Yu, X.; Zhang, Y.; Hou, L.; Qiao, X.; Zhang, Y.; Cheng, H.; Lu, H.; Chen, J.; Du, L.; Zheng, Q.; Hou, J.; Tong, G. Increases in Cellular Immune Responses Due to Positive Effect of CVC1302-Induced Lysosomal Escape in Mice. Vaccines2023, 11, 1718.
Yu, X.; Zhang, Y.; Hou, L.; Qiao, X.; Zhang, Y.; Cheng, H.; Lu, H.; Chen, J.; Du, L.; Zheng, Q.; Hou, J.; Tong, G. Increases in Cellular Immune Responses Due to Positive Effect of CVC1302-Induced Lysosomal Escape in Mice. Vaccines 2023, 11, 1718.
Yu, X.; Zhang, Y.; Hou, L.; Qiao, X.; Zhang, Y.; Cheng, H.; Lu, H.; Chen, J.; Du, L.; Zheng, Q.; Hou, J.; Tong, G. Increases in Cellular Immune Responses Due to Positive Effect of CVC1302-Induced Lysosomal Escape in Mice. Vaccines2023, 11, 1718.
Yu, X.; Zhang, Y.; Hou, L.; Qiao, X.; Zhang, Y.; Cheng, H.; Lu, H.; Chen, J.; Du, L.; Zheng, Q.; Hou, J.; Tong, G. Increases in Cellular Immune Responses Due to Positive Effect of CVC1302-Induced Lysosomal Escape in Mice. Vaccines 2023, 11, 1718.
Abstract
Here, we found higher percentage of CD8+ T cells in piglets immunized with CVC1302-adjuvanted inactivated FMDV vaccine. We wonder whether promoted cellular immunity induced by CVC1302-adjuvanted inactivated FMDV vaccine due to the promoted antigen cross-presentation efficiency of OVA by DC mainly via the cytosolic pathways, demonstrated by enhanced levels of lysosomal escape of OVA in DC, loaded with OVA and CVC1302. Higher levels of ROS and significant enhanced elevated lysosomal pH in DC facilitated the lysosomal escape of OVA. Significant enhanced CTL activities were observed in mice immunized with OVA-CVC1302. Overall, CVC1302 increased the cross-presentation of exogenous antigens and the cross-priming of CD8+ T cells by alkalizing the lysosomal pH and facilitating the lysosomal escape of antigen. These studies shed new light on the development of immunopotentiators to improve the cellular immunity induced by vaccine.
Biology and Life Sciences, Immunology and Microbiology
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