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A peer-reviewed article of this preprint also exists.
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp) | no. injected embryos | larval hatch rate (%) | adult eclosion rate (%) | total no. G0 families | % fertile G0 families | no. transg. G0 families | total no. G1 screened | no. transg. events | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | pBac | 300/150 | AH452 * | (5267) 8649 | 623 | 29.21 | 78.57 | 8 | 100.00 | 4 | n.d. | 4 | 2.80 |
2 | pBac | 200/500 | V3 | (3545) 6911 | 234 | 31.20 | 86.30 | 25 | 52.00 | 1 | 8830 | 1 | 1.25 |
3 | pBac | 400/600 | V285 | (5180) 8682 | 705 | 25.82 | 64.29 | 18 | 88.89 | 2 | 14966 | 2 | 1.71 |
4 | pBac | 300/500 | V286 | (5600) 9102 | 680 | 13.38 | 41.76 | 15 | 60.00 | 0 | 8772 | 0 | 0.00 |
5 | pBac | 160/185 | V258 | (6190) 9690 | 922 | 10.41 | 37.50 | 18 | 83.33 | 0 | 6631 | 0 | 0.00 |
6 | pBac | 400/600; 228/342 | V258 | (6190) 9690 | 599 | 24.21 | 34.48 | 11 | 100.00 | 0 | 18721 | 0 | 0.00 |
7 | pBac | 300/150 | V257 | (7088) 10587 | 1113 | 42.59 | 76.37 | 26 | 100.00 | 2 | 93998 | 2 | 0.55 |
8 | pBac | 200/500 | V19 | (3630) 7163 | 257 | 18.29 | 63.83 | 9 | 100.00 | 2 | 5185 | 2 | 6.67 |
9 | pBac | 300/500 | V19 | (3630) 7163 | 192 | 33.85 | 61.54 | 11 | 63.64 | 0 | 2679 | 0 | 0.00 |
10 | pBac | 200/500 | V19 | (3630) 7163 | 399 | 13.53 | 61.11 | 13 | 46.15 | 0 | 1313 | 0 | 0.00 |
11 | pBac | 200/500 | V19 | (3630) 7163 | 462 | 14.72 | 48.53 | 7 | 100.00 | 2 | 1389 | 2 | 6.06 |
12 | pBac | 300/300 | V368 | (5835) 11097 | 257 | 5.84 | 60.00 | 7 | 42.86 | 0 | 502 | 0 | 0.00 |
13 | pBac | 100/300 | V368 | (5835) 11097 | 148 | 5.41 | 62.50 | 4 | 75.00 | 0 | 382 | 0 | 0.00 |
avg | 1.46 | ||||||||||||
14 | HypB | 100/200 | V286 | (5600) 9102 | 594 | 8.08 | 75.00 | 12 | 75.00 | 0 | 9382 | 0 | 0.00 |
15 | HypB | 200/200 | V286 | (5600) 9102 | 730 | 8.63 | 79.37 | 17 | 70.59 | 0 | 7604 | 0 | 0.00 |
16 | HypB | 200/200 | V258 | (6190) 9690 | 895 | 6.82 | 65.57 | 13 | 69.23 | 0 | 5199 | 0 | 0.00 |
avg | 0.00 |
Exp. no. | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp) | no. injected eggs | no. hatched larvae | hatch rate (%) | no. fertile adults | fertile eclosion rate (%) | no. transg. lines | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
Ds 1 | pBac | 200/500 | AH443 | (9191) 12576 | 75 | n. d. | n. d. | 25 | n.d. | 4 | 16.00 |
Ds 2 | pBac | 200/500 | V220 | (7865) 11249 | 1601 | 231 | 14.43 | 65 | 28.14 | 1 | 1.54 |
Ds 3 | pBac | 200/500 | V221 | (7867) 11252 | 443 | 102 | 23.02 | 9 | 8.82 | 0 | 0.00 |
Ds 4 | pBac | 200/500 | V146 | (7118) 10503 | 481 | 173 | 35.97 | 20 | 11.56 | 2 | 10.00 |
Ds 5 | pBac | 200/500 | V183 | (7583) 10968 | 753 | 305 | 40.50 | 43 | 14.10 | 5 | 11.63 |
Ds 6 | pBac | 200/500 | V184 | (9438) 12823 | 640 | 167 | 26.09 | 17 | 10.18 | 0 | 0.00 |
Ds 7 | pBac | 200/500 | V185 | (8493) 11878 | 802 | 346 | 43.14 | 53 | 15.32 | 6 | 11.32 |
Ds 8 | pBac | 200/500 | V188 | (10347) 13732 | 631 | 285 | 45.17 | 55 | 19.30 | 1 | 1.82 |
Ds 9 | pBac | 200/500 | V213 | (10059) 13443 | 538 | 107 | 19.89 | 27 | 25.23 | 0 | 0.00 |
Ds 10 | pBac | 200/500 | V215 | (8204) 11589 | 626 | 173 | 27.64 | 29 | 16.76 | 1 | 3.45 |
Ds 11 | pBac | 200/500 | V226 | (8163) 12054 | 410 | 51 | 12.44 | 9 | 17.65 | 1 | 11.11 |
Ds 12 | pBac | 200/500 | V227 | (10018) 13909 | 493 | 122 | 24.75 | 12 | 9.84 | 1 | 8.33 |
Ds 13 | pBac | 200/500 | V228 | (8607) 12498 | 310 | 97 | 31.29 | 13 | 13.40 | 0 | 0.00 |
Ds 14 | pBac | 200/500 | V250 | (9072) 12963 | 378 | 129 | 34.13 | 28 | 21.71 | 0 | 0.00 |
Ds 15 | pBac | 200/500 | V229 | (10434) 14325 | 339 | 92 | 27.14 | 26 | 28.26 | 1 | 3.85 |
Ds 16 | pBac | 200/500 | V251 | (10927) 14818 | 413 | 70 | 16.95 | 10 | 14.29 | 0 | 0.00 |
Ds 17 | pBac | 200/500 | V265 | (9952) 13337 | 520 | 124 | 23.85 | 32 | 25.81 | 0 | 0.00 |
avg | 4.65 | ||||||||||
Ds 18 | pBac | 300/700 | V265 | (9952) 13337 | 582 | 238 | 40.89 | 77 | 32.35 | 5 | 6.49 |
Ds 19 | pBac + hyPB | 200+200/500 | V221 | (7867) 11252 | 1457 | 271 | 18.60 | 42 | 15.50 | 1 | 2.38 |
Ds 20 | pBac + hyPB | 200+200/500 | V222 | (7861) 11246 | 1898 | 401 | 21.13 | 34 | 8.48 | 0 | 0.00 |
Ds 21 | pBac + hyPB | 200+200/500 | V223 | (7865) 11249 | 488 | 82 | 16.80 | 30 | 36.59 | 2 | 6.67 |
avg | 3.02 | ||||||||||
Ds 22 | hyPB | 200/500 | V209 | (5538) 8922 | 1081 | 252 | 23.31 | 37 | 14.68 | 2 | 5.41 |
Ds 23 | hyPB | 200/500 | V265 | (9952) 13337 | 98 | 32 | 32.65 | 8 | 25.00 | 0 | 0.00 |
avg | 2.70 |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp) | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total no. G1 screened | no. transg. events * | no. transg. events/ G0 | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | plasmid | 300/150 | AH452 *** | (5267) 8649 | 623 | 29.21 | 78.57 | 8 | 8 | 4 | n.d. | 4 | 1 | 2.80 |
2 | plasmid | 200/500 | V3 | (3545) 6911 | 234 | 31.20 | 86.30 | 25 | 13 | 1 | 8830 | 1 | 1 | 1.59 |
3 | plasmid | 400/600 | V285 | (5180) 8682 | 705 | 25.82 | 64.29 | 18 | 16 | 2 | 14966 | 2 | 1 | 1.71 |
4 | plasmid | 300/500 | V286 | (5600) 9102 | 680 | 13.38 | 41.76 | 15 | 9 | 0 | 8772 | 0 | n.a. | 0.00 |
5 | plasmid | 160/185 | V258 | (6190) 9690 | 922 | 10.41 | 67.71 | 18 | 15 | 0 | 6631 | 0 | n.a. | 0.00 |
6 | plasmid | 400/600; 228/342 | V258 | (6190) 9690 | 599 | 24.21 | 58.62 | 11 | 11 | 0 | 18721 | 0 | n.a. | 0.00 |
7 | plasmid | 300/150 | V257 | (7088) 10587 | 1113 | 42.59 | 76.37 | 26 | 26 | 2 | 93998 | 2 | n.d. | 0.55 |
8 | plasmid | 200/500 | V19 | (3630) 7163 | 257 | 18.29 | 63.83 | 9 | 9 | 2 | 5185 | 2 | 1 | 6.67 |
9 | plasmid | 300/500 | V19 | (3630) 7163 | 192 | 33.85 | 61.54 | 11 | 7 | 0 | 2679 | 0 | n.a. | 0.00 |
10 | plasmid | 200/500 | V19 | (3630) 7163 | 399 | 13.53 | 61.11 | 13 | 6 | 0 | 1313 | 0 | n.a. | 0.00 |
11 | plasmid | 200/500 | V19 | (3630) 7163 | 462 | 14.72 | 48.53 | 7 | 7 | 2 | 1389 | 2 | 1 | 6.06 |
12 | plasmid | 300/300 | V368 | (5835) 11097 | 257 | 5.84 | 60.00 | 7 | 3 | 0 | 502 | 0 | n.a. | 0.00 |
13 | plasmid | 100/300 | V368 | (5835) 11097 | 148 | 5.41 | 62.50 | 4 | 3 | 0 | 382 | 0 | n.a. | 0.00 |
avg | 1.49 | |||||||||||||
14 | mRNA | 182/300 | V96 | (5278) 8813 | 113 | 11.50 | 46.15 | 6 | 3 | 1 | 370 | 4 | 4 | 66.67 |
15 | mRNA | 182/300 | V97 | (4307) 7841 | 298 | 5.03 | 73.33 | 11 | 4 | 2 | 514 | 7 | ≥ 2 - ≥5 | 63.64 |
16 | mRNA | 300/300 | V370 | (5870) 11131 | 576 | 31.25 | 86.67 | 12 | 12 | 11 | 4388 | > 19 | ≥1 | 12.18 |
17 | mRNA | 300/300 | V369 | (6420) 11684 | 520 | 16.35 | 80.00 | 6 | 6 | 5 | 2173 | > 8 | ≥1 | 11.76 |
18 | mRNA | 300/300 | V19 | (3630) 7163 | 527 | 8.92 | 48.94 | 28 | 17 | 10 | 1813 | ≥ 24 | 1 - ≥5 | 104.35 ** |
19 | mRNA | 100/300 | V19 | (3630) 7163 | 310 | 5.48 | 70.59 | 9 | 6 | 2 | 1195 | ≥ 7 | ≥3 - ≥4 | 58.33 |
20 | mRNA | 100/300 | V19 | (3630) 7163 | 646 | 1.61 | 80.00 | 7 | 7 | 3 | 1030 | 4 | 1 - 2 | 50.00 |
21 | mRNA | 300/300 | V368 | (5835) 11097 | 449 | 7.35 | 66.67 | 18 | 10 | 5 | 1014 | ≥ 12 | ≥2 - 4 | 54.55 |
22 | mRNA | 100/300 | V368 | (5835) 11097 | 281 | 4.98 | 85.71 | 7 | 6 | 5 | 870 | ≥ 9 | 1 - ≥3 | 75.00 |
avg | 49.02 |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total G1 screened | no. transg events | no. transg. events/ G0 | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | mRNA | 300/150 | AH452 modified * | (5205) 7845 | 721 | 39.53 | 83.51 | 19 | 19 | 15 | 7049 | ≥ 15 | ≥ 1 | 6.3 % |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total G1 screened | no. transg. events | recomb. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | phsp-phiC31 | 150/300 | V101 | (4404) 8216 | 510 | 3.14 | 75.00 | 9 | 6 | 0 | 1690 | 0 | 0 |
2 | phsp-phiC31 | 150/300 | V101 | (4404) 8216 | 237 | 7.59 | 61.11 | 5 | 5 | 0 | 2851 | 0 | 0 |
3 | mRNA | 150/300 | V101 | (4404) 8216 | 502 | 4.38 | 59.09 | 11 | 4 | 0 | 534 | 0 | 0 |
4 | mRNA | 150/300 | V101 | (4404) 8216 | 335 | 3.28 | 54.55 | 4 | 2 | 0 | 458 | 0 | 0 |
5 | mRNA | 150/300 | V101 | (4404) 8216 | 361 | 3.32 | 83.33 | 7 | 4 | 1 * | 318 | 1 | 10 |
6 | phsp-phiC31 | 300/500 | V101 | (4404) 8216 | 443 | 2.71 | 91.67 | 9 | 4 | 0 | 1059 | 0 | 0 |
7 | phsp-phiC31 | 300/500 | V101 | (4404) 8216 | 302 | 13.25 | 77.50 | 11 | 9 | 2 | 6068 | 2 | 6.45 |
8 | mRNA | 300/500 | V101 | (4404) 8216 | 412 | 2.18 | 100.00 | 9 | 9 | 0 | 6250 | 0 | 0 |
9 | mRNA | 300/500 | V101 | (4404) 8216 | 248 | 13.71 | 73.53 | 10 | 6 | 0 ** | 3650 | 0 | 0 |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total G1 screened | no. transg. events | recomb. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | phsp-Cre | 450/350 | V20 | (1297) 4997 | 765 | 18.69 | 57.34 | 13 | 12 | 2 * | 9283 | 2 | 2.44 |
2 | phsp-Cre | 450/350 | V20 | (1297) 4997 | 165 | 6.67 | 72.73 | 4 | 1 | 0 | 390 | 0 | 0 |
3 | phsp-Cre | 450/350 | V20 | (1297) 4997 | 397 | 1.26 | 80.00 | 3 | 1 | 0 | 137 | 0 | 0 |
4 | mRNA | 450/350 | V20 | (1297) 4997 | 413 | 10.90 | 73.33 | 4 | 1 | 1 ** | 2419 | 1 | 3.03 |
5 | mRNA | 450/350 | V20 | (1297) 4997 | 230 | 5.22 | 58.33 | 3 | 3 | 0 | 996 | 0 | 0 |
6 | mRNA | 450/350 | V20 | (1297) 4997 | 348 | 5.75 | 80.00 | 12 | 9 | 0 | 4926 | 0 | 0 |
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supplementary.zip (201.51KB )
Submitted:
31 August 2023
Posted:
01 September 2023
You are already at the latest version
A peer-reviewed article of this preprint also exists.
supplementary.zip (201.51KB )
Submitted:
31 August 2023
Posted:
01 September 2023
You are already at the latest version
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp) | no. injected embryos | larval hatch rate (%) | adult eclosion rate (%) | total no. G0 families | % fertile G0 families | no. transg. G0 families | total no. G1 screened | no. transg. events | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | pBac | 300/150 | AH452 * | (5267) 8649 | 623 | 29.21 | 78.57 | 8 | 100.00 | 4 | n.d. | 4 | 2.80 |
2 | pBac | 200/500 | V3 | (3545) 6911 | 234 | 31.20 | 86.30 | 25 | 52.00 | 1 | 8830 | 1 | 1.25 |
3 | pBac | 400/600 | V285 | (5180) 8682 | 705 | 25.82 | 64.29 | 18 | 88.89 | 2 | 14966 | 2 | 1.71 |
4 | pBac | 300/500 | V286 | (5600) 9102 | 680 | 13.38 | 41.76 | 15 | 60.00 | 0 | 8772 | 0 | 0.00 |
5 | pBac | 160/185 | V258 | (6190) 9690 | 922 | 10.41 | 37.50 | 18 | 83.33 | 0 | 6631 | 0 | 0.00 |
6 | pBac | 400/600; 228/342 | V258 | (6190) 9690 | 599 | 24.21 | 34.48 | 11 | 100.00 | 0 | 18721 | 0 | 0.00 |
7 | pBac | 300/150 | V257 | (7088) 10587 | 1113 | 42.59 | 76.37 | 26 | 100.00 | 2 | 93998 | 2 | 0.55 |
8 | pBac | 200/500 | V19 | (3630) 7163 | 257 | 18.29 | 63.83 | 9 | 100.00 | 2 | 5185 | 2 | 6.67 |
9 | pBac | 300/500 | V19 | (3630) 7163 | 192 | 33.85 | 61.54 | 11 | 63.64 | 0 | 2679 | 0 | 0.00 |
10 | pBac | 200/500 | V19 | (3630) 7163 | 399 | 13.53 | 61.11 | 13 | 46.15 | 0 | 1313 | 0 | 0.00 |
11 | pBac | 200/500 | V19 | (3630) 7163 | 462 | 14.72 | 48.53 | 7 | 100.00 | 2 | 1389 | 2 | 6.06 |
12 | pBac | 300/300 | V368 | (5835) 11097 | 257 | 5.84 | 60.00 | 7 | 42.86 | 0 | 502 | 0 | 0.00 |
13 | pBac | 100/300 | V368 | (5835) 11097 | 148 | 5.41 | 62.50 | 4 | 75.00 | 0 | 382 | 0 | 0.00 |
avg | 1.46 | ||||||||||||
14 | HypB | 100/200 | V286 | (5600) 9102 | 594 | 8.08 | 75.00 | 12 | 75.00 | 0 | 9382 | 0 | 0.00 |
15 | HypB | 200/200 | V286 | (5600) 9102 | 730 | 8.63 | 79.37 | 17 | 70.59 | 0 | 7604 | 0 | 0.00 |
16 | HypB | 200/200 | V258 | (6190) 9690 | 895 | 6.82 | 65.57 | 13 | 69.23 | 0 | 5199 | 0 | 0.00 |
avg | 0.00 |
Exp. no. | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp) | no. injected eggs | no. hatched larvae | hatch rate (%) | no. fertile adults | fertile eclosion rate (%) | no. transg. lines | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
Ds 1 | pBac | 200/500 | AH443 | (9191) 12576 | 75 | n. d. | n. d. | 25 | n.d. | 4 | 16.00 |
Ds 2 | pBac | 200/500 | V220 | (7865) 11249 | 1601 | 231 | 14.43 | 65 | 28.14 | 1 | 1.54 |
Ds 3 | pBac | 200/500 | V221 | (7867) 11252 | 443 | 102 | 23.02 | 9 | 8.82 | 0 | 0.00 |
Ds 4 | pBac | 200/500 | V146 | (7118) 10503 | 481 | 173 | 35.97 | 20 | 11.56 | 2 | 10.00 |
Ds 5 | pBac | 200/500 | V183 | (7583) 10968 | 753 | 305 | 40.50 | 43 | 14.10 | 5 | 11.63 |
Ds 6 | pBac | 200/500 | V184 | (9438) 12823 | 640 | 167 | 26.09 | 17 | 10.18 | 0 | 0.00 |
Ds 7 | pBac | 200/500 | V185 | (8493) 11878 | 802 | 346 | 43.14 | 53 | 15.32 | 6 | 11.32 |
Ds 8 | pBac | 200/500 | V188 | (10347) 13732 | 631 | 285 | 45.17 | 55 | 19.30 | 1 | 1.82 |
Ds 9 | pBac | 200/500 | V213 | (10059) 13443 | 538 | 107 | 19.89 | 27 | 25.23 | 0 | 0.00 |
Ds 10 | pBac | 200/500 | V215 | (8204) 11589 | 626 | 173 | 27.64 | 29 | 16.76 | 1 | 3.45 |
Ds 11 | pBac | 200/500 | V226 | (8163) 12054 | 410 | 51 | 12.44 | 9 | 17.65 | 1 | 11.11 |
Ds 12 | pBac | 200/500 | V227 | (10018) 13909 | 493 | 122 | 24.75 | 12 | 9.84 | 1 | 8.33 |
Ds 13 | pBac | 200/500 | V228 | (8607) 12498 | 310 | 97 | 31.29 | 13 | 13.40 | 0 | 0.00 |
Ds 14 | pBac | 200/500 | V250 | (9072) 12963 | 378 | 129 | 34.13 | 28 | 21.71 | 0 | 0.00 |
Ds 15 | pBac | 200/500 | V229 | (10434) 14325 | 339 | 92 | 27.14 | 26 | 28.26 | 1 | 3.85 |
Ds 16 | pBac | 200/500 | V251 | (10927) 14818 | 413 | 70 | 16.95 | 10 | 14.29 | 0 | 0.00 |
Ds 17 | pBac | 200/500 | V265 | (9952) 13337 | 520 | 124 | 23.85 | 32 | 25.81 | 0 | 0.00 |
avg | 4.65 | ||||||||||
Ds 18 | pBac | 300/700 | V265 | (9952) 13337 | 582 | 238 | 40.89 | 77 | 32.35 | 5 | 6.49 |
Ds 19 | pBac + hyPB | 200+200/500 | V221 | (7867) 11252 | 1457 | 271 | 18.60 | 42 | 15.50 | 1 | 2.38 |
Ds 20 | pBac + hyPB | 200+200/500 | V222 | (7861) 11246 | 1898 | 401 | 21.13 | 34 | 8.48 | 0 | 0.00 |
Ds 21 | pBac + hyPB | 200+200/500 | V223 | (7865) 11249 | 488 | 82 | 16.80 | 30 | 36.59 | 2 | 6.67 |
avg | 3.02 | ||||||||||
Ds 22 | hyPB | 200/500 | V209 | (5538) 8922 | 1081 | 252 | 23.31 | 37 | 14.68 | 2 | 5.41 |
Ds 23 | hyPB | 200/500 | V265 | (9952) 13337 | 98 | 32 | 32.65 | 8 | 25.00 | 0 | 0.00 |
avg | 2.70 |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp) | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total no. G1 screened | no. transg. events * | no. transg. events/ G0 | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | plasmid | 300/150 | AH452 *** | (5267) 8649 | 623 | 29.21 | 78.57 | 8 | 8 | 4 | n.d. | 4 | 1 | 2.80 |
2 | plasmid | 200/500 | V3 | (3545) 6911 | 234 | 31.20 | 86.30 | 25 | 13 | 1 | 8830 | 1 | 1 | 1.59 |
3 | plasmid | 400/600 | V285 | (5180) 8682 | 705 | 25.82 | 64.29 | 18 | 16 | 2 | 14966 | 2 | 1 | 1.71 |
4 | plasmid | 300/500 | V286 | (5600) 9102 | 680 | 13.38 | 41.76 | 15 | 9 | 0 | 8772 | 0 | n.a. | 0.00 |
5 | plasmid | 160/185 | V258 | (6190) 9690 | 922 | 10.41 | 67.71 | 18 | 15 | 0 | 6631 | 0 | n.a. | 0.00 |
6 | plasmid | 400/600; 228/342 | V258 | (6190) 9690 | 599 | 24.21 | 58.62 | 11 | 11 | 0 | 18721 | 0 | n.a. | 0.00 |
7 | plasmid | 300/150 | V257 | (7088) 10587 | 1113 | 42.59 | 76.37 | 26 | 26 | 2 | 93998 | 2 | n.d. | 0.55 |
8 | plasmid | 200/500 | V19 | (3630) 7163 | 257 | 18.29 | 63.83 | 9 | 9 | 2 | 5185 | 2 | 1 | 6.67 |
9 | plasmid | 300/500 | V19 | (3630) 7163 | 192 | 33.85 | 61.54 | 11 | 7 | 0 | 2679 | 0 | n.a. | 0.00 |
10 | plasmid | 200/500 | V19 | (3630) 7163 | 399 | 13.53 | 61.11 | 13 | 6 | 0 | 1313 | 0 | n.a. | 0.00 |
11 | plasmid | 200/500 | V19 | (3630) 7163 | 462 | 14.72 | 48.53 | 7 | 7 | 2 | 1389 | 2 | 1 | 6.06 |
12 | plasmid | 300/300 | V368 | (5835) 11097 | 257 | 5.84 | 60.00 | 7 | 3 | 0 | 502 | 0 | n.a. | 0.00 |
13 | plasmid | 100/300 | V368 | (5835) 11097 | 148 | 5.41 | 62.50 | 4 | 3 | 0 | 382 | 0 | n.a. | 0.00 |
avg | 1.49 | |||||||||||||
14 | mRNA | 182/300 | V96 | (5278) 8813 | 113 | 11.50 | 46.15 | 6 | 3 | 1 | 370 | 4 | 4 | 66.67 |
15 | mRNA | 182/300 | V97 | (4307) 7841 | 298 | 5.03 | 73.33 | 11 | 4 | 2 | 514 | 7 | ≥ 2 - ≥5 | 63.64 |
16 | mRNA | 300/300 | V370 | (5870) 11131 | 576 | 31.25 | 86.67 | 12 | 12 | 11 | 4388 | > 19 | ≥1 | 12.18 |
17 | mRNA | 300/300 | V369 | (6420) 11684 | 520 | 16.35 | 80.00 | 6 | 6 | 5 | 2173 | > 8 | ≥1 | 11.76 |
18 | mRNA | 300/300 | V19 | (3630) 7163 | 527 | 8.92 | 48.94 | 28 | 17 | 10 | 1813 | ≥ 24 | 1 - ≥5 | 104.35 ** |
19 | mRNA | 100/300 | V19 | (3630) 7163 | 310 | 5.48 | 70.59 | 9 | 6 | 2 | 1195 | ≥ 7 | ≥3 - ≥4 | 58.33 |
20 | mRNA | 100/300 | V19 | (3630) 7163 | 646 | 1.61 | 80.00 | 7 | 7 | 3 | 1030 | 4 | 1 - 2 | 50.00 |
21 | mRNA | 300/300 | V368 | (5835) 11097 | 449 | 7.35 | 66.67 | 18 | 10 | 5 | 1014 | ≥ 12 | ≥2 - 4 | 54.55 |
22 | mRNA | 100/300 | V368 | (5835) 11097 | 281 | 4.98 | 85.71 | 7 | 6 | 5 | 870 | ≥ 9 | 1 - ≥3 | 75.00 |
avg | 49.02 |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total G1 screened | no. transg events | no. transg. events/ G0 | transf. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | mRNA | 300/150 | AH452 modified * | (5205) 7845 | 721 | 39.53 | 83.51 | 19 | 19 | 15 | 7049 | ≥ 15 | ≥ 1 | 6.3 % |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total G1 screened | no. transg. events | recomb. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | phsp-phiC31 | 150/300 | V101 | (4404) 8216 | 510 | 3.14 | 75.00 | 9 | 6 | 0 | 1690 | 0 | 0 |
2 | phsp-phiC31 | 150/300 | V101 | (4404) 8216 | 237 | 7.59 | 61.11 | 5 | 5 | 0 | 2851 | 0 | 0 |
3 | mRNA | 150/300 | V101 | (4404) 8216 | 502 | 4.38 | 59.09 | 11 | 4 | 0 | 534 | 0 | 0 |
4 | mRNA | 150/300 | V101 | (4404) 8216 | 335 | 3.28 | 54.55 | 4 | 2 | 0 | 458 | 0 | 0 |
5 | mRNA | 150/300 | V101 | (4404) 8216 | 361 | 3.32 | 83.33 | 7 | 4 | 1 * | 318 | 1 | 10 |
6 | phsp-phiC31 | 300/500 | V101 | (4404) 8216 | 443 | 2.71 | 91.67 | 9 | 4 | 0 | 1059 | 0 | 0 |
7 | phsp-phiC31 | 300/500 | V101 | (4404) 8216 | 302 | 13.25 | 77.50 | 11 | 9 | 2 | 6068 | 2 | 6.45 |
8 | mRNA | 300/500 | V101 | (4404) 8216 | 412 | 2.18 | 100.00 | 9 | 9 | 0 | 6250 | 0 | 0 |
9 | mRNA | 300/500 | V101 | (4404) 8216 | 248 | 13.71 | 73.53 | 10 | 6 | 0 ** | 3650 | 0 | 0 |
Exp. no | helper template | [helper/ donor] (ng/µl) | donor construct | donor plasmid (insert) size (bp | no. injected embryos | hatch rate (%) | eclosion rate (%) | total no. G0 families | no. fertile G0 families | no. transg. G0 families | total G1 screened | no. transg. events | recomb. eff. (%) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | phsp-Cre | 450/350 | V20 | (1297) 4997 | 765 | 18.69 | 57.34 | 13 | 12 | 2 * | 9283 | 2 | 2.44 |
2 | phsp-Cre | 450/350 | V20 | (1297) 4997 | 165 | 6.67 | 72.73 | 4 | 1 | 0 | 390 | 0 | 0 |
3 | phsp-Cre | 450/350 | V20 | (1297) 4997 | 397 | 1.26 | 80.00 | 3 | 1 | 0 | 137 | 0 | 0 |
4 | mRNA | 450/350 | V20 | (1297) 4997 | 413 | 10.90 | 73.33 | 4 | 1 | 1 ** | 2419 | 1 | 3.03 |
5 | mRNA | 450/350 | V20 | (1297) 4997 | 230 | 5.22 | 58.33 | 3 | 3 | 0 | 996 | 0 | 0 |
6 | mRNA | 450/350 | V20 | (1297) 4997 | 348 | 5.75 | 80.00 | 12 | 9 | 0 | 4926 | 0 | 0 |
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