Version 1
: Received: 21 September 2023 / Approved: 21 September 2023 / Online: 22 September 2023 (11:21:51 CEST)
How to cite:
Imai, T.; Sudiartha, G.A.W.; Teeka, J.; Chairattanamanokorn, P.; Reungsang, A.; Hung, Y. Thermotolerance and Hydrogen Production Potential of Salt-tolerant Microflora from Salt Fields. Preprints2023, 2023091548. https://doi.org/10.20944/preprints202309.1548.v1
Imai, T.; Sudiartha, G.A.W.; Teeka, J.; Chairattanamanokorn, P.; Reungsang, A.; Hung, Y. Thermotolerance and Hydrogen Production Potential of Salt-tolerant Microflora from Salt Fields. Preprints 2023, 2023091548. https://doi.org/10.20944/preprints202309.1548.v1
Imai, T.; Sudiartha, G.A.W.; Teeka, J.; Chairattanamanokorn, P.; Reungsang, A.; Hung, Y. Thermotolerance and Hydrogen Production Potential of Salt-tolerant Microflora from Salt Fields. Preprints2023, 2023091548. https://doi.org/10.20944/preprints202309.1548.v1
APA Style
Imai, T., Sudiartha, G.A.W., Teeka, J., Chairattanamanokorn, P., Reungsang, A., & Hung, Y. (2023). Thermotolerance and Hydrogen Production Potential of Salt-tolerant Microflora from Salt Fields. Preprints. https://doi.org/10.20944/preprints202309.1548.v1
Chicago/Turabian Style
Imai, T., Alissara Reungsang and Yung-Tse Hung. 2023 "Thermotolerance and Hydrogen Production Potential of Salt-tolerant Microflora from Salt Fields" Preprints. https://doi.org/10.20944/preprints202309.1548.v1
Abstract
Upper mesophilic temperature acclimation of halophilic, hydrogen-producing bacteria from salt fields was investigated in this study, along with the changes in microbial abundance during an-aerobic digestion (AD) process. Genomic approaches such as PCR-denaturing gradient gel elec-trophoresis (DGGE) and next-generation sequencing (NGS) were performed to profile the mi-crobial communities. During AD, there was a significant abundance of Halanaerobacter lacunarum at 48°C followed by the increase in hydrogen yield, signifying potential contribution from the halophile in the hydrogen production. A decrease in the dominance of H. lacunarum and Halan-aerobium fermentans at 42°C, likely due to an increase in other bacterial species, was noted but their dominance significantly increased at temperatures of 45°C and 48°C. This investigation provides valuable insights in highlighting the potential of Halanaerobium sp. and the other halo-philic bacteria to adapt under upper mesophilic temperature conditions and synthesizing hy-drogen. The findings in the present study also underscore the importance of optimizing temper-ature and pH conditions to maximize hydrogen yield during high-salt anaerobic digestion.
Keywords
halophilic bacteria; hydrogen production; thermotolerance; upper mesophilic temperature acclimation; PCR-DGGE; next-generation sequencing; salt fields
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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