Preprint Hypothesis Version 1 Preserved in Portico This version is not peer-reviewed

Concentrating Microbes from Human Urine Samples Using Paper-Based Adsorbents for Point-of-Care Molecular Assays

Isha Uttam
,
Sujesh Sudarsan
ORCID logo ,
Rohitraj Ray
,
Raja Chinnappan
* ORCID logo ,
Ahmed Yaqinuddin
ORCID logo ,
Khaled Al-Kattan
ORCID logo ,
Naresh Kumar Mani
*
Version 1 : Received: 21 September 2023 / Approved: 22 September 2023 / Online: 22 September 2023 (08:46:17 CEST)

A peer-reviewed article of this Preprint also exists.

Uttam, I.; Sudarsan, S.; Ray, R.; Chinnappan, R.; Yaqinuddin, A.; Al-Kattan, K.; Mani, N.K. A Hypothetical Approach to Concentrate Microorganisms from Human Urine Samples Using Paper-Based Adsorbents for Point-of-Care Molecular Assays. Life 2024, 14, 38. Uttam, I.; Sudarsan, S.; Ray, R.; Chinnappan, R.; Yaqinuddin, A.; Al-Kattan, K.; Mani, N.K. A Hypothetical Approach to Concentrate Microorganisms from Human Urine Samples Using Paper-Based Adsorbents for Point-of-Care Molecular Assays. Life 2024, 14, 38.

Abstract

This hypothesis demonstrates that the efficiency of Loop-Mediated Isothermal Amplification (LAMP) for nucleic acid detection can be positively influenced by preconcentration of microbial cells onto hydrophobic paper surfaces. The mechanism of this model is based on the high attraction of microbes towards hydrophobic surfaces. Extensive studies have demonstrated that hydrophobic surfaces exhibit enhanced bacterial and fungal adhesion. The preconcentration approach by exploiting this inherent affinity of hydrophobic paper substrates enables the adherence of a greater number of target cells, resulting in a higher concentration of target templates for amplification directly from urine samples. In contrast to conventional methods, which often involve complex procedures, this approach offers a simpler, cost-effective, and user-friendly alternative. Moreover, the integration of cell adhesion, LAMP amplification, and signal readout within paper origami-based devices can provide a portable, robust, and highly efficient platform for rapid nucleic acid detection. This innovative hypothesis holds significant potential for point-of-care (POC) diagnostics and field surveillance applications. Further research and development in this field will advance the implementation of this technology, contributing to improved healthcare systems and public health outcomes.

Keywords

preconcentration; loop-mediated isothermal amplification (LAMP); Urine; microbial adhesion; hydrophobic; paper-based analytical devices (μPADs); cell surface hydrophobicity (CSH); UTI

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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