Version 1
: Received: 12 September 2023 / Approved: 13 September 2023 / Online: 14 September 2023 (10:30:19 CEST)
How to cite:
Bayazid, A.B.; Jeong, S.A.; Azam, S.; Oh, S.H.; Lim, B.O. Neuroprotective Effects of Fermented Blueberry and Black Rice Against Particulate Matter 2.5 μm-Induced Inflammation In Vitro and In Vivo. Preprints2023, 2023090958. https://doi.org/10.20944/preprints202309.0958.v1
Bayazid, A.B.; Jeong, S.A.; Azam, S.; Oh, S.H.; Lim, B.O. Neuroprotective Effects of Fermented Blueberry and Black Rice Against Particulate Matter 2.5 μm-Induced Inflammation In Vitro and In Vivo. Preprints 2023, 2023090958. https://doi.org/10.20944/preprints202309.0958.v1
Bayazid, A.B.; Jeong, S.A.; Azam, S.; Oh, S.H.; Lim, B.O. Neuroprotective Effects of Fermented Blueberry and Black Rice Against Particulate Matter 2.5 μm-Induced Inflammation In Vitro and In Vivo. Preprints2023, 2023090958. https://doi.org/10.20944/preprints202309.0958.v1
APA Style
Bayazid, A.B., Jeong, S.A., Azam, S., Oh, S.H., & Lim, B.O. (2023). Neuroprotective Effects of Fermented Blueberry and Black Rice Against Particulate Matter 2.5 μm-Induced Inflammation In Vitro and In Vivo. Preprints. https://doi.org/10.20944/preprints202309.0958.v1
Chicago/Turabian Style
Bayazid, A.B., Seung Hyeon Oh and Beong Ou Lim. 2023 "Neuroprotective Effects of Fermented Blueberry and Black Rice Against Particulate Matter 2.5 μm-Induced Inflammation In Vitro and In Vivo" Preprints. https://doi.org/10.20944/preprints202309.0958.v1
Abstract
The escalating prevalence of particulate matter (PM) has raised serious concerns regarding its detrimental effects on human health. This study aimed to investigate the potential of fermented blueberry and black rice (FBBR) in mitigating the effects of PM2.5, both in SH-SY5Y cells and mice exposed to PM2.5. Various assays, including MTT, NO, western blot, ELISA, and behavioral studies (MWM and Y-maze) were conducted. Our results demonstrated that PM2.5 induced significant cytotoxicity and elevated nitric oxide (NO) production at a concentration of 100μg/mL of PM2.5 in SH-SY5Y cells. Additionally, administration of FBBR effectively attenuated PM2.5-induced cytotoxicity and suppressed NO production in SH-SY5Y cells. In an intranasal instilled mice model, exposure to 10 mg/kg body weight of PM2.5 resulted in cognitive impairments. However, FBBR treatment ameliorated these impairments in both the Y-maze and MWM tests in PM2.5-exposed mice. Furthermore, FBBR administration increased the expression of brain-derived neurotrophic factor (BDNF) and reduced inflammatory markers in the brains of PM2.5-exposed SH-SY5Y cells. These findings underscore the detrimental effects of PM2.5 on the nervous system and highlight the potential of FBBR as a nutraceutical agent for mitigating these effects. Overall, this study emphasizes the urgency of addressing the harmful impact of PM2.5 on the nervous system and suggests the promising role of FBBR as a protective intervention against the adverse effects associated with PM2.5 exposure.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
