Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report

Inês Leal Reis; Bruna Lopes; Patrícia Sousa; Ana Catarina Sousa; Mariana Branquinho; Ana Rita Caseiro; Alexandra Rêma; Inês Briote; Carla Mendonça; Jorge Miguel Santos; Luís Atayde; Rui Alvites; Ana Colette Maurício

doi:10.20944/preprints202309.1058.v1

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preprints.org > biology and life sciences > animal science, veterinary science and zoology > doi: 10.20944/preprints202309.1058.v1

Preprint Case Report Version 1 Preserved in Portico This version is not peer-reviewed

Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report

Version 1 : Received: 13 September 2023 / Approved: 14 September 2023 / Online: 15 September 2023 (12:44:31 CEST)

How to cite: Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.; Caseiro, A.R.; Rêma, A.; Briote, I.; Mendonça, C.; Santos, J.M.; Atayde, L.; Alvites, R.; Maurício, A.C. Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report. Preprints 2023, 2023091058. https://doi.org/10.20944/preprints202309.1058.v1 Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.; Caseiro, A.R.; Rêma, A.; Briote, I.; Mendonça, C.; Santos, J.M.; Atayde, L.; Alvites, R.; Maurício, A.C. Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report. Preprints 2023, 2023091058. https://doi.org/10.20944/preprints202309.1058.v1

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MDPI and ACS Style

Leal Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.C.; Branquinho, M.; Caseiro, A.R.; Rêma, A.; Briote, I.; Mendonça, C.; Santos, J.M.; Atayde, L.; Alvites, R.; Maurício, A.C. Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report. Preprints 2023, 2023091058. https://doi.org/10.20944/preprints202309.1058.v1

APA Style

Leal Reis, I., Lopes, B., Sousa, P., Sousa, A.C., Branquinho, M., Caseiro, A.R., Rêma, A., Briote, I., Mendonça, C., Santos, J.M., Atayde, L., Alvites, R., & Maurício, A.C. (2023). Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report. Preprints. https://doi.org/10.20944/preprints202309.1058.v1

Chicago/Turabian Style

Leal Reis, I., Rui Alvites and Ana Colette Maurício. 2023 "Treatment of Equine Tarsus Long Medial Collateral Desmitis with Allogenic Synovial Membrane Mesenchymal Stem Cells Enhanced by Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium: Case Report" Preprints. https://doi.org/10.20944/preprints202309.1058.v1

Abstract

Horses are high performance athletes prone to sportive injuries such as tendonitis and desmitis. Fibrous tissue formation with loss of mechanical properties occurs in tendon repair, becoming a therapeutic challenge to overcome. This impels regenerative medicine to develop innovative therapies that enhance tissue regeneration retrieving original tissue properties. Mesenchymal stem cells (MSCs) have been successfully used to develop therapeutic products. They secrete a variety of bioactive molecules that play a pivotal role in tissue regeneration. These factors are released in culture media producing conditioned media (CM). The aforementioned assumptions impelled us to formulate equine synovial membrane stem cells (eSM-MSCs) – the cellular pool that naturally regenerates joint tissue, combined with medium enriched in immunomodulatory factors produced by umbilical cord stroma-derived MSCs (eUC-MSCs), that naturally contribute to suppress the immune rejection in the maternal-fetal frontier. A clinical case of an equine acute desmitis, treated with the abovementioned formulation is presented. Ligament regeneration occurred in a reduced time frame, reducing stoppage time, allowing return to unrestricted competition after completion of a physical rehabilitation program. This study focus was determination of the formulation therapeutic potential and the evaluation of its synergistic effect in an equine desmitis treatment, utilizing the cells themselves and its secretome.

Keywords

Allogenic; Case study; Ligament; Mesenchymal stem cells; MSC-based therapies; Secretome; Sport horses; Synovial membrane mesenchymal stem cell; Umbilical cord conditioned medium

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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