Version 1
: Received: 18 September 2023 / Approved: 18 September 2023 / Online: 19 September 2023 (15:34:32 CEST)
How to cite:
McManus, W. R.; Schorey, J. S. Comparison of Ultrastructure, ECM, and Drug Susceptibility in M. avium Biofilms. Preprints2023, 2023091215. https://doi.org/10.20944/preprints202309.1215.v1
McManus, W. R.; Schorey, J. S. Comparison of Ultrastructure, ECM, and Drug Susceptibility in M. avium Biofilms. Preprints 2023, 2023091215. https://doi.org/10.20944/preprints202309.1215.v1
McManus, W. R.; Schorey, J. S. Comparison of Ultrastructure, ECM, and Drug Susceptibility in M. avium Biofilms. Preprints2023, 2023091215. https://doi.org/10.20944/preprints202309.1215.v1
APA Style
McManus, W. R., & Schorey, J. S. (2023). Comparison of Ultrastructure, ECM, and Drug Susceptibility in <i>M. avium</i> Biofilms. Preprints. https://doi.org/10.20944/preprints202309.1215.v1
Chicago/Turabian Style
McManus, W. R. and Jeffrey S. Schorey. 2023 "Comparison of Ultrastructure, ECM, and Drug Susceptibility in <i>M. avium</i> Biofilms" Preprints. https://doi.org/10.20944/preprints202309.1215.v1
Abstract
Pulmonary infections with Mycobacterium avium occur in susceptible individuals following exposure to the bacterium in the environment, where it often persists in biofilms. Many methods have been used to generate biofilms of M. avium, and it is unknown whether different approaches generate similar structures and cell phenotypes. To make a parallel comparison of in vitro biofilm ultrastructure, extracellular matrix (ECM) composition, and drug susceptibility of biofilm resident bacteria, we used two published methods to generate M. avium biofilms: four week incubation in M63 medium or 24 hour exposure to dithiothreitol (DTT). Scanning electron microscopy revealed differences in biofilm ultrastructure between the two methods, including variation in appearance of ECM materials and morphology of resident cells, while light microscopy and staining with calcofluor white indicated that both biofilms contained polysaccharides characteristic of cellulose. Measuring susceptibility of biofilms to degradation by enzymes suggested differences in structurally important ECM molecules, with DTT biofilms having important protein and, to a lesser extent, cellulose components, and M63 biofilms having moderate protein, cellulose, and DNA components. Both biofilms conferred resistance to the bactericidal effects of amikacin and clarithromycin, with resident cells being killed at >10-fold lower rates than planktonic cells at almost all concentrations. Though these comparisons indicate differences in biofilm responses by M. avium under differing conditions, they also suggest common features of biofilm formation including cellulose production and antimicrobial resistance.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
