Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New-Delhi-Metallo-β-Lactamase Producing Enterobacterales Biofilms

Geiziane Aparecida Gonçalves
,
Victoria Stadler Tasca Ribeiro
ORCID logo ,
Leticia Ramos Dantas
,
Ana Paula De Andrade
,
Paula Hansen Suss
ORCID logo ,
Maria Alice Witt
,
Felipe Francisco Tuon
*
Version 1 : Received: 21 August 2023 / Approved: 22 August 2023 / Online: 23 August 2023 (07:19:07 CEST)

A peer-reviewed article of this Preprint also exists.

Gonçalves, G.A.; Ribeiro, V.S.T.; Dantas, L.R.; de Andrade, A.P.; Suss, P.H.; Witt, M.A.; Tuon, F.F. Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms. Compounds 2023, 3, 584-595. Gonçalves, G.A.; Ribeiro, V.S.T.; Dantas, L.R.; de Andrade, A.P.; Suss, P.H.; Witt, M.A.; Tuon, F.F. Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms. Compounds 2023, 3, 584-595.

Abstract

Background: Antibiofilm activity of silver nanoparticles has been extensively investigated in common bacteria. Metallo-β-lactamase producing Gram-negative bacteria are hard-to-treat microorganisms with few therapeutic options, and silver nanoparticles were not evaluated on the biofilm of these bacteria. Objectives: The aim of this study was to evaluate the antibiofilm activity of a bone scaffold impregnated with silver nanoparticles in NDM-producing Gram-negative bacilli. Methods: Bone scaffolds from bovine femur were used for the tests and impregnated with silver nanoparticles (50 nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentrations (MIC and MBC) were performed on NDM-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Disc diffusion tests for silver nanoparticles susceptibility and quantification of biofilm production on plate and bone with sessile cell count were performed. Results: The MIC results demonstrated that silver nitrate had an antimicrobial effect on all microorganisms, inactivating the growth of isolates from a concentration of 8 µg/mL. MBC results showed that E. coli 16.211 was the only isolate to present MIC different from MBC, with a value of 16 µg/mL. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce biofilm, and it can be a strategic material to be used as an implant for different approaches.

Keywords

Biofilm; silver; bone; nanotechnology; impregnation; bacteria; NDM; scaffold

Subject

Chemistry and Materials Science, Nanotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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