Yum, T.; Kim, E.-Y.; Kim, Y.; Choi, S.; Paeng, K.-J. The Development of an Extraction Method for Simultaneously Analyzing Fatty Acids in Macroalgae Using SPE with Derivatization for LC–MS/MS. Molecules2024, 29, 430.
Yum, T.; Kim, E.-Y.; Kim, Y.; Choi, S.; Paeng, K.-J. The Development of an Extraction Method for Simultaneously Analyzing Fatty Acids in Macroalgae Using SPE with Derivatization for LC–MS/MS. Molecules 2024, 29, 430.
Yum, T.; Kim, E.-Y.; Kim, Y.; Choi, S.; Paeng, K.-J. The Development of an Extraction Method for Simultaneously Analyzing Fatty Acids in Macroalgae Using SPE with Derivatization for LC–MS/MS. Molecules2024, 29, 430.
Yum, T.; Kim, E.-Y.; Kim, Y.; Choi, S.; Paeng, K.-J. The Development of an Extraction Method for Simultaneously Analyzing Fatty Acids in Macroalgae Using SPE with Derivatization for LC–MS/MS. Molecules 2024, 29, 430.
Abstract
The extraction method for simultaneously analyzing fatty acids (Myristic acid, cis-Palmitvaccenic acid, Palmitic acid, Palmitic-9,9-d2 acid, Oleic acid, Stearic acid and Stearic-2,2-d2 acid) related to biodiesel fuel in large algae was developed. The introduction of trimethylaminoethyl (TMAE) derivatives into the hydroxyl group (-OH) functional group of the analyte was able to improve ionization and obtain high sensitivity. Liquid Chromatography (LC) was used to investigate the optimal mobile phase composition and flow rate for the separation of five derived fatty acids. In this study, a solid phase extraction (SPE) column was introduced for efficient extraction of fatty acids, and the optimal conditions for SPE were set by comparing the recovery rate according to the solvent for each step (loading, washing, and elution) of SPE from macroalgae.
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
