 12, 2513. Burk, J.; Wittenberg-Voges, L.; Schubert, S.; Horstmeier, C.; Brehm, W.; Geburek, F. Treatment of Naturally Occurring Tendon Disease with Allogeneic Multipotent Mesenchymal Stromal Cells: A Randomized, Controlled, Triple-Blinded Pilot Study in Horses. Cells 2023, 12, 2513.

Abstract

Treatment of tendinopathies with multipotent mesenchymal stromal cells (MSCs) is a promising option in equine and human medicine. However, conclusive clinical evidence is lacking. The purpose of this study was to gain insight into clinical treatment efficacy and to identify suitable outcome measures for larger clinical studies. Fifteen horses with early naturally occurring tendon disease were assigned to intralesional treatment with allogeneic adipose-derived MSCs suspended in serum or with serum alone by block randomization (dosage adapted to lesion size). Clinicians and horse owners remained blinded to the treatment during 12 months (7 horses per group) and 18 months (7 MSC- and 5 control-group horses) of follow-up including clinical examinations and diagnostic imaging. Clinical inflammation-, lameness- and ultrasonography scores improved more over time in the MSC-group. The lameness score difference significantly improved in the MSC- compared to the control-group after 6 months. In the MSC-group, 5 out of 7 horses were free of re-injuries and back to training until 12 and 18 months. In the control-group, 3 out of 7 horses were free of re-injuries until 12 months. These results suggest that MSCs are effective for treatment of early phase tendon disease and provide a basis for a larger controlled study.

Keywords

Tendinopathy; tendon lesion; mesenchymal stem cells; mesenchymal progenitor cells; MSC; adipose; horse serum; clinical study; re-injury; recurrence rate

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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