Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Approach of Supercritical Carbon Dioxide for the Extraction of Kleeb Bua Daeng Formula

Nittaya Ngamkhae
,
Orawan Monthakantirat
,
Yaowared Chulikhit
,
Juthamart Maneenet
ORCID logo ,
Charinya Khamphukdee
ORCID logo ,
Yutthana Chotritthirong
ORCID logo ,
Suphatson Limsakul
,
Chantana Boonyarat
ORCID logo ,
Supaporn Pitiporn
,
Pakakrong Kwankhao
ORCID logo ,
Anake Kijjoa
ORCID logo ,
Supawadee Daodee
* ORCID logo
Version 1 : Received: 24 August 2023 / Approved: 24 August 2023 / Online: 25 August 2023 (11:43:23 CEST)

A peer-reviewed article of this Preprint also exists.

Ngamkhae, N.; Monthakantirat, O.; Chulikhit, Y.; Maneenet, J.; Khamphukdee, C.; Chotritthirong, Y.; Limsakul, S.; Boonyarat, C.; Pitiporn, S.; Kwankhao, P.; Kijjoa, A.; Daodee, S. Approach of Supercritical Carbon Dioxide for the Extraction of Kleeb Bua Daeng Formula. Molecules 2023, 28, 6873. Ngamkhae, N.; Monthakantirat, O.; Chulikhit, Y.; Maneenet, J.; Khamphukdee, C.; Chotritthirong, Y.; Limsakul, S.; Boonyarat, C.; Pitiporn, S.; Kwankhao, P.; Kijjoa, A.; Daodee, S. Approach of Supercritical Carbon Dioxide for the Extraction of Kleeb Bua Daeng Formula. Molecules 2023, 28, 6873.

Abstract

Supercritical fluid extraction (SFE) is an innovative green technology for the extraction of phytochemicals from plants. Therefore, this study aimed to evaluate the application of SFE and to optimize the extraction condition of the Thai herbal formula, Kleeb Bua Daeng (KBD). A Box-Behnken design (BBD) with response surface methodology was used to determine the effect of extraction time, temperature, and pressure on response variables including extraction yield, total phenolic content (TPC), total flavonoid content (TFC), total carotenoid content (TCC) and total anthocyanin content (TAC) of KBD formula. The highest percentage extraction yield, TPC, TFC, and TCC of the extracts were 3.81%, 464.56 mg gallic acid equivalents/g extract, 217.19 mg quercetin equivalents/g extract, and 22.26 mg β-carotene equivalents/g extract, respectively. The results indicated that SFE is a suitable method of extraction for green recovery of some phytochemicals from the KBD formula.

Keywords

Supercritical fluid extraction; response surface methodology; Box-Behnken design; Kleeb Bua Daeng formula

Subject

Chemistry and Materials Science, Applied Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.