Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Optimization of Se- and Zn-Enriched Mycelium of Lentinula edodes (Berk.) Pegler as a Dietary Supplement with Immunostimulatory Activity

Małgorzata Kałucka
,
Aleksander Roszczyk
,
Marzenna Klimaszewska
,
Beata Kaleta
ORCID logo ,
Ewelina Drelich
,
Anna Błażewicz
,
Sandra Górska-Jakubowska
ORCID logo ,
Eliza Malinowska
,
Marek Król
,
Aleksandra Maria Prus
,
Katarzyna Trześniowska
,
Aleksandra Wołczyńska
,
Przemysław Dorożyński
,
Radoslaw Zagozdzon
ORCID logo ,
Jadwiga Turło
* ORCID logo
Version 1 : Received: 31 August 2023 / Approved: 31 August 2023 / Online: 1 September 2023 (13:00:45 CEST)

A peer-reviewed article of this Preprint also exists.

Kałucka, M.; Roszczyk, A.; Klimaszewska, M.; Kaleta, B.; Drelich, E.; Błażewicz, A.; Górska-Jakubowska, S.; Malinowska, E.; Król, M.; Prus, A.M.; Trześniowska, K.; Wołczyńska, A.; Dorożyński, P.; Zagożdżon, R.; Turło, J. Optimization of Se- and Zn-Enriched Mycelium of Lentinula edodes (Berk.) Pegler as a Dietary Supplement with Immunostimulatory Activity. Nutrients 2023, 15, 4015. Kałucka, M.; Roszczyk, A.; Klimaszewska, M.; Kaleta, B.; Drelich, E.; Błażewicz, A.; Górska-Jakubowska, S.; Malinowska, E.; Król, M.; Prus, A.M.; Trześniowska, K.; Wołczyńska, A.; Dorożyński, P.; Zagożdżon, R.; Turło, J. Optimization of Se- and Zn-Enriched Mycelium of Lentinula edodes (Berk.) Pegler as a Dietary Supplement with Immunostimulatory Activity. Nutrients 2023, 15, 4015.

Abstract

Mycelial cultures of Lentinula edodes, an edible and medicinal mushroom, have been used in our previous research to obtain selenium-containing immunomodulatory preparations. Our current attempts to obtain a new preparation containing both selenium and zinc, two micronutrients necessary for the functioning of the immune system, extended our interest in the simultaneous accumulation of these elements by mycelia growing in media enriched with selenite and zinc(II) ions. Subsequently, we have studied the effects of new L. edodes mycelium water extracts with different proportions of selenium and zinc concentrations on activation of T cell fraction of human peripheral blood mononuclear cells (PBMCs). Flow cytometry analysis was used to measure the expression of activation markers on human CD4+ and CD8+ T cells stimulated by anti-CD3 and anti-CD3/CD28 antibodies (Abs). It was demonstrated that all extracts with different proportions of polysaccharides, selenium and zinc tended to upregulate the expression of all activation markers on both types of T cell populations stimulated in parallel with anti-CD3/anti-CD28 beads, however, statistically significant changes were observed for PD-1 and CD25 antigens on CD8+ T cells. The selenium and zinc content in the examined preparations modified the immunomodulatory activity of mycelial polysaccharides, however, the mechanisms of action of various active ingredients of the mycelial extracts seem to be different.

Keywords

zinc–selenite interactions; polysaccharides; L. edodes; immunomodulation

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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