Version 1
: Received: 23 August 2023 / Approved: 23 August 2023 / Online: 24 August 2023 (09:51:49 CEST)
How to cite:
Jiang, X.; Yu, S.; Huang, Y.; Huang, J.; Liu, S.; Yang, D.; Fu, J.; He, H.; Fu, H. Identification of Rice RRM1 Gene Family and Its Resistance to Rice Blast. Preprints2023, 2023081684. https://doi.org/10.20944/preprints202308.1684.v1
Jiang, X.; Yu, S.; Huang, Y.; Huang, J.; Liu, S.; Yang, D.; Fu, J.; He, H.; Fu, H. Identification of Rice RRM1 Gene Family and Its Resistance to Rice Blast. Preprints 2023, 2023081684. https://doi.org/10.20944/preprints202308.1684.v1
Jiang, X.; Yu, S.; Huang, Y.; Huang, J.; Liu, S.; Yang, D.; Fu, J.; He, H.; Fu, H. Identification of Rice RRM1 Gene Family and Its Resistance to Rice Blast. Preprints2023, 2023081684. https://doi.org/10.20944/preprints202308.1684.v1
APA Style
Jiang, X., Yu, S., Huang, Y., Huang, J., Liu, S., Yang, D., Fu, J., He, H., & Fu, H. (2023). Identification of Rice RRM1 Gene Family and Its Resistance to Rice Blast. Preprints. https://doi.org/10.20944/preprints202308.1684.v1
Chicago/Turabian Style
Jiang, X., Haohua He and Haihui Fu. 2023 "Identification of Rice RRM1 Gene Family and Its Resistance to Rice Blast" Preprints. https://doi.org/10.20944/preprints202308.1684.v1
Abstract
In order to enhance understanding of RNA-binding proteins in rice, a comprehensive investigation was conducted on the RRM1 gene family of rice, encompassing genome-wide identification and exploration of its role in rice blast resistance. Physical and chemical properties of the OsRRM1 gene family in rice was analyzed, including conserved domain, motif, location information, gene structure, phylogenetic tree, collinearity analysis, cis-acting elements, GO, and KEGG. Furthermore, the expression patterns of the OsRRM1 gene were examined at different time intervals following rice blast treatment. Furthermore, the alterations in expression patterns of selected OsRRM1 genes were assessed using quantitative real-time PCR(qRT-PCR). A total of 212 members of the OsRRM1 gene family were identified, which were dispersed across 12 chromosomes. Many of these genes exhibit multiple exons and introns, all of which encompass the conserved RRM1 domain and share analogous motifs. This observation suggests a high degree of conservation within the encoded sequence domain of these genes. Phylogenetic analysis revealed the existence of five subfamilies within the OsRRM1 gene family. Furthermore, the investigation of the promoter region identified homeopathic elements that are involved in nucleic acid binding and interaction with multiple transcription factors. By employing GO and KEGG analysis, four RRM1 genes were tentatively identified as crucial contributors to plant immunity, while the RRM1 gene family was also found to have a significant involvement in the complex of alternative splicing. Additionally, gene expression analysis indicated that the majority of OsRRM1 genes exhibited constitutive expression. The results of the qRT-PCR analysis revealed distinct temporal changes in the expression pattern of the OsRRM1 gene following rice blast treatment. These findings contribute to the existing knowledge of the OsRRM1 gene family, establish a foundation for further investigation into the role of the OsRRM1 gene in response to rice blast infection, and hold theoretical significance for future studies on the functionality of the OsRRM1 gene.
Keywords
rice; gene family; rice blast; bioinformatics.
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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