Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Responsiveness of Candidate Genes on CoPv01CDRK/PhgPv01CDRK Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens

Maike Lovatto
ORCID logo ,
Maria Celeste Gonçalves-Vidigal
* ,
Mariana Vaz Bisneta
,
Alexandre Catto Calvi
,
Josmar Mazucheli
,
Pedro Soares Vidigal Filho
,
Eduardo Gomes Rosa Miranda
,
Maeli Melotto
Version 1 : Received: 31 August 2023 / Approved: 4 September 2023 / Online: 5 September 2023 (04:41:27 CEST)

A peer-reviewed article of this Preprint also exists.

Lovatto, M.; Gonçalves-Vidigal, M.C.; Vaz Bisneta, M.; Calvi, A.C.; Mazucheli, J.; Vidigal Filho, P.S.; Miranda, E.G.R.; Melotto, M. Responsiveness of Candidate Genes on CoPv01CDRK/PhgPv01CDRK Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens. Int. J. Mol. Sci. 2023, 24, 16023. Lovatto, M.; Gonçalves-Vidigal, M.C.; Vaz Bisneta, M.; Calvi, A.C.; Mazucheli, J.; Vidigal Filho, P.S.; Miranda, E.G.R.; Melotto, M. Responsiveness of Candidate Genes on CoPv01CDRK/PhgPv01CDRK Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens. Int. J. Mol. Sci. 2023, 24, 16023.

Abstract

Anthracnose (ANT) and angular leaf spot (ALS) are significant diseases in common bean, leading to considerable yield losses under specific environmental conditions. The California Dark Red Kidney (CDRK) bean cultivar is known for its resistance to multiple races of both pathogens. Previous studies have identified the CoPv01CDRK/PhgPv01CDRK resistance loci on chromosome Pv01. Here, we evaluated the expression levels of ten candidate genes near the CoPv01CDRK/PhgPv01CDRK loci and plant defense genes using quantitative real-time PCR in CDRK cultivar inoculated with races 73 of Colletotrichum lindemuthianum and 63-39 of Pseudocercospora griseola. Gene expression analysis revealed that the Phvul.001G246300 gene exhibited the most elevated levels, showing remarkable 7.8-fold and 8.5-fold increases for ANT and ALS, respectively. The Phvul.001G246300 gene encodes an abscisic acid (ABA) receptor pyrabactin resistance - PYR1-like (PYL) protein, which plays a central role in crosstalk between ABA and jasmonic acid responses. Interestingly, our results also showed that the other defense genes were initially activated. These findings provide critical insights into the molecular mechanisms underlying plant defense against these diseases and could contribute to the development of more effective disease management strategies in the future.

Keywords

CoPv01CDRK/PhgPv01CDRK loci; candidate gene expression; common bean-anthracnose interaction; common bean-angular leaf spot interaction; plant defense genes

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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