Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Development and Characterization of Human Oligodendroglioma Cellular Model as a Useful Tool to Study Iron Role in Tumor Cell: Preliminary Results and Future Prospective

Stefania Braidotti
ORCID logo ,
Debora Curci
,
Daniele Zampieri
ORCID logo ,
Cesare Covino
,
Davide Zanon
ORCID logo ,
Natalia Maximova
* ,
Roberto Sala
ORCID logo
Version 1 : Received: 14 September 2023 / Approved: 15 September 2023 / Online: 15 September 2023 (09:40:22 CEST)

A peer-reviewed article of this Preprint also exists.

Braidotti, S.; Curci, D.; Zampieri, D.; Covino, C.; Zanon, D.; Maximova, N.; Sala, R. Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells. Biomedicines 2023, 11, 2940. Braidotti, S.; Curci, D.; Zampieri, D.; Covino, C.; Zanon, D.; Maximova, N.; Sala, R. Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells. Biomedicines 2023, 11, 2940.

Abstract

Oligodendroglioma (OG) is a brain tumor that contributes to < 1% of brain tumor diagnoses in the pediatric population. Unfortunately, pediatric OG remains without definitive molecular characteristics to aid in diagnosis, and little is known about the tumor microenvironment. Tumor cells' metabolism and proliferation rate are generally higher than healthy cells, so their iron demand is also significantly increased. This consideration underlines the great importance of iron for tumor development and progression. In this context, this study aims to evaluate the effect of iron in a cellular in vitro model of human oligodendroglioma brain tumor. Cell morphology, the effect of siderotic medium on cell growth, iron uptake, and the expression of iron metabolism-related genes were evaluated by optic microscopy, ICP-MS, confocal microscopy, and RealTime PCR, respectively. This study underlines the great importance of iron for tumor development and progression, but also the possibility of reducing the available iron concentration to determine an antiproliferative effect on OGs. Therefore, every attempt can be promising to defeat the OGs for which there are currently no long-term curative therapies.

Keywords

Oligodendroglioma; iron; pediatric; brain tumors

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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