Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Meat Juice and Oral Fluid as Alternative to Serum for Aujeszky Disease Monitoring in Pigs

Emanuele Carella
* ORCID logo ,
Claudio Caruso
,
Ana Moreno Martin
ORCID logo ,
Alessia Di Blasio
,
Francesca Oberto
,
Nicoletta Vitale
* ORCID logo ,
Loretta Masoero
Version 1 : Received: 4 September 2023 / Approved: 4 September 2023 / Online: 6 September 2023 (14:54:05 CEST)

A peer-reviewed article of this Preprint also exists.

Carella, E.; Caruso, C.; Moreno, A.; Di Blasio, A.; Oberto, F.; Vitale, N.; Masoero, L. Meat Juice and Oral Fluid as Alternatives to Serum for Aujeszky Disease Monitoring in Pigs. Microorganisms 2023, 11, 2418. Carella, E.; Caruso, C.; Moreno, A.; Di Blasio, A.; Oberto, F.; Vitale, N.; Masoero, L. Meat Juice and Oral Fluid as Alternatives to Serum for Aujeszky Disease Monitoring in Pigs. Microorganisms 2023, 11, 2418.

Abstract

Aujeszky Disease Virus (ADV) is a double-stranded DNA virus with a lipoprotein envelope. The natural hosts of the infection are suidae, but the virus can infect many other mammals. The gold-standard method identified by the WOAH for the diagnosis of Aujeszky disease is ELISA method. The objective of this study was to compare the performance of meat juice and oral fluid matrices using a commercial ELISA kit designed for serum. A total of 80 blood and oral fluid samples were collected from four pig farms selected for this study. Diaphragm muscle samples of about 100 g and blood samples were collected from 213 animals at the abattoir. These biological matrices were collected from the same animals and tested using a competitive ELISA kit to detect antibodies against ADV. The relative accuracy of the meat juice compared to that of the serum was 96.7% (95% CI: 93.3%-98.7%), with 206 correct results out of 213. The relative accuracy of the oral fluid compared to that of the serum was 61.3% (95% CI: 49.7%-71.9%), with 58 correct results out of 80. Meat juice has a better combination of sensitivity and specificity than oral fluid. The usage of meat juice in routine diagnostic examinations could be achieved after further investigations to optimize the procedure.

Keywords

Meat juice; oral fluid; Aujeszky disease virus; ELISA; pigs

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.