Version 1
: Received: 14 September 2023 / Approved: 15 September 2023 / Online: 15 September 2023 (16:02:45 CEST)
How to cite:
Kenchappa, D.; Korolkova, O.Y.; Odiase, P.; Izban, M.G.; Sakwe, A.; Ochieng, J. Fetuin-A Modulates Tumor Growth and Invasion in a Basal-Like Triple Negative Breast Cancer Cell Line, MDA-MB-468. Preprints2023, 2023091088. https://doi.org/10.20944/preprints202309.1088.v1
Kenchappa, D.; Korolkova, O.Y.; Odiase, P.; Izban, M.G.; Sakwe, A.; Ochieng, J. Fetuin-A Modulates Tumor Growth and Invasion in a Basal-Like Triple Negative Breast Cancer Cell Line, MDA-MB-468. Preprints 2023, 2023091088. https://doi.org/10.20944/preprints202309.1088.v1
Kenchappa, D.; Korolkova, O.Y.; Odiase, P.; Izban, M.G.; Sakwe, A.; Ochieng, J. Fetuin-A Modulates Tumor Growth and Invasion in a Basal-Like Triple Negative Breast Cancer Cell Line, MDA-MB-468. Preprints2023, 2023091088. https://doi.org/10.20944/preprints202309.1088.v1
APA Style
Kenchappa, D., Korolkova, O.Y., Odiase, P., Izban, M.G., Sakwe, A., & Ochieng, J. (2023). Fetuin-A Modulates Tumor Growth and Invasion in a Basal-Like Triple Negative Breast Cancer Cell Line, MDA-MB-468. Preprints. https://doi.org/10.20944/preprints202309.1088.v1
Chicago/Turabian Style
Kenchappa, D., Amos Sakwe and Josiah Ochieng. 2023 "Fetuin-A Modulates Tumor Growth and Invasion in a Basal-Like Triple Negative Breast Cancer Cell Line, MDA-MB-468" Preprints. https://doi.org/10.20944/preprints202309.1088.v1
Abstract
The present studies were undertaken to address the role of fetuin-A in the growth and invasion potential in a triple negative breast cancer (TNBC) cell line, MDA-MB-468. Basal like TNBC that express high levels of ectopic fetuin-A have poorer prognosis for the patients compared to those that express low levels of the protein. We overexpressed fetuin-A in MDA-MB-468 and then determined the invasive potential of fetuin-A overexpressing cells vs controls transfected with empty vector. We also determined the adhesion and growth potential of the cells in the presence of only fetuin-A in serum free medium and also in complete medium. Our data suggest that fetuin-A overexpression significantly enhances the invasive potential of the cells and also the expression of Toll like receptor 4 (TLR4) on these cells. More importantly, the cells rely on fetuin-A-TLR4 signaling network for growth and invasion because the specific TLR4 inhibitor CLI-095 (resatorvid) abrogates fetuin-A mediated growth and invasion. Taken together, the data suggest that fetuin-A-TLR4 signaling network plays a significant role in the growth and invasion potential of TNBC.
Keywords
Fetuin-A; Triple Negative; Breast Cancer; TLR4; Invasion; Basal-like
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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