Röss, H.; Aaldijk, D.; Vladymyrov, M.; Odriozola, A.; Djonov, V. Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus. Int. J. Mol. Sci.2023, 24, 16703.
Röss, H.; Aaldijk, D.; Vladymyrov, M.; Odriozola, A.; Djonov, V. Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus. Int. J. Mol. Sci. 2023, 24, 16703.
Röss, H.; Aaldijk, D.; Vladymyrov, M.; Odriozola, A.; Djonov, V. Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus. Int. J. Mol. Sci.2023, 24, 16703.
Röss, H.; Aaldijk, D.; Vladymyrov, M.; Odriozola, A.; Djonov, V. Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus. Int. J. Mol. Sci. 2023, 24, 16703.
Abstract
Intussusceptive pillars, regarded as a hallmark of intussusceptive angiogenesis, have been described in developing vasculature of many organs and organisms. The aim of this study was to resolve the question about pillar formation and their further maturation employing zebrafish caudal vein plexus (CVP). The CVP development was monitored by in vivo confocal microscopy in high spatio-temporal resolution using the transgenic zebrafish model Fli1a:eGPF//Gata1:dsRed. We tracked back the formation of pillars (diameter ≤4µm) and intercapillary meshes (diameter >4µm) and analysed their morphology and behaviour. Transluminal pillars in the CVP arose by a combination of sprouting, lumen expansion, and/or by creation of intraluminal folds, and those mechanisms were not associated directly with blood flow. The follow-up of pillars indicated that one third of them disappeared between 28-48 hours post fertilisation (hpf) and of the remaining ones only 1/17 changed their cross-section area by >50%. The majority of the bigger meshes (39/62) increased their cross-section area by >50%. Plexus simplification and establishment of hierarchy was dominated by the dynamics of intercapillary meshes, which formed mainly by sprouting angiogenesis. These meshes were observed to grow, reshape, and merge with each other. Our observations suggested an alternative view on intussusceptive angiogenesis in the CVP.
Biology and Life Sciences, Cell and Developmental Biology
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