Kildeeva, N.; Sazhnev, N.; Drozdova, M.; Zakharova, V.; Svidchenko, E.; Surin, N.; Markvicheva, E. Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin. Technologies2023, 11, 146.
Kildeeva, N.; Sazhnev, N.; Drozdova, M.; Zakharova, V.; Svidchenko, E.; Surin, N.; Markvicheva, E. Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin. Technologies 2023, 11, 146.
Kildeeva, N.; Sazhnev, N.; Drozdova, M.; Zakharova, V.; Svidchenko, E.; Surin, N.; Markvicheva, E. Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin. Technologies2023, 11, 146.
Kildeeva, N.; Sazhnev, N.; Drozdova, M.; Zakharova, V.; Svidchenko, E.; Surin, N.; Markvicheva, E. Approaches to Obtaining Water-Insoluble Fibrous Matrices from Regenerated Fibroin. Technologies 2023, 11, 146.
Abstract
Silk fibroin (SF) extracted from Bombyx mori cocoons is a biocompatible and biodegradable polymer shows potential as matrices for tissue engineering and regenerative medicine, or con-trolled drug delivery systems. Regenerated fibroin, obtained by removing sericin from natural silk, is water-soluble. Its aqueous solutions can be processed into a variety of materials, for example, fibrous matrices, using the electrospinning method.
In this article, the concentration dependences of the properties of aqueous solutions of regener-ated fibroin, that determine the possibility of obtaining electrospun mats for tissue-engineering applications, are studied. A slowdown in the growth of viscosity after a concentration of fibroin of 30% and a decrease in the transparency of the solution were found; this may be due to the on-set of conformational rearrangements: the transition of fibroin from the random coils, charac-teristic for dilute solutions, to the anisotropic conformation of the helix and the appearance of β-pleated sheets. Fibrous mats from regenerated fibroin were obtained from solutions with a concentration of 10 and 20%.
Two methods were used to prevent solubility of fibroin-based matrices: conversion of fibroin to the β-conformation by treatment with an ethanol solution and chemical cross-linking with gen-ipin (Gp). The structures of the fibrous samples were studied by AFM and confocal laser scan-ning microscopy. The composition of the moulding solution for the electrospinning of regener-ated fibroin fibrous matrices and the conditions for their modification have been optimised. It has been shown that the composition of fibroin with chitosan contributes to a decrease in the solubility of electrospun fibrous matrices by increasing the number of amino groups available for modification by Gp, and also leads to a decrease in the diameter of the fibers.
Electrospun fiber matrices based on regenerated SF, modified by cross-linking with Gp in water-alcohol solutions, promote cell growth and proliferation and may be promising for tissue engineering.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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