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Clinical Applications of Combined İmmunotherapy Approaches in Gastrointestinal Cancer: A Case-Based Review

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Submitted:

12 September 2023

Posted:

13 September 2023

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Abstract
Malignant neoplasms arising from the gastrointestinal (GI) tract are among the most common cancer types with a high mortality rate. Despite advances in treatment in a small subgroup harboring targetable mutations, the outcome remains poor, accounting for one in three cancer-related deaths observed globally. As a promising therapeutic option in various tumor types, immunotherapy with immune checkpoint inhibitors has also been evaluated in GI cancer, albeit with limited efficacy except for a small subgroup expressing microsatellite instability. In the quest for more effective treatment options, energetic efforts have been placed to evaluate the role of several immunotherapy approaches comprising of cancer vaccines, adoptive cell therapies and immune checkpoint inhibitors. In this review, we report our experience with personalized dendritic cell cancer vaccine and cytokine-induced killer cell therapy in three patients with GI cancers and summarize current clinical data on combined immunotherapy strategies.
Keywords: 
gastrointestinal cancer; immunotherapy; cancer vaccines; dendritic cell vaccine; adoptive cell treatment; cytokine-induced killer cells; immune checkpoint inhibitors; angiogenesis inhibition
Subject: 
Medicine and Pharmacology  -   Oncology and Oncogenics

Introductıon

Despite improvements in screening and treatment, gastrointestinal (GI) tract cancers are a major contributor to the global cancer burden. According to the Globocan statistics, with 3,574,000 new cases, the incidence of esophageal, gastric and colorectal cancers (CRC) represents 19.7% of all cancers diagnosed in 2020, leading to 22.5% of all cancer-related deaths (1). In the US, with 213000 new cases and 85000 deaths projected for 2023, GI cancers rank 3rd in incidence and mortality among all sites diagnosed with cancer. Although 5-year survival rates have been improved for all stages combined in both colorectal (CRC) and gastric cancers, with mortality rates steadily decreasing over the last decade; but need to be improved for both tumor types as GI-related cancer deaths comprise about 13.7% of all cancer deaths and an annual rise in mortality by 1% is noted among young adults with CRC (2,3).
With the advent of modern immunotherapy, a new era of cancer therapy has been initialized. Markedly improved survival rates have been achieved with immune checkpoint inhibitors for several cancer types, which had otherwise been considered the most lethal of all cancer types. Programmed-death ligand-1 (PD-1) inhibitors, namely nivolumab and pembrolizumab have been approved for the upfront treatment of advanced esophagogastric cancers combined with chemotherapy based on the significantly improved survival rates with hazard ratios (HR) ranging between 0.71 and 0.74 (4,5). Furthermore, monotherapy with immune checkpoint inhibitors has yielded favorable response rates and prolonged survival rates in microsatellite instable /mismatch repair deficient cancers as compared to standard chemotherapy, earning a rightful designation of “the game-changer treatment” even in heavily pretreated groups. (6-10). Nevertheless, accumulating data have shown that the majority of patients still succumb to their disease, indicating a requirement for novel approaches to overcome resistance.
Immunosuppressive microenvironment (TME), inadequate effector immune response and function of cancer-specific cytolytic CD8 (+) T cells, inefficient antigen presentation as well as epigenetic modifications that control immune regulation are major mechanisms that are implicated in acquired resistance to immune-mediated cell killing (11). Addressing some of these tumor-escape pathways, cancer vaccines and adoptive transfer of T cells combined with chemotherapy, anti-angiogenic agents and radiotherapy have been established as complementary methods to induce an effective anti-cancer immune response (12).
In this review, we will discuss the role of immunotherapy in GI tract cancers based on our experience with combined strategies and place this in context with emerging evidence from clinical trials. We hope that this overview will provide insight into comprehensive immunotherapy approaches not only for GI cancers, but for all cancer types where immune-associated cell killing is warranted.

II. Cancer immunology and immunotherapy

The immune system is comprised of myeloid cells such as lymphocytes and macrophages that are differentiated to generate an immune response when encountered with foreign appearing structures in the host, including cancerous cells. When tumor cells are identified by the innate immune system, cellular fragments or foreign antigens from these cells are internalized by the antigen-presenting cells (APC), such as dendritic cells or macrophages, which are the main components of the immune system that are responsible for antigen cross-presentation to prime specific B and T cell responses (13).
Dendritic cells are the most potent antigen-presenting cells in the human immune system. Dendritic cells, which can activate naive T cells against various host attacks, including cancer, belong to the mononuclear phagocyte system and are professional antigen-presenting cells (14). First discovered by Paul Langerhans in 1868, dendritic cells were named after cells that exhibited long cytoplasmic processes in the mouse spleen in 1973. Various subsets of dendritic cells originate from a unique hematopoietic lineage in the bone marrow (15,16). In addition to the activation of innate immunity, it plays an important role in the stimulation of cytotoxic T lymphocyte-mediated acquired immunity. Immature forms of dendritic cells circulate widely in the blood and peripheral tissues, where they contain pathogen-infected or tumor cell-derived antigens. After antigen uptake, immature dendritic cells undergo phenotypic and functional maturation. They migrate to secondary lymphoid organs and tissues, such as lymph nodes, presenting processed antigens and activating cytotoxic T lymphocytes (15-17). Thus, mature dendritic cells trigger an antigen-specific immune response to eliminate the antigen-expressing target cells. In addition, mature dendritic cells can increase the cytotoxic activity of NK cells, which, like immature dendritic cells, act as innate immune effector cells to destroy pathogen-infected or tumor cells (18).
Like other leukocytes, dendritic cells develop from bone marrow-derived hematopoietic stem cells (CD34 + active) and differentiate from common myeloid progenitors. However, a small fraction of dendritic cells may arise from common lymphoid progenitor cells (19). In the downregulation of common myeloid progenitors, macrophage/dendritic cell progenitor cells serve to provide the host with a constant source of monocytes, macrophages, and classical dendritic cells. Specifically for dendritic cell development, macrophage/dendritic cell progenitors give rise to common dendritic cell progenitor cells that expand into progenitor dendritic cells. These cells migrate to lymphoid and non-lymphoid organs to mature further into functional dendritic cell subtypes based on intrinsic and/or extrinsic factors (20, 21). When dendritic cells are found in peripheral tissues, they represent the surrounding environment via receptor-mediated phagocytosis or macro-pinocytosis (21, 22). Following antigen uptake, dendritic cells respond to the chemokines CCL19 secreted by mature dendritic cells or CCL21 as lymphatic vessel-derived endothelial cells and migrate to draining lymph nodes, via upregulation of CCR7 (23, 24). During antigenic transfer, dendritic cells assume a phenotype of mature antigen-presenting cells marked by increased expression of surface molecules, such as major tissue compatibility complex class II (MHC-II) and CD80/CD86. The dendritic cells then bind and present the transduced antigen to the T cells in the lymph node paracortex via the MHC. This provides the necessary co-stimulation and cytokine support for T-cell activation and proliferation (18, 23).
Once antigens are processed and loaded onto MHC I and II molecules, APC’s interact with the microenvironment to present the neoantigen to cytotoxic T cells and B cells, respectively, which are subsequently activated to develop an antigen-specific immune response [13,25]. Nevertheless, during tumor progression, the anti-cancer immunologic activity may be restricted by the upregulation of various checkpoints and immune-suppressive elements of the host immune system, which is inherently programmed to balance any excessive immune activity against the host. It has been shown that recently identified inhibitory immune checkpoints such as LAG-3, TIGIT and IDO control immune activity by suppressing tumor-specific T and B lymphocytes, resulting in a shift towards generation of an immune-suppressive stroma comprised of regulatory T cells (Treg), immature dendritic cells, M2 macrophages and myeloid-derived suppressor cells (MDSC) [26]. It has been shown that cancer cells interact with the host immune system to overcome anti-tumor immunity by switching the tumor microenvironment to an immuno-suppressive or immune-cold phenotype mediated by several cytokines and molecules [26-28].
Immunotherapy refers to various treatment strategies that aim to restore a dysregulated immune function or to modulate the host immune system to generate an adequate immune-mediated anti-tumor response. The current immunotherapy approaches address several mechanisms that hamper generation of a sufficient immune response for immune-mediated cell killing. Immune checkpoint inhibitors are monoclonal antibodies that aim to release the brakes on immune cells by blockade of PD-1, PD-L1 or CTLA receptors. As mentioned above, nivolumab and pembrolizumab are PD-1 inhibitors that have been approved for the treatment of gastric and CRC for various indications. Other investigational approaches for immunotherapy target the immune system to generate a cancer-specific response by cancer vaccines or to administer ex-vivo activated autologous or allogeneic immune cells that target cancer cells, such as tumor-associated lymphocytes (TIL), CAR-T cells or engineered NK cells, otherwise referred to as adoptive cell therapy [29-34]. Detailed information on these strategies will be provided in context with current evidence in GI immunotherapy in the corresponding sections below.

III. Immunotherapy in GI cancers: Cellular treatment systems

a. Cancer vaccines: rationale, limitations and potential combinations

The history of immunization for cancer therapy dates back to 1890, when Sir William Coley treated cancer patients with streptococcal bacterial cultures and reported tumor regressions (35). Since then, enormous efforts have been placed to design an efficient system, which would stimulate the host immune system to elicit an adequate and specific immune response against cancer cells. Biologic systems that aim to prime T and B lymphocytes by introducing tumor neoantigens via distinct delivery systems are called cancer vaccines. The neoantigens are provided as inactivated whole tumor cells or components of cells that convey specific characteristics, such as nucleic acids or peptides, which lead to the recognition of the cancer cells by effector lymphocytes of the host immune system. These antigens are delivered through several systems including dendritic cells, viral particles, exosomes, or synthetic systems such as lipid and polymer-based nanoparticles. As an active immunotherapy strategy, the goal of treatment with cancer vaccines is not only to induce a cancer-specific response, but also to establish immunologic memory for a sustained and prolonged immune-mediated cell-killing effect (36-38). Despite the enthusiasm generated over immunotherapy in the past century, the clinical efficacy of cancer vaccines has been underwhelming except for Spileucel-T, an autologous tumor cell-based vaccine, which was approved for routine clinical use based on overall survival advantage for patients with castration-resistant prostate cancer and Talimogene Laherperepvec for malignant melanoma based on efficacy to elicit durable tumor responses in remote lesions (39-41).

Dendritic cell vaccines

Human dendritic cell subsets loaded with tumor-associated antigens, referred to as dendritic cell vaccines have emerged as a promising immunotherapeutic tool for the treatment of many cancer types, including colorectal cancer, breast cancer, and hepatocellular carcinoma (42-44). The mainstay of designing dendritic cell vaccines is the development of an effective and durable cytotoxic T-lymphocyte immune response by inducing both humoral and cellular immune responses that, together with the immune capacities of dendritic cells, induce the clonal expansion of T cells (45). Once activated, dendritic cells have the capability to migrate to the nearest lymph nodes, which is required to initiate immunologic mechanisms leading to the generation of humoral and cellular immune responses against tumors (46, 47). Generally, vaccine production involves isolation of immature dendritic cells or precursors from the blood, followed by ex vivo maturation and activation through co-culturing with cytokine cocktails. The generated mature dendritic cells are then pulsed with autologous tumor cells or neoantigens to form dendritic vaccines which are administered back to the patient (48). Encouraging preclinical data on the anti-tumor efficacy of dendritic cells packed with tumor lysates led to intensive clinical evaluation of dendritic vaccines by the end of the last century (49). Subsequent clinical trials utilizing antigen-specific dendritic cells showed partial responses coupled with anti-tumor immune responses in several tumor types including B-cell lymphoma, melanoma and hepatocellular carcinoma (50-53).

Cancer vaccines and combined strategies to improve immunogenic activity in GI cancers

Nevertheless, cancer vaccines against pre-identified tumor neoantigens in CRC have shown limited efficacy, as the “cold” tumor microenvironment (TME) has precluded the generation of an adequate specific cytotoxic T cell response. Therefore, energetic efforts are being placed to overcome the immunosuppressive TME and turn “cold” tumors into “hot” tumors, where an effective immune-mediated response can be triggered through activation of CD8 (+) T cells, Th1 B cells as well as inhibition of Tregs, tumor-associated macrophages (TAM’s) and proinflammatory signals such as TNF-alpha. Among many strategies, activating cytokine adjuvants used along with vaccines have shown enhanced tumor-specific immune responses. In a murine model with CRC, GM-CSF and IL-2 combination with a vaccine targeting WNT as a neoantigen, has yielded an augmented T cell response which translated into an effective tumor cell killing (54). Despite discrepant effects of interleukins in the ability to induce both pro-inflammatory and anti-inflammatory responses in the TME, pretreatment with IL-2 has also been shown to improve the efficacy of chemotherapy in a group of metastatic CRC patients by increasing lymphocyte proliferation (55). Interferon-alpha is another cytokine that plays an important role in generating an immune stimulatory response through upregulating MHC-I molecules on the surface of dendritic cells and in turn improving neoantigen presentation. Furthermore, the anti-angiogenic effect of IFN-alpha helps overcome a significant resistance mechanism by converting a cold TME into an immune-friendly stroma (56, 57).
As mentioned above, immune checkpoint inhibitors have not shown clinical efficacy in microsatellite stable (MSS) GI tumors through mechanisms that involve increased T cell exhaustion and activation of co-inhibitory signals such as TIM3 and Lag3 (58-60). In fact, combined inhibition of PD-1 and TIM-3 pathways have resulted in enhanced cytotoxic T cell responses to autologous dendritic cell vaccination in CRC patients, addressing an unmet need in this patient population (61). Chemotherapy has also been shown to activate the immune system by modifying the TME and may have a potential role in converting “cold” into a “hot” TME with a high TIL infiltration (62). As hypothesized, chemotherapy and CEA vaccine combinations have yielded increased antigen-specific T cell responses and improved clinical responses in earlier clinical trials, providing a strong rationale for integrating cytotoxic agents in vaccination protocols (63, 64).
Angiogenesis, which plays a significant role in escape mechanisms by supplying nutrients to a growing tumor tissue, is a relevant target in carcinogenesis (65). Further evidence suggests a possible interplay with angiogenesis and the TME that interferes with immune cell adhesions and migration across the vascular-endothelial junctions, thus preventing infiltration of immune effector cells within the tumor tissue (66). The hypoxic tumor environment mediated through activation of VEGF and angiopoietin pathways provides an unfriendly stroma not only by inactivation of cytotoxic T cells, but also by promoting expansion of inhibitory components of the immune system including Tregs and TAMs (67). Earlier observations that have shown inhibition of Tregs with bevacizumab and activation of mature dendritic cell and T cell proliferation lend support to the potential role of anti-angiogenic therapy in generating an immune-friendly TME (68, 69).

Cancer vaccines for esophageal and gastric cancer: clinical evidence

Earlier clinical trials evaluating the role of dendritic vaccines pulsed with peptides including Her-2 and MAGE have shown the feasibility of this approach with a low toxicity profile and tumor-specific T cell responses which translated into minor tumor regressions (70-72). More recent trials focused on combination strategies aiming to improve immune responses as well as clinical efficacy. A peptide vaccine combining URLC10 peptide, a neoantigen frequently expressed in gastric cancer and VEGFR1, an anti-angiogenic epitope was evaluated in a cohort of refractory metastatic gastric cancer patients. Although specific cytotoxic T cell responses were detected in 65% of patients, 30% had disease stabilization as the best response and a poor outcome with a median survival of 3.6 months (73). In a phase Ib trial, 14 patients with Her-2 overexpressing metastatic gastric cancer were treated with a Her-2 peptide vaccine selected for B cell epitopes combined with a non-toxic form of diphtheria toxin, which is not only intended to prime specific memory T cell responses, but also blocks cellular proliferation through targeting heparin-binding receptors on tumor cells. Out of 11 patients eligible for response assessment, the overall response rate was reported as 54.5%, with one patient who achieved a complete response (74).
Several trials evaluated the efficacy of vaccines combined with chemotherapy in the advanced setting and as part of adjuvant chemotherapy in patients who underwent surgery for localized gastric cancer. The GC4 was a multicenter trial that enrolled advanced gastric and gastroesophageal cancer patients investigating the role of chemotherapy and a peptide vaccine from the gastrin sequence conjugated with diphtheria toxin. Previously untreated 96 patients received standard treatment with cisplatin and fluorouracil in combination with the vaccine administered intramuscularly in weeks 1, 5, 9 and 25, yielding a confirmed ORR of 30% in evaluable patients. The median TTP and OS were estimated as 5.4 and 9.2 months, respectively; with a significant improvement in immune-responders as compared to those who failed to generate circulating anti-G17 antibody titers (75). A similar approach of dendritic cell vaccination pulsed with WNT and MUC1 peptide given in combination with salvage chemotherapy was evaluated in 20 patients with gastric cancer. The treatment was feasible and a trend for prolonged survival was observed in patients in whom a higher percentage of effector T cells could be induced (76). As an adjuvant strategy, a multi-epitope vaccine combining neoantigens with angiogenic peptides was evaluated in 14 patients who received adjuvant chemotherapy for stage III resected gastric cancer. The combined approach was found feasible, but outcomes were not reported (77).
Immune checkpoint inhibitors revolutionized immunotherapy for the treatment of numerous cancer types. From a mechanistic point of view, these agents are hypothesized to boost cancer-specific cytotoxic T cell responses by releasing the brakes on the immune system. This strategy was explored in a patient with metastatic gastric cancer as part of an ongoing clinical trial investigating a dendritic cell vaccine pulsed with eight tumor-associated neoantigens. The patient received four vaccines after lymphodepletion with cyclophosphamide followed by boosters given in combination with nivolumab every two weeks starting from day 65, leading to a rapid tumor response despite progression over the first two months of single-agent vaccine therapy. The patient displayed a complete response which was ongoing after 25 months (78). Ongoing and completed clinical trials with vaccines in upper GI malignancies are listed in Table 1.

Cancer vaccines for colorectal cancer: clinical evidence

As a universal biomarker, carcinoembryonic antigen (CEA) is a frequently utilized tumor antigen in CRC vaccine trials. Early phase I trials evaluating the role of DC vaccines pulsed with CEA have demonstrated potent T cell responses in a small cohort of CRC patients with liver metastases. Correlations with outcomes could not be analyzed due to the small sample size (79). Another study investigating the role of an engineered adenovirus construct encoding a modified HLA-restricted CEA included 32 patients with CRC who had progressed following a median of three previous lines of standard chemotherapy. These patients who received three subcutaneous injections every three weeks were found to generate polyfunctional CD 8 (+) cytotoxic T cells and exhibited an encouraging 12-month survival rate of 48% (80). Similarly, a MUC-1 peptide vaccine given as three subcutaneous injections with a booster dose at week 52 was shown to generate weakly increased memory cell immune responses in a cohort of 37 patients with CR adenomas, suggesting possible efficacy as a preventive strategy (81). A recombinant vaccinia virus-based vaccine encoding multiple neoantigens including CEA, MUC-1, ICAM and LFA was evaluated in a randomized study including CRC patients who had undergone resections for liver or lung metastases. Seventy-six patients were enrolled in the trial, which aimed to investigate the role of two different vaccine constructs in a minimal residual disease setting. Translational analysis showed a high ratio of CEA-specific T cell responses. Although there was no difference between the two groups, the median RFS and OS rates reported as 25.7 and 44.1 months, respectively, were higher than contemporary unvaccinated controls (82). A recent study evaluated the role of an immunomodulatory vaccination with arginase-I (ARG-I) peptide, which targets the immunosuppressive tumor microenvironment (TME) in a small cohort with multiple tumor types including CRC. Nevertheless, despite generation of a specific T and B cell immune response, there were no objective responses attained with the new approach (83).
Further clinical trials integrated innovative immunostimulatory strategies in vaccination schedules to improve response rates. One of these, which included 53 advanced cancer patients comprising 38 patients with CRC evaluated the efficacy of a Ras-peptide vaccine. Patients received 3 doses of the vaccine every 3-5 weeks with GM-CSF or IL-2 as immunostimulatory adjuvants. The study demonstrated that almost all patients who received GM-CSF developed an immune response by the ELISPOT assay. An unplanned subgroup analysis of CRC patients revealed an encouraging OS of 14.2 months, which compared favorably to historical controls who had a median OS of 12.9 months, despite the shorter median PFS of 3.5 months in the vaccinated cohort. This interesting observation lends support to the generally accepted notion that response to immunotherapy and overall survival are not correlated (84).
Numerous clinical studies have utilized chemotherapy as an immune modulatory approach to improve efficacy of cancer vaccines. Barve et al (85) have reported two cases who had been treated with an autologous tumor vaccine given in conjunction with the FOLFOX regimen following resection of liver metastases. The vaccine consisted of surgically resected autologous tumor samples electroporated with a plasmid encoding GM-CSF and a bifunctional siRNA targeting immunosuppressant cytokines. Patients received subcutaneous two injections every two weeks followed by maintenance every four weeks until the product was exhausted along with six cycles of chemotherapy, which resulted in a disease-free survival extending beyond eight years. A similar approach was evaluated in a phase I trial that evaluated the role of a peptide vaccine containing seven tumor neoantigens combined with chemotherapy as an add-on maintenance strategy in the minimal residual disease setting. In addition to sustained tumor-specific T cell responses, a confirmed ORR of 27.5% and a clinical benefit rate of 63.6% was achieved with the combination in a cohort of 11 patients with metastatic CRC (86).
Emerging clinical evidence suggests a possible role for immune checkpoint inhibitors in priming T cell responses in an otherwise immune-suppressed TME, such as microsatellite stable CRC. A phase II clinical trial evaluated the role of GVAX, an allogeneic, whole-cell cancer vaccine given in combination with pembrolizumab and low-dose cyclophosphamide in patients who had progressed after at least two prior lines of chemotherapy for mismatch repair proficient metastatic CRC. Unfortunately, the trial was terminated due to futility after enrolment of 17 patients, in whom no objective responses were observed despite a substantial biochemical response (87). Similarly, a combination of avelumab and an autologous dendritic cell vaccine yielded no objective responses in a small phase II study comprising of a heavily pretreated cohort with metastatic CRC (88). Nevertheless, a phase I trial investigating the role of a combined viral-based multiepitope vaccine comprising of a chimpanzee adenovirus and Venezuelan equine encephalitis virus-based vectors has been shown to elicit long-term memory immune responses in 14 patients comprising of 7 MSS metastatic CRC and 8 patients with metastatic GC. In the expanded phase 9 patients (3 GC, 6 CRC) received subcutaneous low-dose anti-CTLA monoclonal antibody (ipilimumab) or PD-1 therapy with nivolumab along with vaccination. In this cohort, 1 patient with GC had a complete response and was alive after 470 days. In the whole cohort, the majority of patients had a stable response. For patients with MSS-CRC, 3 of 7 patients remained alive with an OS of 42% at 12 months, suggesting that some patients may derive durable clinical benefit despite the lack of a radiologic response by imaging (89).
Angiogenic inhibition with bevacizumab has recently been explored as a distinct approach to overcome the immune suppressive environment in a phase II randomized trial. Patients with untreated MSS metastatic CRC were randomized to FOLFOX plus bevacizumab as standard treatment or the same regimen combined with avelumab and an adenovirus-based vaccine encoding CEA. The trial was terminated early after an unplanned futility analysis conducted with eight patients, showing no difference in PFS (90).
Despite the capability to generate robust cancer-specific T cell responses, the inadequate clinical efficacy of cancer vaccines combined with several immunomodulatory strategies highlights the requirement for the identification of subgroups who benefit from these therapies, as well as innovative approaches that would boost immunogenic anti-cancer responses. Ongoing and completed clinical trials with vaccines in CRC are listed in Table 2.

b. Adoptive cell therapy: cytokine-induced killer cells as an immunotherapy approach in GI cancers

Adoptive cell therapy (ACT) is a type of personalized cellular immunotherapy, that involves isolation of immune cells from the blood and re-administration following ex-vivo expansion. Initial evidence regarding the efficacy of ACT has been generated from earlier melanoma trials, which have demonstrated response rates ranging between 49-72%, and long-term survival achieved in a fraction of patients (91). Cytokine-induced killer cells (CIK) represent a fraction of expanded lymphocytes that are comprised of CD3+CD56+ natural killer cells (NK), which are capable of MHC-unrestricted immune cell killing, as well as CD3+CD56-T cells. Although NK cells are the major effector cells, the anti-tumor efficacy of CIK cells is enhanced through the induction of cytokines that upregulate Th1 type immune response and promote migration to the tumor site following infusion. The main advantage of CIK cells is their potential efficacy against a wide array of tumors, MHC-unrestricted activity and less demanding technical requirements, which have led to the widespread use of this promising strategy as an adoptive immunotherapy tool in several tumor types (92, 93).
Numerous clinical trials have evaluated the feasibility and efficacy of CIK cell therapy in GI cancers. One of the earlier studies randomized 60 patients with metastatic CRC to standard chemotherapy versus 1-4 cycles of CIK cycles in combination with 6 cycles of FOLFOX chemotherapy. The majority of these patients had undergone surgical resection of metastatic sites. The investigators reported a significant improvement in PFS (25.8 vs. 12 months; p:0.01) and OS (41.3 vs. 30.8 months; p:0.037) in the combined treatment group (94). A phase II clinical study including 33 patients with stage IV GI cancers evaluated the role of combined CIK and CAPOX chemotherapy in the first-line setting. Two CIK infusions were administered on days 14 and 16 in the first couple of chemotherapy cycles in the first group, whereas the second group of 17 patients received chemotherapy only. Numerically longer PFS and OS rates were achieved with the combination despite failure to show a significant difference (PFS: 5.6 vs 3.8 months, p:0.06; OS: 13.9 vs 11 months in combined versus chemotherapy arms respectively). Although the CD8 (+) ratio was not different, patients in the combined arm showed an improved humoral immune response with increased NK, and CD4 (+) helper T cells (95). In a prospective phase II trial by Zhao et al (96) 122 patients were randomized to autologous CIK cells combined with chemotherapy or to chemotherapy alone. The chemotherapy arm consisted of the FOLFOX regimen given for 12 cycles every two weeks and CIK cells were administered on days 15 and 16 of each cycle. As often encountered in immunotherapy trials, with the combined treatment a significant OS (36 vs. 16 months, p<0.001) was achieved, despite a numerically longer PFS not reaching significance levels (16 vs. 10 months, p:0.07).
The impact of CIK cell-based immunotherapy was also investigated by several trials in the adjuvant setting following resection for early-stage CRC. In a retrospective analysis comprised of 96 patients who received at least one CIK infusion during adjuvant chemotherapy, a significant improvement in DFS was achieved with a HR:0.28 (p:0.034) (97). A small prospective randomized phase II study enrolling 46 patients reported improved quality of life and longer OS with the combined CIK and adjuvant FOLFOX regimen (41.9 months) as compared to those who received only chemotherapy (33.8 months) (98). A retrospective study evaluated the role of combined CIK treatment and chemotherapy in a cohort of 60 patients with a control arm comprising of 62 patients who received chemotherapy. Despite a higher ratio of stage III patients in the CIK arm, a significantly higher 5-year DFS (70.7 % vs. 48.3%, p: 0.0024) and OS rate (88.7% vs. 72.4%; p:0.008) were achieved with the combination arm as compared to controls (99).
The encouraging efficacy of adoptive immunotherapy with CIK cells in CRC, either given as a single treatment or in combination with DC was confirmed in a meta-analysis, which included data from 70 studies involving 6743 patients with all stages, of whom 66.7% had stage IV disease. Twenty-five studies included CIK as the only investigational procedure. A substantial difference in PFS (HR:0.63, p<0.00001) and OS (HR:0.57, p<0.00001) was observed with CIK cell immunotherapy as compared to non-immunotherapy treatment (100).

c. Combined immunotherapy approaches: Rationale and preclinical evidence

Favorable outcomes achieved with CIK cell therapy led to increased efforts to improve efficacy with combined approaches. There exists strong preclinical evidence on the synergistic efficacy of pro-inflammatory cytokines such as interleukins (101-104) and interferon (105) as well as cytotoxic agents (106), novel small molecule inhibitors (107-109), immune checkpoint inhibitors (110-112), monoclonal antibodies (113, 114) and dendritic cell (115-117) or viral-based vaccines (118-120) combined with adoptive cell therapy. Several stromal or intracellular mechanisms implicated in the synergistic activity have been identified. As mentioned above cytokines and cytotoxic agents improve immune-mediated cell killing through TME modulation not only by decreasing the ratio of Tregs, but also by induction of a memory effector cell response (102, 104) or direct anti-tumor effect by blockade of cytokine-receptors (105). Co-incubation with dendritic cells has yielded a higher percentage of CD3, CD4 (+) helper T and CD8 (+) T cell accumulation in cell cultures, resulting in improved anti-proliferative and cytotoxic effects not only through induction of immune-mediated cell killing, but also direct activation of CIK cells through CCR5 signaling (115, 116). Small molecule inhibitors that interfere with immunosuppressive pathways such as angiogenesis or beta-catenin-mediated CXCR3 chemokines have also been shown to enhance effector T and NK cell infiltration within the tumor stroma, thus inducing a friendly T-cell inflamed TME (107, 109). As a complementary strategy, immune checkpoint inhibitors augment the anti-tumor efficacy of CIK cells by neutralizing PD-1 and PD-L1 signalling upregulated on both tumor and CIK cells upon engagement (110). Recent advances in technology have led to the development of novel gene therapies delivered through viral constructs which directly target cancer cells and exert cytolytic activity (118-120).

d. Clinical applications of combined cellular immunotherapy in GI cancer: Dendritic cell vaccines and cytokine-induced killer cells

Based on preclinical evidence showing synergistic interaction between dendritic and CIK cells, numerous trials have investigated the role of combined immunotherapy with DC vaccines and CIK cells. In general, these trials have confirmed preclinical observations for GI cancers with enhanced humoral immune responses translating into significantly improved ORR and survival rates (121-126). A recent meta-analysis including 70 trials has reported significant PFS (HR: 0.55, p<0.00001) and OS (HR:0.61, p<0.00001) advantage with DC/CIK therapy in CRC for all stages. Although direct comparison with single CIK therapy is not given, benefit rates in outcomes are numerically higher for the DC/CIK combination, providing clinical evidence of the synergistic activity (100).

IV. Case reports

Case 1

The sixty-six-year-old male patient was admitted to the clinic with epigastric pain, fatigue and exertional dyspnea. Laboratory findings revealed iron deficiency anemia, for which he was referred to the endoscopy unit for further evaluation. A subsequent gastroscopy showed an erosive mass measuring 3x4 cm. located in the antrum, whereas a colonoscopy denied any pathologic findings. A biopsy obtained from the antral mass confirmed the diagnosis as poorly cohesive adenocarcinoma, consistent with gastric primary. Following the completion of imaging workup which showed multiple regional enlarged lymph nodes without any systemic metastatic involvement, he was recommended to undergo perioperative platin-based chemotherapy followed by surgery. Nevertheless, he refused chemotherapy and opted for upfront surgery. Subsequently, he underwent distal subtotal gastrectomy on August 21, 2017. Pathologic evaluation revealed a poorly cohesive, signet-cell ring adenocarcinoma in the antral region which invaded the serosa as well as the lesser omentum and 32 out of 75 lymph nodes involved, consistent with T4N3M0 gastric cancer. Further analysis by FISH revealed Her2 amplification. At that stage, we discussed the risks and benefits of adjuvant chemotherapy, which he refused and opted for personalized cellular immunotherapy. Following regulatory approval based on encouraging evidence from a phase II prospective study combining adjuvant chemotherapy with a cancer vaccine and cytokine-induced killer cells in patients undergoing surgery with gastric cancer, he underwent a combined immunotherapy program consisting of dendritic cells pulsed with autologous tumor cells and cytokine-induced killer cell infusions intercalated with PD-1 inhibition, and metronomic oral cyclophosphamide for 6 months. The adoptive cell therapy was supported with an in-vivo cellular expansion protocol including rh-interleukin-2 and interferon-alpha injections. The detailed treatment protocol is depicted in Fig 1.
He received four DC/CIK infusions between Oct 10, 2017 and Feb 20, 2018, which was lower than the planned schedule due to side effects related to IL-2 injections and limited cellular product yield. In vivo expansion was generally provided by IFN-alpha as IL-2 had to be discontinued due to severe fever, chills, and arthralgias. Starting from the initiation of the treatment protocol, he was routinely screened with imaging studies every twelve weeks and remained disease-free until November 2018, when he started complaining of weight loss. A subsequent endoscopic evaluation revealed recurrent tumoral lesions at the distal anastomosis level extending throughout the colonic mucosa. Following confirmation of Her2 overexpressing adenocarcinoma consistent with relapsed gastric cancer, systemic chemotherapy with trastuzumab was initiated. Unfortunately, failing several lines of chemotherapy combined with Her-2 inhibition, he was deceased on November 2019 with leptomeningeal involvement.
Figure 1. Detailed treatment protocol.
Figure 1. Detailed treatment protocol.
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The protocol was initiated following 7 days of conditioning with metronomic oral cyclophosphamide 50 mg daily. Cellular therapy was initiated on day zero (0) with DC injections and CIK infusion. DC/CIK therapy was planned to be given weekly for 4 cycles, followed by maintenance infusions every 8 weeks. Four hours after completion of the infusion, an in-vivo expansion protocol with subcutaneous injections of rh-interleukin-2 (IL-2) at 3 mU/m2 and rh-IFN alpha (IFN-a) at 3 MU/m2 was initiated. IL-2 was planned to be given daily for 5 consecutive days (Days 0-4), whereas IFN-a was administered every other day as a single daily dose for 3 days (Days 0, 2, 4) after DC/CIK infusions. On day +2, nivolumab was started at a dose of 3 mg/kg given as an IV infusion over 60 minutes, followed by 30 minute-infusions at subsequent doses every 2 weeks. The initial plan was to continue the whole protocol for 6 months, and continue with maintenance DC/CIK boosters every 3 months for up to 2 years or progression; whichever occurs first. (Abbreviations: Cont’nd: continued; Rh: recombinant human)

Case 2

A 39-year-old male patient was admitted to our clinic in February 2019 with progressive metastatic right colon cancer. His diagnosis dated back to March 2017, when he underwent a right hemicolectomy for a tumor in the caecum. The pathologic evaluation was consistent with a T3N1M0 KRAS mutant, grade 2 mucinous adenocarcinoma. Following adjuvant chemotherapy with the FOLFOX regimen for 3 months, he was followed up until August 2017, when recurrence in the abdominal cavity and liver metastases were noted. He then underwent several cycles of perioperative chemotherapy consisting of oxaliplatin and capecitabine, followed by cytoreductive surgery with HIPEC, as well as radioablative treatment (RFA) to the liver metastases until November 2017. In January 2018 progression in the liver lesions was identified and he received combination chemotherapy with oxaliplatin-irinotecan-flurouracil and bevacizumab with consolidative RFA that was completed in March 2018. Nevertheless, due to rapid and subsequent recurrences, he underwent a second cytoreductive surgery with HIPEC in May 2018 and a colon resection in January 2019. He was then referred to our clinic for a second opinion on personalized immunotherapy strategies. Imaging studies obtained after his admission failed to show any recurrent lesions, consistent with minimal residual disease. After a thorough discussion of further treatment options, he decided to pursue a combined immunotherapy approach consisting of a personalized DC vaccine, CIK adoptive cell therapy, PD-1 inhibition and bevacizumab based on favorable responses reported by several phase II clinical trials. Following regulatory approval, he underwent apheresis by which cellular products were manufactured under GMP conditions. A detailed treatment plan is provided in Figure 2. He received this protocol between May 15, 2019 and Aug 22, 2019. On the day of the first DC/CIK administration, he experienced a grade 3 infusion reaction with fever, chills and hypotension which required hospitalization. The incident was managed with hydration as well as intravenous medications and did not recur on subsequent therapies. He generally tolerated the protocol well with minimal temporary fatigue throughout the treatment. Unfortunately, an abdominal MRI obtained in September 2019 revealed two implants consistent with peritoneal recurrence. He then went back to his primary treating center for continuation of systemic therapy. In February 2020, he reported being enrolled in a clinical trial with investigational agents which proved to be unresponsive and had been under evaluation for additional systemic therapy. He is still alive with disease progression as of July 2023.
Figure 2. Detailed treatment protocol.
Figure 2. Detailed treatment protocol.
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The protocol was initiated on day (-7) with a conditioning regimen consisting of intravenous cyclophosphamide given as a single IV infusion at 1 gr/m2, followed by subcutaneous GM-SCF injections at a dose of 75 mcg/day starting on day (-1) and continued for 5 days until day 3. Cellular therapy was initiated on day zero (0) with DC injections and CIK infusion. DC/CIK therapy was planned to be given twice every 2 weeks, followed by 4 cycles given every 4 weeks and maintenance infusions every 8 weeks thereafter for up to 2 years or progression; whichever occurs first. On day 7, nivolumab was started at a dose of 3 mg/kg given as an IV infusion over 60 minutes, followed by 30 minute-infusions at subsequent doses every 2 weeks in addition to bevacizumab at a dose of 5 mg/kg given every 2 weeks throughout the immunotherapy period. (Abbreviations: GM-CSF: granulocyte-macrophage-colony stimulating factor; CTX: cyclophosphamide)

Case 3

A 47-year-old patient was referred to our clinic for a second opinion on personalized immunotherapy options as consolidation treatment after induction chemotherapy and surgery for left-sided colon cancer in addition to chemoembolization for liver metastases. His initial diagnosis dated back to March 2018, when a tumor located in the rectosigmoid colon with synchronous multiple liver metastases was detected. After a stable response to three months of systemic chemotherapy with oxaliplatin, irinotecan and fluorouracil combined with cetuximab, he underwent radiofrequency ablation to several liver metastases on May 29, 2018, followed by a low anterior resection procedure with resection of residual liver metastases on June 25, 2018. The pathologic evaluation revealed a grade 2 adenocarcinoma with minimal response to cytotoxic chemotherapy. With no proven evidence for further chemotherapy, we discussed the potential benefits of a combination regimen with DC/CIK infusions administered in conjunction with programmed death-1 (PD-1) and vascular endothelial growth factor (VEGF) inhibitors, based on encouraging outcomes with personalized cellular immunotherapy strategies in the minimal residual disease setting. Following regulatory approval, he underwent apheresis by which cellular products were manufactured under GMP conditions. A detailed treatment plan is provided in Figure 3.
He received this protocol between Feb 15, 2019 and Apr 30, 2019, followed by nine booster DC/CIK infusions until Jan 13, 2021. He generally tolerated the protocol well, with no major toxicity throughout the treatment. Follow-up imaging studies failed to show any recurrence until July 4, 2022, when a nodular lesion in the right thoracic wall and an FDG-avid lymph node in the right hilar station were detected in a PET-CT scan. He then underwent resection of the thoracic lesion, which confirmed the diagnosis as metastatic adenocarcinoma, consistent with colorectal primary. A comprehensive genomic evaluation revealed pathogenic mutations in NRAS, TP-53, SMAD4, APC and ZFHX3 genes, and no PD-L1 expression. The tumor was microsatellite stable and had a low tumor mutation burden of 3.7 m/MB. He then underwent 6 cycles of systemic chemotherapy with the FOLFOX and bevacizumab combination between June and December 2022, which resulted in a partial regression of the hilar lymph node and no residual disease elsewhere. At this stage, we discussed further therapy options and based on his favorable experience with previous consolidative immunotherapy resulting in a sustained progression-free interval, we decided to pursue a similar immunotherapy strategy following irradiation of the residual lymph node basin. Based on the previous protocol, we initiated the treatment with the conditioning regimen on April 4, 2023, and he received this protocol until the last bevacizumab infusion which was administered on July 12, 2023. There were no major side effects encountered during the immunotherapy period except a prolonged grade 1 diarrhea accounted for by pembrolizumab and managed by symptomatic medications. Response assessment by a PET-CT scan obtained on July 24, 2023 failed to show any foci of active lesions suspicious of residual disease. He is planned to receive booster vaccinations every 2-3 months until the vaccine product is exhausted. Please refer to Fig 3 for details on the final immunotherapy protocol.
The protocol was initiated on day (-7) with a conditioning regimen consisting of intravenous cyclophosphamide given as a single IV infusion at 1 gr/m2, followed by subcutaneous GM-SCF injections at a dose of 75 mcg/day starting on day (-1) and continued for 5 days until day 3. Cellular therapy was initiated on day zero (0) with DC injections and CIK infusion. DC/CIK therapy was planned to be given every 2 weeks for 4 cycles, followed by 4 cycles given every 4 weeks and maintenance infusions every 8 weeks thereafter for up to 2 years or progression; whichever occurs first. On day 7, pembrolizumab was started at a fixed dose of 200 mg given as an IV infusion over 60 minutes, followed by 30 minute-infusions at subsequent doses every 2 weeks in addition to bevacizumab at a dose of 7.5 mg/kg given every 3 weeks throughout the immunotherapy period. Starting from the 3rd week after IV cyclophosphamide, oral metronomic cyclophosphamide at 50 mg daily was started and continued throughout. The second immunotherapy protocol was similar to the original protocol except that DC/CIK infusions were administered every 10 days (twice in a 3-week cycle), with omission for some DC vaccine injections and continued for 6 weeks after switching to every 3-week schedule for 6 more weeks due to limitations in cellular content obtained by apheresis. (Abbreviations: GM-CSF: granulocyte macrophage colony stimulating factor; CTX: cyclophosphamide)

Methods

All cellular therapy products were produced under GMP conditions in an approved facility. Dendritic cells and CIK cells were generated from peripheral blood mononuclear cells expanded ex vivo via incubation with cytokines including IL-1, IFN-alpha, TNF-alpha, and IFN-gamma. Activated mature dendritic cells were further incubated with autologous cancer cells to produce a personalized DC vaccine for each patient. Generally, seven days of culture was sufficent to produce the vaccine, whereas 3-4 weeks were required to produce a sufficient amount of CD3+/CD56+ CIK cells to ensure optimal activity. Although the number of viable cells differed for each apheresis procedure, the ultimate aim was to administer 1-10 x 109 cells at each administration. Dendritic cell vaccine pulsed with autologous tumor cells was split in aliquotes and administered subcutanously in all four limbs. Frozen CD3+/CD56+ CIK cells cells suspended in isotonic saline solution was thawed and administered as an IV infusion over 10-20 minutes.
All subjects gave their informed consent for inclusion before they participated in the study. The investigational study was conducted in accordance with the Declaration of Helsinki, and individual approvals were obtained by the Ethics Committee of Gayrettepe Florence Nightingale Hospital, Istanbul, Turkey and subsequently from the Ministry of Health, Health Services General Directorate, Department of Tissue, Organ Transplant and Dialysis (ID codes: 02.11.2017/56733164/203; 27.03.2019; no:216/56733164/203; 19.01.2023/E-56733164-203-210725859).

V. Future prospects and conclusion

Accumulating evidence suggests a possible beneficial role for active immunotherapy with cancer vaccines and adoptive cell therapy with CIK cells in combination with several immunomodulatory agents for GI cancers. Nevertheless, most of the trials evaluating these strategies suffer from a lack of standardization and imbalances in patient characteristics, which limit our ability to reach a definite conclusion on the optimal clinical use of cellular immunotherapy. Furthermore, both tumor and patient-related factors cause temporal immunologic plasticity over the evolution of disease progression through aberrant genomic signaling and TME modulation; which complicate the process even further. Given these constraints, our preliminary experience with cellular immunotherapy showing a lack of efficacy in two out of three patients despite the addition of VEGF and PD-1 inhibiton is in line with previous reports highlighting the difficulties in achieving an immune-mediated response in an otherwise “cold” tumor microenvironment seen in GI cancers. Nevertheless, in the patient with de novo metastatic CRC who had been refractory to initial chemotherapy, the progression-free interval of 18 months after completion of immunotherapy is encouraging and may be considered as a signal of activity. However, a limitation in our protocol that should be mentioned is the lack of laboratory analysis to monitor the humoral immunologic response generated throughout the treatment and follow-up period.
Cancer immunotherapy is a rapidly evolving field. Advances in technology have led to the development of engineered cellular systems or synthetic nanotechnology-based materials that tap into the immune system to activate cytotoxic and memory-effector response against cancer cells. Innovative delivery systems such as non-replicating viral particles, nanoliposomes, exosomes or polymeric nanoparticles loaded with immune checkpoint inhibitors, siRNA, or tumor antigens have been shown to hold great potential as immunotherapeutics by inducing potent anti-tumor response as well as modulating an immunologically “cold” TME as demonstrated by preclinical studies (89, 127-130). Furthermore, engineered dendritic cells expressing CD40 bispecific antibodies showing anti-cancer activity through enhanced DC and T cell activity, hold promise as vaccines against several cancers (131).
Oncolytic viruses which are specifically designed to selectively replicate in cancer cells, possess a potential for dual anti-tumor action by a direct cytolytic effect as well as activation of the stroma to induce an immunologic effector cell response. Engineered oncolytic constructs derived from herpes, adeno, vaccinia, pox or reoviruses have been evaluated in several phase I trials, showing feasibility of this approach with manageable side effects as well as humoral and genomic evidence cancer-specific immune activity such as cytotoxic CD8 (+) and CD4 (+) Th1 cell infiltration; decreased VEGF and tumor-promoting miRNA levels in tumor samples and upregulated IFN-gamma and IL 12 in blood samples (132-134). However, a phase II randomized trial including 103 patients with metastatic CRC failed to show a benefit of the oncolytic Pelareorep, a reovirus construct, when combined with the standard FOLFOX and bevacizumab regimen, with a shorter PFS in the investigational arm (9 vs 7 months, HR: 1.59, p: 0.046), despite a higher ORR (HR: 2.52; p: 0.03) (135). Recently, a phase II study investigating a more contemporary approach with dual PD-1 and CTLA blockade in combination with a vaccinia virus-based oncolytic immunotherapy (Pexastimogene devacirepvec) targeting aberrant EGFR/RAS pathway was reported. The study cohort comprising 34 patients with refractory MSS metastatic CRC was split into two groups receiving single or dual checkpoint inhibition. Although there was no significant difference noted between the two regimens, there was evidence of increased peripheral cytotoxic T cell activity (136). Similarly, Enadenotucirev, an adenovirus-based oncolytic construct was investigated in combination with nivolumab in 51 patients with refractory tumors, 35 of whom had metastatic CRC. Despite the failure to achieve a meaningful objective response, an encouraging survival rate coupled with increased intra-tumoral CD8 (+) T cell activity was observed (137). Further improved activity was reported in a preclinical study investigating the role of herpes simplex-based oncolytic virus combined with PD-1 blockade and trametinib, a novel agent targeting the MAPK-KRAS signaling pathway in a murine model with KRAS or BRAF mutant tumors (138). The results of ongoing trials with engineered constructs encoding multiple tumor- and immune cell-related epitopes in combination with immunomodulatory strategies are awaited with enthusiasm.
Chimeric antigen receptor T cells (CAR-T) have revolutionized adoptive immunotherapy by sustained complete remissions achieved in patients with leukemias (139). There exists encouraging evidence from preclinical and early phase I clinical trials evaluating the role of CAR-T or NK cells engineered to express specific immunomodulatory molecules to overcome resistance in hematologic malignancies (140-142). Nevertheless, the promise of CAR-T’s has not been realized for solid tumors and several hurdles remain to be faced until meaningful clinical utility can be established. Spatial and temporal heterogeneity in tumor antigen expression, as well as obstacles to infiltrating tumor masses protected from immunologic cell kill through abnormal vasculature and a hostile TME are the major factors that pose barriers to an efficacious CAR-T cell therapy (143). Energetic efforts have been placed to improve CAR-T activity in solid tumors by building novel cellular constructs engineered to express immunostimulatory checkpoints including CD28 and OX-40 (144), specific tumor antigens (145-147) or effector cytokines such as IL-12, IL-18 (148). These strategies have yielded promising efficacy in murine models engrafted with several tumors like melanoma, mesothelioma, neuroendocrine tumors as well as CRC (149). However, there exists evidence from phase I clinical trials showing limited activity with short-acting stable disease as the best response, highlighting the challenges that need to be addressed by future research (150). The Keynote B79 trial, which is an ongoing study evaluating the role of an allogeneic CAR-T cell expressing an NKG2D receptor targeting both tumor cells and the TME presents a valuable example of where the field is directed. Based on previous experience suggesting preliminary efficacy with this engineered construct aiming to induce an MHC-I unrestricted immune-mediated cell kill and to antagonize immune suppressive components of the TME, the trial combines complementary immunomodulating strategies including a chemotherapy backbone with FOLFOX and pembrolizumab to drive a deeper and sustained response in a cohort of patients with refractory CRC (151).
Advances in the field of immunotherapy have led to the accumulation of an expanding pool of information on the complexity of anti-cancer immunity. Despite encouraging results investigating combinations of innovative strategies as outlined above, clinical outcomes in GI cancers have proved to pose challenges with regards to immunotherapy. In fact, preclinical and translational analyses have led to the identification of a substantial number of biomarkers, which have been implicated to play crucial roles in the generation of the immunosuppressant stroma, an inherent characteristic encountered in most GI cancers. Future studies focusing on the identification of the genomic characteristics underlying the hostile “cold” TME, as well as mechanisms elucidating the intricate interactions between effector and suppressor cell components of the tumor stroma will help to define patients who would derive benefit from these approaches. These ongoing efforts will undoubtedly deepen our understanding of the immunologic landscape and provide unprecedented insight into optimizing personalized immunotherapy for patients with GI cancers.

Author Contributions

Conceptualization, Methodology, Formal Analysis, Investigation, Resources, Data Curation: Y.E. and U.A; Writing – Original Draft Preparation; Writing – Review & Editing; Visualization, Supervision, Funding Acquisition: Y.E.

Conflicts of Interest

The authors declare no conflict of interest relevant to this work.

Informed Consent; Institutional and Central Review Board Approval

All subjects gave their informed consent for inclusion before they participated in the study. The investigational study was conducted in accordance with the Declaration of Helsinki, and individual approvals were obtained by the Ethics Committee of Gayrettepe Florence Nightingale Hospital, Istanbul, Turkey and subsequently from the Ministry of Health, Health Services General Directorate, Department of Tissue, Organ Transplant and Dialysis (ID codes: 02.11.2017/56733164/203; 27.03.2019; no:216/56733164/203; 19.01.2023/E-56733164-203-210725859).

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Figure 3. Detailed treatment protocol.
Figure 3. Detailed treatment protocol.
Preprints 84965 g003
Table 1. Selected clinical trials with cancer vaccines in gastric and esophageal cancers.
Table 1. Selected clinical trials with cancer vaccines in gastric and esophageal cancers.
Vaccine type Intervention/ neoantigen Adjuvant / Combined Tx Stage Design Outome measures Status Phase Reference
B-cell & Monocyte BVAC-B
(Her-2)
none IV Her2 (+) GC, 1   p r i o r   l i n e s   C T 0,4,8, 12 wks Establish MTD, AE completed Ib NCT03425773
Cellid Co, Ltd.
Allogeneic K562-GM Cyclophosphamide
Celecoxib
NED/MRD
Primary site
Esophagus & Mediastinal after standard Tx;6 vaccines q4 wks AE, humoral immune response Terminated (futility) Ib NCT01143545
NCI
mRNA personalized none IV Esophageal, GC, CRC; 1   p r i o r   l i n e s   C T ;
4 vaccines SC
AE, DCR, PFS, TTP, OS Unknown Ib NCT03468244
Chanhai Hospital, China
B-cell epitope Her-2 CT
(Cisplatin-FU/Capecitabine)
IV Her2 (+) GC/GE
D1,14,28 vaccine vs CT + vaccine
AE, ORR, humoral immune response Ongoing Ib NCT05315830
Bengbu Med College, China
MVB-BN
non-replicating viral
TAEK-VAC-Herby encoding
Her-2, CD40L, TF Brachyury
Trastuzumab
Pertuzumab
IV Her2 (+) GC/GE, Breast, Chordoma
3 vaccines
q3 wks
DLT Ongoing Ib/II NCT04246671
Bavarian Nordic
T-cell receptor KK-LC1 neoantigen
HLA-A-01:01 restricted
2 x prior CT & IL-2 4 days & Cyclophosphamide (D-6, -5) + Fludarabine
(D-6—2)
IV KK-LC1 (+) GC, breast, lung cancer
1 vaccine infusion
DLT Ongoing I NCT05035407
NCI
mRNA PGV-002
Personalized mRNA
PD-1/PD-L1 (expansion phase) IV GC, esophageal, liver cancer refractory to standard CT AE, ORR, MTD, PFS Ongoing I NCT05192460
Chinese Academy of MMS
NeoCura
Peptide Personalized Pembrolizumab, Cyclophosphamide (D-3), GM-CSF (D 1,4,8, 15) q 3 wks III -IV GC/GEJ, breast, NSCLC, HCC, Merkel, GU AE, ORR, feasibility, humoral immune response Ongoing I NCT05269381
Mayo Clinic
Peptide
(Da VINci)
OTSGC-A24
Multiple epitope
Nivolumab, Ipilimumab IV GC; refractory AE, ORR, humoral immune response Ongoing Ib NCT03784040
Natl. Uni Hospital, Singapore
Peptide iNEO-Vac-P01
Personalized
GM-CSF II-III
maintenance
Esophageal, resectable, after resection & (neo)/adj CT+PD-1
7 vaccines
D1-82
AE, RFS, OS, QoL Ongoing I ZheJiang Uni; Hangzhou Neoantigen Ther Co, Ltd
mRNA Personalized none IIIC -IV Esophageal ca; NSCLC
Failure of standard Tx
AE, ORR, TTP, PFS Ongoing NA NCT03908671
Zhengzhou Uni;
Stemirna Ther
Adenoviral
Self-replicating mRNA
GRT-C901 & R902
(predicted multiple epitopes)
Nivolumab
Ipilimumab
IV GE/G ≤ 1 prior CT
AE, ORR, dose finding Ongoing Ib NCT03639714
Gritstone Bio, Inc
(Abbreviations: HLA: human leucocyte antigen; Tx: treatment; CT: chemotherapy; GM-CSF: granulocyte macrophage colony stimulating factor; FU: 5-fluorouracil; NED: no evidence of disease; MRD: minimal residual disease; D: day; wks: weeks; GC: gastric cancer; CRC: colorectal cancer; NSCLC: non-small cell lung cancer; HCC: hepatocellular carcinoma; GU; genitourinary carcinoma; SC: subcutaneous; neo/adj CT: neoadjuvant / adjuvant chemotherapy; PD-1; programmed death-1; MTD: maximal toxic dose; AE: adverse effects; DCR: disease control rate; PFS: progression-free survival; RFS: relapse-free survival; TTP: time to progression; OS: overall survival; ORR; overall response rate; DLT: dose limiting toxicity; QoL: quality of life).
Table 2. Selected clinical trials with cancer vaccines in colorectal cancer.
Table 2. Selected clinical trials with cancer vaccines in colorectal cancer.
Vaccine type Intervention/ neoantigen Adjuvant / Combined Tx Stage Design Outome measures Status Phase Reference
Adenoviral
Self-replicating mRNA
GRT-C901 & R902
(predicted multiple epitopes)
Nivolumab
Ipilimumab
IV CRC-MSS; GC/GEJ; NSCLC; GU 1 prior CT AE, ORR, dose finding,
Humoral immune response
Completed Ib/II NCT03639714
Gritstone Bio, Inc
Adenoviral
Self-replicating mRNA
GRT-C901 & R902
(predicted multiple epitopes)
Atezolizumab
Ipilimumab
II-III; MRD (ctDNA (+)) CRC-MSS; Resected, after adj CT;
6 x vaccine
2x Ipilimumab; 13x Atezolizumab q4 wks
AE, ORR (ctDNA); RFS, OS) Terminated; reprioritization II NCT05456165
Gritstone Bio, Inc
Dendritic cell Personalized
Antigen pulsed DC
mFOLFOX6 IV Untreated mCRC;
CT vs CT+vac
Vaccination in cycles 1-3; 7-9
PFS, OS, ORR Unknown III NCT02503150
Second MMU, China
Adenovirus QUILT-2.004
Ad-CEA
Avelumab
CT (FOLFOX)
Bevacizumab
IV Untreated mCRC-MSS; CT (FOLFOX+Bevacizumab) vs CT+ Avelumab + vaccine x 12 PFS Terminated;
Futility on interim analysis
II NCT03050814
NCI
Viral (modified vaccinia Ankara- Bavarian Nordic) CV 301
MVA-BN CEA/MUC1
& Fowlpox booster
Bifunctional fusion protein composed of IgG1 PD-L1 & TGF-beta;
IL-15 fusion protein
IL-12
IV mCRC; small bowel ca. 2 p r i o r   l i n e s   C T
Triple vs quadruple Tx (± IL12)
AE, ORR; PFS; OS Completed II NCT04491955
NCI
Autologous Cryovax;
Personalized autologous tumor
Bioengineered allogenic immunecells (AlloStim) IV >2 previous lines of CT;
Vaccine x6 over 10 wks
DLT, QoL,
Humoral immune response; ORR
Completed II NCT02380443
Immunovative Therapies Ltd.
Allogenic engineered GVAX whole tumor cell engineered to secrete GM-CSF Cyc
Guadecitabine
IV mCRC; stable on 1-2nd Line CT;
Vaccine x 1 q 4 wks
AE; TIL; PFS Completed I NCT01966289
Sydney Kimmel Cancer Center
Peptide PolyPEPI1018
Six sythetic CTA neoantigens
TAS 102 IV mCRC-MSS; 2 prior CTVaccine q2 weeks x 7 doses AE, PFS, ORR; OS Completed I NCT05130060
Mayo clinic
mRNA mRNA 5671/V941-001 Pembrolizumab IV mCRC- KRASm-MSS; NSCLC; Pancreas
Vaccine q 3 wks 9 doses vs Vaccine + Pembrolizumab
AE, DLT Completed I NCT03948763
Merck Sharp & Dohme LLC
Synthetic peptide Her-2 & CEA
HLA A2 / A3 restricted
GM-CSF
Tetanus toxioid
Montanide ISA 51
II-IV CRC AE; T cell response in lymph node Terminated
Slow accrual
I NCT00091286
University of Virginia
Alphavirus replicon particles AVX 701
CEA
none III CRC;
After adjuvant CT
Vaccine x4
q 3 wks
AE; Humoral immune response Completed I NCT01890213
Duke University
Inactivated virus Influenza vaccine none Early, operable CRC; intratumoral injection x 1 AE, local immune responses Completed II NCT04591379
Zealand University
Plasmid DNA MYPHISMO
Tet-MYB
BGB-A317 (Anti-PD-1 IgG4 mab) IV mCRC, ACC;
refractory;
Vaccine x 6
q 7d
AE, ORR; CBR, PFS Completed I NCT03287427
Peter Mc Callum Cancer Centre, Au
Adenoviral
Self-replicating mRNA
GRANITE-GRT-C901 & R902
(predicted multiple epitopes)
Atezolizumab
Ipilimumab
5-FU
Bevacizumab
IV mCRC; maintenace following 1st L CT (FOLFOX-Bevacizumab) Molecular response;
PFS
Ongoing II/III NCT05141721
Gritstone Bio; Inc
Liposomal StimVax;
MUC-1
AlloStim;
Allogenic immune cells
IV mCRC-MSS;
>2 previous lines of CT;
Vaccine 3 cycles (x 5/wk q 6 wks)
AE, OS Ongoing IIb NCT04444622
Immunovative Ther, LTD
Dendritic cell Personalized neoantigen Nivolumab IV; MRD mCRC; resected liver met; HCC
Vaccine x 10 doses
q 2wks
RFS, Humoral immune response Ongoing II NCT04912765
Natl Cancer Centre, Singapore
Peptide Personalized synthetic neoantigen Pembrolizumab
Imoquimod
Sotigolimab
mCRC; any line; Pancreas ca;
Vaccine x 7-11 dose
q 2 wks
AE, Feasibility, ORR; PFS; OS Ongoing I NCT02600949
MD Anderson Cancer Center
Chimeric recombinant protein;
Recombinant viral
KISIMA-01;
ATP-128-Three neoantigens+
TLR agonist;
VSV-GP128-booster
Ezabenlimab (anti-PD-1) IV mCRC-MSS;
1st Line CT; refractory; liver-only
AE, PFS;
ORR; MTD; RFS
Ongoing I/II NCT04046445
Amal Ther.
Peptide KRAS peptide Nivolumab
Ipilimumab
IV mCRC; pancreas;
>2 previous lines of CT;
Vaccine x 3 q 7 d;
x 5 boosters q 8 wks
AE; humoral immune response; DFS; ORR; PFS; OS Ongoing I/II NCT04117087
Sydney Kimmel Cancer Center
Peptide PolyPEPI1018
7 peptide noeantigens;
Montanide
Atezolizumab IV mCRC-MSS
>2 previous lines of CT;
AE, ORR; PFS; OS;
Humoral immune response
Ongoing II NCT05243862
Treos Bio Ltd.
Yeast cell particles
PalloV-CRC;
Allogenic tumor cells delivered on yeast cell particles
none I-IV CRC prior to surgery
Vaccine x 4 q 4 wks
AE; humoral immune response Ongoing I NCT03827967
Cancer Insight, LLC
Dendritic cell COREVAX-1;
Dendritic cells pulsed with autologous tumor cells
IL-2 (D3-7) IV; MRD mCRC; Following resection AE; humoral immune response;
RFS; OS
Ongoing II NCT02919644
Instituto Scientifico Romagnolo per lo Studio e la cura dei Tumori
Peptide CLAUDE; EO2040; TAA-CD8 / CD4 T cell epitopes
Montanide
Nivolumab
II-IV; MRD (ct-DNA (+) mCRC; Following resection & standard Tx ORR at 6 months; AE; DFS; OS Ongoing II NCT05350501
Enterome
Lipid conjugated oligonucleotide & peptide ELI-002;
KRAS / NRAS
none MRD; ct-DNA (+) mCRC; pancreatic; NSCLC;
Vaccine x 4 q 7 d;
x 8 boosters/4 wks
Ct-DNA clearance; RFS; OS Ongoing I/II NCT05726864
Elicio Ther
Adenovirus Tri-AD5 Trivalent CEA/MUC-1/Brachyury nogapendekin alfa inbakicept (IL-15 agonist-fusion protein) Prevention Lynch Syndrome; colon adenomas & CRC NED;
Vaccine x 4
(wks 0,4,8,52)
Cumulative incidence of adenomas; extracolonic carcinomas; humoral immune responses Ongoing IIb NCT05419011
NCI
Adenovirus Nous-209;
Gad-209-FSP priming;
MVA-209-FSP booster
None Prevention gLynch Syndrome; NED after non-sporadic MMRd malignant tumors;
Vaccine D1 / booster wk 8
AE; humoral immune responses; response in colorectal adenomas; incidence of Lynch-associated carcinomas Ongoing I/II NCT05078866
NCI
(Abbreviations: DC: dendritic cell; CEA: carcinoembryonic antigen; MUC: mucin; KRAS: kirsten rat sarcoma virus; NRAS: neuroblastoma ras viral oncogene homolog; Tx: treatment; CT: chemotherapy; GM-CSF: granulocyte macrophage colony stimulating factor; FOLFOX: oxaliplatin-5-fluorouracil infusion-leucovorin; wk: week; d: days; IL: interleukin; NED: no evidence of disease; MRD: minimal residual disease; ct-DNA (+): circulating tumor DNA; wks: weeks; GC: gastric cancer; GEJ: cancer involving distal osephageal; cardia; proximal gastric areas; CRC: colorectal cancer; mCRC : metastatic CRC; CRC-MSS: microsatellite stable CRC; NSCLC: non-small cell lung cancer; HCC: hepatocellular carcinoma; GU; genitourinary carcinoma; gLynch: germline Lynch; MMRd: mismatch repair deficient; SC: subcutaneous; neo/adj CT: neoadjuvant / adjuvant chemotherapy; PD-L1; programmed death ligand-1; MTD: maximal toxic dose; AE: adverse effects; CBR: clinical benefit rate; DCR: disease control rate; PFS: progression-free survival; RFS: relapse-free survival; TTP: time to progression; OS: overall survival; ORR; overall response rate; DLT: dose limiting toxicity; QoL: quality of life).
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