New ShK-Like Peptide from the Jellyfish Nemopilema nomurai Has Human Potassium Voltage-Gated Channel-Blocking Activity

Ye-Ji Kim; Yejin Jo; Seung Eun Lee; Jungeun Kim; Jae-Pil Choi; Nayoung Lee; Hyokyoung Won; Dong Ho Woo; Seungshic Yum

doi:10.20944/preprints202309.1253.v1

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preprints.org > biology and life sciences > neuroscience and neurology > doi: 10.20944/preprints202309.1253.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

New ShK-Like Peptide from the Jellyfish Nemopilema nomurai Has Human Potassium Voltage-Gated Channel-Blocking Activity

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Version 1 : Received: 18 September 2023 / Approved: 19 September 2023 / Online: 19 September 2023 (08:18:20 CEST)

How to cite: Kim, Y.-J.; Jo, Y.; Lee, S. E.; Kim, J.; Choi, J.-P.; Lee, N.; Won, H.; Woo, D. H.; Yum, S. New ShK-Like Peptide from the Jellyfish Nemopilema nomurai Has Human Potassium Voltage-Gated Channel-Blocking Activity. Preprints 2023, 2023091253. https://doi.org/10.20944/preprints202309.1253.v1 Kim, Y.-J.; Jo, Y.; Lee, S. E.; Kim, J.; Choi, J.-P.; Lee, N.; Won, H.; Woo, D. H.; Yum, S. New ShK-Like Peptide from the Jellyfish Nemopilema nomurai Has Human Potassium Voltage-Gated Channel-Blocking Activity. Preprints 2023, 2023091253. https://doi.org/10.20944/preprints202309.1253.v1

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MDPI and ACS Style

Kim, Y.-J.; Jo, Y.; Lee, S. E.; Kim, J.; Choi, J.-P.; Lee, N.; Won, H.; Woo, D. H.; Yum, S. New ShK-Like Peptide from the Jellyfish Nemopilema nomurai Has Human Potassium Voltage-Gated Channel-Blocking Activity. Preprints 2023, 2023091253. https://doi.org/10.20944/preprints202309.1253.v1

APA Style

Kim, Y. J., Jo, Y., Lee, S. E., Kim, J., Choi, J. P., Lee, N., Won, H., Woo, D. H., & Yum, S. (2023). New ShK-Like Peptide from the Jellyfish <em>Nemopilema nomurai</em> Has Human Potassium Voltage-Gated Channel-Blocking Activity. Preprints. https://doi.org/10.20944/preprints202309.1253.v1

Chicago/Turabian Style

Kim, Y., Dong Ho Woo and Seungshic Yum. 2023 "New ShK-Like Peptide from the Jellyfish <em>Nemopilema nomurai</em> Has Human Potassium Voltage-Gated Channel-Blocking Activity" Preprints. https://doi.org/10.20944/preprints202309.1253.v1

Abstract

We have identified a new human voltage-gated potassium channel (hKv1.3) blocker, NnK-1, in the jellyfish Nemopilema nomurai, based on its genomic information. The gene sequence encoding NnK-1 contains 5,408 base pairs, with five introns and six exons. The coding sequence of the NnK-1 precursor is 894 nucleotides long and encodes 297 amino acids, containing five presumptive ShK-like peptides. An electrophysiological assay demonstrated that the chemically synthesized fifth peptide, NnK-1, is an effective hKv1.3 blocker. A multiple sequence alignment with cnidarian Shk-like peptides, which have Kv1.3-blocking activity, revealed that four residues (3Asp, 25Lys, 33Lys, and 34Thr) of NnK-1, together with six cysteine residues, are conserved. Therefore, we hypothesize that these four residues are crucial for the binding of the toxins to voltage-gated potassium channels.

Keywords

toxin; venom; jellyfish; Cnidaria; genomic information; electrophysiology

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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